Method for producing ethanol by fermentation of starch raw materials

A technology of starch raw material and ethanol, applied in the field of yeast fermentation, can solve the problems of complex method and high cost, and achieve the effects of increasing the culture temperature, the method is simple, and the cost is low

Inactive Publication Date: 2020-05-05
JILIN COFCO BIOCHEM +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for ethanol fermentation in order to overcome the existing problems of complex and costly methods for improving yeast heat resistance in the prior art, which can improve the heat resistance of yeast so that it can be used at higher temperatures (37-39°C) for growth and metabolism, and the method is simple and low cost

Method used

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  • Method for producing ethanol by fermentation of starch raw materials
  • Method for producing ethanol by fermentation of starch raw materials
  • Method for producing ethanol by fermentation of starch raw materials

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preparation example Construction

[0050] The preparation method of the low-temperature-preserved yeast liquid is as follows: add 1 g of dry yeast to 200 mL of sterile water and shake well, take 2 mL of it and add it to 200 mL of LYPD medium after 10 minutes, cultivate at 28 ° C for 9 hours, and then add 20% glycerol (v / v), stored at -70°C. When in use, melt at room temperature to obtain yeast liquid.

[0051] The liquefying enzyme is a high-temperature resistant α-amylase purchased from Novozymes , the enzyme activity is 12300U / mL. Glucoamylase was purchased from Novozymes , the enzyme activity is 13060U / mL. The protease is the acid protease purchased from Suleibao The enzyme activity is 50000U / g.

[0052] Seed medium (YPD medium) (g / L): Yeast extract 10, peptone 20, glucose 20, glucose solution is sterilized alone at 115°C for 20 minutes, other components of the medium are sterilized at 121°C for 20 minutes, mixed on a sterile operating table before use .

Embodiment 1

[0060] This embodiment is used to illustrate the method for producing ethanol of the present invention

[0061] Prepare 100mL slurry in a 250mL Erlenmeyer flask, and the concentration of rice flour is 94g / L. Add liquefaction enzyme (add-on is 65.4U / g rice flour), glucoamylase (add-on is 69.5U / g rice flour), acid protease (add-on is 228U / g rice flour) and urea (add-on is 1.94 mg / g rice flour), prepared as a liquid medium.

[0062] Use sulfuric acid to adjust the initial pH of the medium to 5, inoculate the yeast liquid stored at low temperature into the above-mentioned Erlenmeyer flask with a 2% (v / v) inoculum, and carry out the initial fermentation with air for 8 hours. Be 170rpm, regularly weigh, record shake flask mass loss (g), its result sees figure 1 .

[0063] Wherein, in embodiment 1-1, rice husk powder is added to the liquid medium, so that compared with rice flour, the content of rice husk powder is 5.6% by weight;

[0064] In Example 1-2, rice husk powder was add...

Embodiment 2

[0070] This embodiment is used to illustrate the method for ethanol production by cultivating yeast fermentation of the present invention (raw meal method)

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Abstract

The present invention relates to the field of yeast fermentation and discloses a method for producing ethanol by fermentation of starch raw materials. The method comprises the following steps: inoculating yeasts into a liquid culture medium for ethanol fermentation; wherein, the liquid culture medium contains starch raw materials and rice husk powder, and is relative to 100 parts by weight of thestarch raw materials; and use amount of the rice husk powder in the liquid culture medium is 3-25 parts by weight. A raw material method is preferably used for fermentation of ethanol. The method is used for cultivation of yeasts, can increase cultivation temperatures of the yeasts, and thus improves heat resistance of the yeasts, so that the yeasts can grow with relatively high efficiency at hightemperatures (37-39 DEG C) to conduct growth and metabolism and cost of ethanol production process can be reduced.

Description

technical field [0001] The invention relates to the field of yeast fermentation, in particular to a method for producing ethanol by fermenting starch raw materials. Background technique [0002] In actual production, ethanol fermentation needs to maintain a low temperature (28-32 ℃), and the yeast growth will release a lot of heat during the fermentation process. If the temperature is not cooled, it will soon exceed the tolerance temperature of the yeast, generally not exceeding 34 ℃, the fermentation tank is cooled by circulating cooling water, and its working principle is that the return water from the fermentation tank enters the top of the cooling tower and sprays down. This process makes the temperature of the cooling water of the fermenter directly related to the ambient temperature, which is generally only 2-3°C lower than the ambient temperature. However, our country is hot in summer, and the outdoor water temperature can even reach 40°C. The cooling water needs to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/06C12P7/10C12R1/865
CPCC12P7/06C12P7/10Y02E50/10
Inventor 佟毅修志龙李义李凡李伟豪孙振江陈博刘胜李永恒牟英刘辉罗虎王小艳
Owner JILIN COFCO BIOCHEM
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