A method and device for detecting the infection degree of Botrytis cinerea in fruit

A detection device and technology for Botrytis cinerea, which are applied in measurement devices, biochemical equipment and methods, and microbial determination/inspection, etc., can solve the problems of long running time, inability to be widely used in fruit and food, and expensive, and achieve intuitive results. , Control the effect of further spread, rapid detection

Active Publication Date: 2021-06-29
ZHEJIANG UNIV
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  • Application Information

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Problems solved by technology

[0007] At present, the existing methods for analyzing volatile substances mainly include the combination of gas chromatography and flame ionization detector (GC-FID), or the combination of mass spectrometry (GC-MS) when the type of volatile substances needs to be identified. Combined with headspace solid-phase microextraction (HS-SPME); this type of analytical technique has a long overall run time, is expensive and cannot be widely used in fruit and food testing

Method used

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  • A method and device for detecting the infection degree of Botrytis cinerea in fruit
  • A method and device for detecting the infection degree of Botrytis cinerea in fruit
  • A method and device for detecting the infection degree of Botrytis cinerea in fruit

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Embodiment 1

[0052] Detection of Volatile Substances in Strawberry Infected with Botrytis cinerea

[0053] 1.1 Experimental materials

[0054] Strawberries (Fragaria×ananassa): purchased from the local market, transported to the laboratory within 3 hours for follow-up operations: immerse and wash with 0.5% sodium hypochlorite for 4 minutes, then soak and rinse twice with water, 2 minutes each time, under natural ventilation conditions Leave to air dry.

[0055] Botrytis cinerea (Bc05.10) spore suspension: suspend and dilute with 0.1% Tween 80 to obtain 5×105 cfu / mL gray mold spore suspension.

[0056] 1.2 Strawberry infection

[0057] Experimental group: Soak the washed strawberries in Botrytis cinerea spore suspension for 10 seconds, take them out, place them under natural ventilation for 2 hours, cultivate them in an incubator at 25°C and 90% humidity for 2 days, take pictures every day and freeze them save.

[0058] Control group: Soak the washed strawberries in 0.1% Tween 80 for 10...

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Abstract

The invention discloses a method and a device for detecting the degree of gray mold infection of fruit, belonging to the technical field of fruit preservation. In the method, the volatile substances after the fruit is infected with gray mold are detected, and the degree of fruit infection with gray mold is divided ; Take the content of the detected volatile substance as the independent variable x, and take the degree of fruit infection Botrytis cinerea as the dependent variable y to draw a graph to obtain the functional relationship between x and y; use the detected volatile substance as the detection index to design an electronic The nasal detection device detects the content of volatile substances after fruit is infected with Botrytis cinerea; the detected content of volatile substances is substituted into a function relationship to obtain the degree of fruit infection with Botrytis cinerea. In the method for detecting the infection degree of Botrytis cinerea in fruit provided by the present invention, by measuring the types and contents of volatile substances in fruits, it is checked whether the fruit is infected with Botrytis cinerea, which is beneficial to control the further spread of Botrytis cinerea, and can better protect the quality of fruits.

Description

technical field [0001] The invention relates to the technical field of fruit preservation, in particular to a method and a device for detecting the infection degree of Botrytis cinerea in fruit by measuring components of volatile substances. Background technique [0002] Botrytis cinerea, also known as Botrytis cinerea, is a wide-hosted fungus that can cause damping, leaf fall, flower rot, fruit rot, and rotten cellars in a variety of plant seedlings, fruits, and storage organs. Botrytis cinerea produces a large number of gray mold layers (conidiophores and conidia) on the surface of the gray mold, called gray mold, which is widely distributed in the air and can not only infect field crops, but also affect the postharvest stage of plants. cause huge losses. [0003] According to statistics, up to 25% of fruit spoilage is caused by Botrytis cinerea, which damages mature or aging tissues in fruits the most; during fruit planting, storage and transportation, if some fruits are...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/00G01N30/02G01N30/86C12Q1/6851
CPCC12Q1/6851G01N27/00G01N30/02G01N30/86G01N30/8679C12Q2531/113C12Q2521/107C12Q2561/113
Inventor 罗自生徐艳群李莉章晓晨童志超
Owner ZHEJIANG UNIV
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