30 results about "Fructokinase" patented technology

The invention relates to the use of isoform-specific fructokinase (ketohexokinase) (KHK) inhibitors alone or in combination with various agents to both prevent and treat a wide variety of diseases including, but not limited to, sugar craving, obesity, features of metabolic syndrome (including insulin resistance, hypertriglyceridemia, hypertension, and fatty liver), polyuria, proximal tubular injury, and diabetic kidney disease.

Belonging to the technical field of bioengineering, the invention relates to a genetic engineering strain producing succinic acid and a method for production of succinic acid by fermenting the strain, particularly a recombinant strain efficiently utilizing cane sugar and molasses to grow and produce succinic acid. The genetic engineering strain producing succinic acid is classified and named as Escherichia coli BA501, and has a preservation registration number of CCTCC NO:M2014014. The construction process of the strain mainly includes: taking Escherichia coli AFP111 that lacks lactate dehydrogenase gene and pyruvate formate lyase activity and has the chromosome ptsG gene undergoing spontaneous mutation as the starting strain, expressing exogenous sucrose permease, sucrose hydrolase and fructokinase genes, and then carrying out continuous domestication cultivation to obtain the strain efficiently utilizing cane sugar and molasses to grow and produce succinic acid. Thus, the synthesis efficiency of succinic acid is greatly improved. The fermentation method adopts a two-stage fermentation way, in the aerobic stage the biomass is improved oxygen, and in the anaerobic stage, fermentation and acid production are achieved.

The invention relates to transgenic plants exhibiting increased cell wall content. In one embodiment, transgenic plants engineered to over-express fructokinase (FRK) or both FRK and sucrose synthase (SuSy) are provided. The FRK+SuSy double-transgenic plants of the invention consistently exhibit enhanced cell wall polymer deposition.

Belonging to the technical field of bioengineering, the invention relates to a genetic engineering strain producing succinic acid and a method for production of succinic acid by fermenting the strain, particularly a recombinant strain efficiently utilizing cane sugar and molasses to grow and produce succinic acid. The genetic engineering strain producing succinic acid is classified and named as Escherichia coli BA501, and has a preservation registration number of CCTCC NO:M2014014. The construction process of the strain mainly includes: taking Escherichia coli AFP111 that lacks lactate dehydrogenase gene and pyruvate formate lyase activity and has the chromosome ptsG gene undergoing spontaneous mutation as the starting strain, expressing exogenous sucrose permease, sucrose hydrolase and fructokinase genes, and then carrying out continuous domestication cultivation to obtain the strain efficiently utilizing cane sugar and molasses to grow and produce succinic acid. Thus, the synthesis efficiency of succinic acid is greatly improved. The fermentation method adopts a two-stage fermentation way, in the aerobic stage the biomass is improved oxygen, and in the anaerobic stage, fermentation and acid production are achieved.

The present invention provides: a lactic acid-producing Escherichia coli comprising at least one gene of a sucrose non-PTS gene group, including at least a sucrose hydrolase gene, provided that a combination of a repressor protein (cscR), a sucrose hydrolase (cscA), a fructokinase (cscK) and a sucrose permease (cscB) and a combination of a sucrose hydrolase (cscA), a fructokinase (cscK) and a sucrose permease (cscB) are excluded, wherein the lactic acid-producing Escherichia coli comprises a lactic acid production enhancing system provided by genetic recombination; and a lactic acid production method including producing lactic acid from a plant-derived sucrose-containing raw material by using the lactic acid-producing Escherichia coli.

High concentration of free cells of heterofermentative lactic acid bacteria (LAB) in a resting or slowly growing state are used to convert fructose into mannitol. Efficient volumetric mannitol productivities and mannitol yields from fructose are achieved in a process applying cell-recycle, continuous stirred tank reactor and / or circulation techniques with native LAB cells or with LAB cells with inactivated fructokinase gene(s). Mannitol is recovered in high yield and purity with the aid of evaporation and cooling crystallization.

The invention discloses a composite native microorganism domestication enzyme for efficiently improving urban black and odorous water bodies and a method for treating urban black and odorous water bodies. The composite native microorganism domestication enzyme includes acetyl CoA acyltransferase, 1-phosphofructokinase and pyruvate phospho-dikinase. , β-hydroxybutyryl-CoA dehydrogenase, 3-phosphoglycerate kinase, NAD-dependent β-hydroxybutyl-CoA dehydrogenase and thiolase. A method for treating urban black and odorous water bodies includes the following steps: preparing and activating compound native microorganism domestication enzymes; putting the activated compound native microorganism domestication enzymes in black and smelly water bodies for a period of time; and then constructing aquatic plants. The composite indigenous microbial domestication enzyme of the present invention has the advantages of efficiently improving the quality of black and odorous sewage; in addition, the treatment method of the present invention has the advantages of efficiently purifying the water body, and sustainably adjusting and optimizing the water body environment.

