Process method for extracting three enzymes from animal pancreas

A process method, pancreas technology, applied in the field of bioengineering, can solve the problems of high price, low product purity, and other enzyme structure damage, and achieve the effects of reducing resource waste, high product purity, and easy operation
CN111117989AActive Publication Date: 2020-05-08江西浩然生物制药有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
江西浩然生物制药有限公司
Publication Date
2020-05-08

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Abstract

The invention discloses a process method for extracting three enzymes from animal pancreas. The process method comprises the following steps: by taking animal pancreas as a raw material, performing tissue crushing, activation, soaking, filtration, ultrafiltration, anion exchange chromatography and hydrophobic chromatography so as to obtain high-purity pancreatic kallidinogenase; performing cation-exchange chromatography on an anion exchange chromatography column-through fluid so as to obtain elastase; activating Sepharose, arming aminocaproic acid, coupling o-aminoanisole so as to obtain an O-Anisidin-ACA-Sepharose affinity adsorbent, and adsorbing a cation-exchange chromatography column-through fluid, so as to obtain high-purity chymotrypsin. By adopting the method, co-production of threeenzymes, namely pancreatic kallidinogenase, chymotrypsin and elastase, can be achieved, the operation is simple and convenient, the product purity is high, and resource waste can be reduced. Due to synthesis and use of an O-Anisidin-ACA-Sepharose medium, the production cost is reduced, and the product quality is improved.
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Description

technical field

[0001] The invention belongs to the technical field of bioengineering, in particular to a process method for extracting three kinds of enzymes from animal pancreas. Background technique

[0002] Pancreatic Kallidinogenase (Pancreatic Kallidinogenase), also known as pancreatic kallikrein and kallikrein, is a kind of endo-type proteolytic enzyme, which exists in various tissues in mammals, especially in the pancreas. Rich. Pancreatic kininogenase is a glycoprotein composed of 18 amino acids and 4 sugars, with a molecular weight of about 26800 and an isoelectric point of 4.05.

[0003] Chymotrypsin, also known as chymotrypsin, is a kind of protease extracted from animal pancreas, which belongs to serine peptide chain endonuclease, and its isoelectric point is 8.1.

[0004] Elastase is a broad-spectrum proteolytic enzyme characterized by decomposing insoluble elastin, with an isoelectric point of 9.5, mainly found in animal pancreas, skin, aorta, platelets and ...

Claims

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