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A sodium alginate gel microsphere embedded with quantum dots and enzymes, its preparation method and its application in biochemical detection

A technology of sodium alginate and gel microspheres, applied in the field of preparation of biomedical materials, can solve the problem that quantum dots are difficult to use in complex biological samples

Active Publication Date: 2021-09-14
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a sodium alginate gel microsphere embedded with quantum dots and enzymes, its preparation method and its application in biochemical detection, so as to solve the current situation that quantum dots are difficult to be used in the analysis of complex biological samples

Method used

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  • A sodium alginate gel microsphere embedded with quantum dots and enzymes, its preparation method and its application in biochemical detection
  • A sodium alginate gel microsphere embedded with quantum dots and enzymes, its preparation method and its application in biochemical detection
  • A sodium alginate gel microsphere embedded with quantum dots and enzymes, its preparation method and its application in biochemical detection

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Embodiment 1

[0051] A preparation method and condition optimization of sodium alginate gel microspheres embedded with CdTe quantum dots, comprising the steps of:

[0052] 1) Preparation of gel microspheres: put sodium alginate and ultrapure water in a round bottom flask and stir for 10-20 minutes to obtain a uniform viscous solution of 1% sodium alginate by mass; The CdTe quantum dot solution was slowly dropped in at a speed of 3 s / d to make it uniformly dispersed to obtain a viscous solution of sodium alginate with a concentration of 1 μmol / L CdTe quantum dots. Use a pipette gun to drop it into the cross-linking ion solution at a volume of 60 μL per drop, and after standing for a period of cross-linking, sodium alginate gel microspheres embedded with CdTe quantum dots with regular shape and uniform particle size are obtained. After removing the filtrate by filtration, put the gel microspheres in a 4°C refrigerator for sealed storage;

[0053] 2) Selection of microsphere cross-linking ion...

Embodiment 2

[0060] A preparation method of sodium alginate gel microspheres embedded with CdZnTeS quantum dots and its detection of hydrogen peroxide, comprising the following steps:

[0061] 1) Preparation of gel microspheres: put sodium alginate and ultrapure water in a round bottom flask and stir for 10-20 minutes to obtain a uniform viscous solution of 1% sodium alginate by mass; Slowly drop the CdZnTeS quantum dot solution at a speed of 3 s / d to make it uniformly dispersed to obtain a viscous solution of sodium alginate with a concentration of 1 μmol / L CdZnTeS quantum dots. Use a pipette to drop 0.100M Ba at a volume of 60 μL per drop 2+In the solution, after static cross-linking for 30 minutes, sodium alginate gel microspheres with regular shape and uniform particle size embedded with CdZnTeS quantum dots were obtained. After removing the filtrate by filtration, put the gel microspheres in a 4°C refrigerator for sealed storage;

[0062] 2) Add sodium alginate gel microspheres embe...

Embodiment 3

[0067] A preparation method of sodium alginate gel microspheres embedding CdZnTeS quantum dots and its influence on the stability of CdZnTeS quantum dots, comprising the steps of:

[0068] 1) Preparation of gel microspheres: put sodium alginate and ultrapure water in a round bottom flask and stir for 10-20 minutes to obtain a uniform viscous solution of 1% sodium alginate by mass; Slowly drop the CdZnTeS quantum dot solution at a speed of 3 s / d to make it uniformly dispersed to obtain a viscous solution of sodium alginate with a concentration of 1 μmol / L CdZnTeS quantum dots. Use a pipette to drop 0.100M Ba at a volume of 60 μL per drop 2+ In the solution, after static cross-linking for 30 minutes, sodium alginate gel microspheres with regular shape and uniform particle size embedded with CdZnTeS quantum dots were obtained. After removing the filtrate by filtration, put the gel microspheres in a 4°C refrigerator for sealed storage;

[0069] 2) Within 0-60 minutes, 5 μL of Cd...

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Abstract

The invention discloses a sodium alginate gel microsphere embedded with quantum dots and enzymes, a preparation method thereof and an application in biochemical detection. In this method, quantum dots and enzymes are directly added to the viscous solution of sodium alginate, mixed evenly, and then Ba 2+ In the solution, statically cross-link to obtain gel microspheres. Based on the enzymatic reaction in the sodium alginate gel microspheres and the fluorescence quenching characteristics of quantum dots, the naked-eye visual detection of biomolecules in biological samples can be realized under the excitation of ultraviolet light, so as to realize the early screening of corresponding diseases. The obtained gel microspheres have the characteristics of long fluorescence stability time and strong ability to resist protein interference. In addition, the analysis method proposed by the invention is easy to operate, fast and controllable; it shows good application prospects in biochemical detection. The present invention exhibits certain universality in naked-eye visual detection of certain physiological parameters and early screening of corresponding diseases.

Description

technical field [0001] The invention belongs to the technical field of preparation of biomedical materials, and relates to a sodium alginate gel microsphere embedded with quantum dots and enzymes, a preparation method thereof and an application in biochemical detection. Background technique [0002] Sodium alginate (C 6 h 7 o 6 Na)n is mainly a copolymer composed of different numbers of β-D-mannuronic acid (M unit) and α-L-guluronic acid (G unit) connected by β-1,4-glycosidic bonds. When Ca 2+ 、Ba 2+ , Zn 2+ In the presence of such cations, the Na on the G unit + An ion exchange reaction occurs with divalent cations, and the G units stack to form a cross-linked network structure, thereby forming a sodium alginate hydrogel. Due to the mild conditions of the process, enzyme inactivation can be avoided (Gao Chunmei et al., Advances in Chemistry, 2013, 25(06): 1012-1022). Sodium alginate has good modifiability, and after functionalization of biomolecules, it can be used ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/10C12N11/04G01N21/64
CPCC12N9/0006C12N9/0048C12N9/0059C12N9/1096C12N9/6427C12N11/04C12N11/10C12Y101/03004C12Y107/03003C12Y110/03001C12Y206/01002C12Y304/21004G01N21/6428
Inventor 何治柯卢钒吉邢虎
Owner WUHAN UNIV
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