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A strain of Enterobacter ludwig pn6 and its application

A technology of Enterobacter ludwigii and Enterobacter ludwigia, applied to Enterobacter ludwig PN6 and its application fields, to achieve the effects of excellent strain resources, growth promotion, and strong phosphorus-solubilizing ability

Active Publication Date: 2021-08-31
JIANGXI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, the research on endophytic bacteria has mainly focused on crops, but there are few studies on endophytic bacteria in woody plants, and there are even fewer reports on endophytic bacteria in moso bamboo

Method used

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  • A strain of Enterobacter ludwig pn6 and its application
  • A strain of Enterobacter ludwig pn6 and its application
  • A strain of Enterobacter ludwig pn6 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Isolation and identification of endophytic bacteria PN6 from Phyllostachys pubescens

[0026] The three- to four-year-old moso bamboos with good growth were selected from the moso bamboo plantations in Dagang Forest Farm, Guanshan Forest Farm and Jinggangshan Dajing Forest Farm in Yichun City, Jiangxi Province, respectively. The topsoil was removed, and the root system of Moso bamboo was dug out with a shovel to collect the fine roots of Moso bamboo. Rinse the root system of moso bamboo with distilled water for 3 times, and carry out surface disinfection according to the following steps: soak in 75% v / v alcohol for 1 min, soak in 3.25% v / v sodium hypochlorite for 3 min, soak in 75% v / v alcohol for 30 seconds, and then rinse with sterile water for 3 times . Grind the treated root tissue and collect the grinding liquid. Coat the NA plate with sterile water after the last rinse (the formula of NA medium: 3g of beef extract, 10g of peptone, 5g of NaCl, 16g of ag...

Embodiment 2

[0033] Embodiment 2: Bacterial strain PN6 is to the endogenous antagonism of Phyllostachys pubescens blight pathogenic bacteria

[0034] Use a hole puncher (diameter=5mm) to get the bacteria cake at the edge of the colony of the pathogenic bacteria C.phyllostachydis (C.phyllostachydis) of Phyllostachys pubescens for 5 days, and connect it to the center of the PDA plate, and mark the bacterial strain to be tested activated with the NA slope The line was inoculated on both sides of the PDA plate, and only the pathogenic bacteria were inoculated as the control (CK), and each treatment was replicated three times. Incubate at 28°C for 4 days to observe the presence or absence of the bacteriostatic zone, and measure the width of the bacteriostatic zone.

[0035] Inoculate the Enterobacter ludwigia PN6 with better bacteriostasis in the plate confrontation test on NB medium (the formula of NB medium: beef extract 3g, peptone 10g, NaCl 5g, distilled water 1000mL, pH 7.2, mix well and the...

Embodiment 3

[0039] Embodiment 3: Determination test of bacterial strain PN6 phosphorus-dissolving ability

[0040] Enterobacter PN6 was inoculated on NBRIP solid medium (glucose 10g, Ca 3 (PO 4 ) 2 5g, MgCl 2 5g, KCl 0.2g, MgSO 4 .7H 2 O 0.25g, (NH 4 ) 2 SO 4 0.1g, distilled water 1000mL, agar 15g, mix uniformly and sterilize to obtain), cultivate for 4 days and observe whether phosphorus-dissolving circles are produced. The phosphate-solubilizing ability of Enterobacter ludwigs PN6 was quantified using the method of Pikovskaya et al. Enterobacter PN6 was shaken and cultivated in NB medium for 24 hours, inoculated into a 100mL Erlenmeyer flask containing 50mL of NBRIP culture solution at an inoculum size of 1% v / v, and connected to the NBRIP culture solution of the same volume of blank seed solution (no access to strains) as a control. Each inoculation treatment was repeated five times, 30°C, 180r / min shaking culture for 6 days, the fermentation broth was centrifuged for 10min...

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Abstract

The invention discloses a strain of Enterobacter ludwig PN6 and its application. The taxonomic name of the strain is Enterobacter ludwigii, the strain number is PN6, and it is preserved in the China Center for Type Culture Collection with the preservation number: CCTCC NO: M 2019881. The bacterial strain PN6 screened from the root system of moso bamboo in the present invention has a significant antibacterial effect on the pathogenic fungus of Phyllostachys phalaensis, and the antibacterial rate of its fermentation broth can reach 49.28%, and has high-efficiency phosphorus-solubilizing and IAA-secreting abilities; The bacterial strain PN6 of the present invention has promoting action to the growth of Phyllostachys pubescens, and the test data shows that the liquid inoculant made of PN6 has significant growth-promoting action to growth indexes such as ground diameter, seedling height and chlorophyll of Phyllostachys pubescens, and can increase the stress resistance of Phyllostachys pubescens, Reduce the probability of occurrence of moso bamboo shoot dieback. Therefore, the present invention provides excellent bacterial strain resources for developing environment-friendly moso bamboo biological special fertilizers.

Description

technical field [0001] The invention relates to the technical field of microorganisms and biological fertilizers, in particular to a strain of Enterobacter ludwig PN6 and its application. Background technique [0002] Moso bamboo (Phyllostachys edulis) is the most widely distributed bamboo plant in my country, with the largest stock volume and the most widely used. It is a bamboo species with excellent economic and ecological benefits. In recent years, with the increase in the demand for moso bamboo, the phenomenon of nutrient depletion in moso bamboo plantations has also increased day by day. In addition, moso bamboo will be attacked by various diseases during the growth process, such as moso bamboo sooty disease, moso bamboo rust and moso bamboo shoot dieback disease, among which moso bamboo shoot dieback disease is more harmful. The disease mainly harms the side branches and young shoots of Hsinchu in the same year. Pathogens can invade through natural orifices or wounds...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/20A01P1/00A01P21/00C05G3/00C05G3/60C12P17/10C12R1/01
CPCC05G3/00C12P17/10C12N1/205C12R2001/01
Inventor 陈伏生张扬毛瑢方向民卜文圣
Owner JIANGXI AGRICULTURAL UNIVERSITY
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