Method and device for detecting infection degree of fruit botrytis cinerea
A detection device and technology for Botrytis cinerea, which are applied in measurement devices, biochemical equipment and methods, and determination/inspection of microorganisms, etc., can solve problems such as inability to widely use fruits and foods, high price, and long running time, and achieve intuitive results. , the effect of protecting quality and reducing loss
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[0052] Detection of Volatile Substances in Strawberry Infected with Botrytis cinerea
[0053] 1.1 Experimental materials
[0054] Strawberries (Fragaria×ananassa): purchased from the local market, transported to the laboratory within 3 hours for follow-up operations: immerse and wash with 0.5% sodium hypochlorite for 4 minutes, then soak and rinse twice with water, 2 minutes each time, under natural ventilation conditions Leave to air dry.
[0055] Botrytis cinerea (Bc05.10) spore suspension: suspend and dilute with 0.1% Tween 80 to obtain 5×105 cfu / mL gray mold spore suspension.
[0056] 1.2 Strawberry infection
[0057] Experimental group: Soak the washed strawberries in Botrytis cinerea spore suspension for 10 seconds, take them out, place them under natural ventilation for 2 hours, cultivate them in an incubator at 25°C and 90% humidity for 2 days, take pictures every day and freeze them save.
[0058] Control group: Soak the washed strawberries in 0.1% Tween 80 for 10...
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