The invention relates to application of a cyanobacteria fructokinase inactivating agent in preparation of cyanobacteria with fructose secretion capability and high fructose yield, and further relates to a reagent for knocking out a fructokinase gene corresponding to fructokinase in the cyanobacteria or a fructokinase function antagonist. The invention also relates to the fructokinase-disabled cyanobacteria and application of the fructokinase-disabled cyanobacteria in fructose production. According to the invention, fructokinase in a carbon circulation path of cyanobacteria is knocked out, a mutant strain accidentally obtains the capacity of mass production of fructose and secretion of fructose out of cells, the fructose yield is increased by hundreds of times, and the possibility is provided for subsequent industrial production of fructose by using cyanobacteria; and a foundation is provided for further metabolic engineering modification by taking cyanobacteria as a chassis algal strain.

The invention discloses a method for biocatalytically synthesizing D-(+)-glucuronic acid and its application. In the method, sucrose is used as a substrate, and an enzyme is added to carry out a catalytic reaction to obtain D-(+)-glucuronic acid; wherein, the enzymes include sucrose phosphorylase, phosphoglucomutase, inositol-1-phosphate Synthetase, myo-inositol monophosphatase and myo-inositol oxidase, the enzyme catalyzed system can get a high conversion rate, in addition, by adding phosphate, phosphofructokinase and phosphoglucose isomerase to the above reaction system, it can be Further improve the conversion rate of D-(+)-glucuronic acid. The method of the invention has high raw material utilization rate, high conversion rate of D-(+)-glucuronic acid, simple steps, low production cost, little pollution and little impact on the environment, and can realize large-scale D-(+)-glucuronic acid Production.

The invention relates to a method for determining ammonia (ammonia ion) content by utilizing an enzymatic colorimetric method and enzyme-linked immunosorbant assay and composition and components of a reagent for determining ammonia (ammonia ion) content. The technical principle of the method is as follow: determination is completed based on serial catalytic reactions of ammonia kinase, fructokinase and fructose dehydrogenase. The invention also relates to an ammonia (ammonia ion) diagnosis / determination kit. The method has the advantages of high sensitivity and few errors; therefore the method and the kit can be widely applied to clinical medicine / food inspection.

The invention discloses a construction method and application of an engineering bacterial strain for producing tagatose. The construction method reduces the enzymatic activity of fructose 6-phosphate kinase in cells by regulating glucose intracellular metabolism, and enhances the enzymatic activity of glucokinase and glucose 6-phosphate isomerase so as to increase the content of intracellular fructose 6-phosphate. ‑Phosphotagatose 3‑epimerase and 6‑phosphate tagatose phosphorylase for tagatose synthesis pathway, fructose metabolism pathway consisting of fructose permeation enzyme and fructokinase; obtained a glutamine The recombinant strain of Corynebacterium acid can metabolize glucose, fructose or sucrose to synthesize tagatose. Compared with the currently reported method of bioconverting fructose to synthesize tagatose, it has the advantages of simple operation and easy separation, and is suitable for large-scale production of tagatose. sugar.

The invention relates to an ammonia (ammonia ion) content determination method employing an enzymatic colorimetric method and an enzyme-linked technology. The invention also relates to the composition and components of a reagent. The technological principle of the determination is that: the determination is achieved with a series of catalytic reactions of ammonia kinase, ketohexokinase fructokinase and mannitol 2-dehydrogenase. The invention also relates to an ammonia (ammonia ion) diagnosis / determination kit. The determination method provided by the invention is advantaged in high sensitivity and low error. Therefore, the determination method and the kit provided by the invention can be widely applied in clinical medicine / food examination.

The present invention relates to Corynebacterium sp. that is transformed with an Escherichia sp.-derived fructokinase gene to express fructokinase showing a sufficient activity of converting fructose into fructose-6-phosphate, thereby preventing unnecessary energy consumption, and a method for producing L-amino acids using the strain. The transformed Corynebacterium sp. of the present invention is able to express fructokinase from the Escherichia-derived fructokinase gene to prevent unnecessary energy consumption during fructose metabolism, leading to more cost-effective production of L-amino acids. Therefore, it can be widely used for the effective production of L-amino acids.