Recombinant G-17 protein, gene for encoding recombinant protein and application of gene for encoding recombinant protein

Abstract

The invention provides recombinant G-17 protein, a gene for encoding the recombinant protein and application of the gene for encoding the recombinant protein. An amino acid sequence of the recombinantG-17 protein is shown in SEQ ID NO: 1 as shown in the description, and a base sequence of the gene for encoding the recombinant G-17 protein is shown in SEQ ID NO: 2 as shown in the description. According to the recombinant G-17 protein, the gene for encoding the recombinant protein and application of the gene for encoding the recombinant protein, after a G-17 antigen epitope sequence is connected with a sequence of a B cell epitope of insulinoma-related protein IA-2 and a Th2 cell epitope of a C terminal of P277 through flexible fragments, the amino acid sequence of the recombinant G-17 protein is formed; and mice are immunized against the recombinant G-17 protein, and an ELISA detection antibody titer value reaches 1:106, so that specific detection and recognition of G-17 are achieved,not only is the detection sensitivity enhanced, but also distortion of a detection result is avoided, the probability of subsequent screening of a high-specificity antibody is improved, and the time and the economic cost are saved.

Description

Technical field [0001] The invention belongs to the technical field of biological products, and particularly relates to a recombinant G-17 protein, a gene encoding the recombinant protein, and applications thereof. Background technique [0002] Gastrin is a gastrointestinal hormone secreted mainly by G cells in the gastric antrum and duodenum. It plays an important role in regulating the function of the digestive tract and maintaining its structural integrity. In the human body, more than 95% of gastrin with biological activity is α-amidated gastrin, which mainly contains two isomers G-17 and G-34, of which 80% to 90% are G-17. G-17 is only secreted by G cells in the gastric antrum. Therefore, G-17 is an important indicator of gastric mucosal damage. It combines with cholecystokinin receptor (CCKR) and exerts its biological function through a series of signal transduction. It is mainly involved in stimulating gastric acid secretion and nourishing the mucosa of the gastrointestin...

AI Technical Summary

Problems solved by technology

[0005] The determination of B cell epitopes or peptide haptens is of great significance to the synthesis of peptide vaccines, the preparation of diagnostic reagents, and the screening of monoclonal antibodies. The premise is to have strong immunogenicity, usually in order to increase the immunogenicity of B cell epitopes or peptide haptens, by coupling B cell epitopes or peptide haptens to carrier proteins to increase Th response, this method has some disadvantages place

Images