Method and device for detecting infection degree of fruit botrytis cinerea

A detection device and technology for Botrytis cinerea, which are applied in measurement devices, biochemical equipment and methods, and determination/inspection of microorganisms, etc., can solve problems such as inability to widely use fruits and foods, high price, and long running time, and achieve intuitive results. , the effect of protecting quality and reducing loss

Active Publication Date: 2020-05-08
ZHEJIANG UNIV
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Problems solved by technology

[0007] At present, the existing methods for analyzing volatile substances mainly include the combination of gas chromatography and flame ionization detector (GC-FID), or the combination of mass spectrometry (GC-MS) when the type of volatile substances needs to be identified. Combined with headspace solid-phase microextraction (HS-SPME); this type of analytical technique has a long overall run time, is expensive and cannot be widely used in fruit and food testing

Method used

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  • Method and device for detecting infection degree of fruit botrytis cinerea
  • Method and device for detecting infection degree of fruit botrytis cinerea
  • Method and device for detecting infection degree of fruit botrytis cinerea

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Embodiment 1

[0052] Detection of Volatile Substances in Strawberry Infected with Botrytis cinerea

[0053] 1.1 Experimental materials

[0054] Strawberries (Fragaria×ananassa): purchased from the local market, transported to the laboratory within 3 hours for follow-up operations: immerse and wash with 0.5% sodium hypochlorite for 4 minutes, then soak and rinse twice with water, 2 minutes each time, under natural ventilation conditions Leave to air dry.

[0055] Botrytis cinerea (Bc05.10) spore suspension: suspend and dilute with 0.1% Tween 80 to obtain 5×105 cfu / mL gray mold spore suspension.

[0056] 1.2 Strawberry infection

[0057] Experimental group: Soak the washed strawberries in Botrytis cinerea spore suspension for 10 seconds, take them out, place them under natural ventilation for 2 hours, cultivate them in an incubator at 25°C and 90% humidity for 2 days, take pictures every day and freeze them save.

[0058] Control group: Soak the washed strawberries in 0.1% Tween 80 for 10...

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Abstract

The invention, which belongs to the technical field of fruit fresh keeping, discloses a method and device for detecting the infection degree of fruit botrytis cinerea. According to the method, volatile substances obtained after fruits are infected with botrytis cinerea are detected, and the infection degrees of the fruits with botrytis cinerea are classified; a graph is drawn by taking the contentof the detected volatile substances as an independent variable x and taking the degree of the fruit infected with the botrytis cinerea as a dependent variable y and a function relationship between the x and the y is calculated; an electronic nose detection device is designed by taking the detected volatile substances as detection indexes, and the content of the volatile substances after the fruits are infected with botrytis cinerea is detected; and the detected content of the volatile substances is substituted into the function relationship to obtain the degree of infection of the fruit withthe botrytis cinerea. According to the method for detecting the infection degree of the fruit botrytis cinerea, whether the fruit is infected with the botrytis cinerea or not is checked by measuring the variety and content of volatile substances of the fruit, so that further diffusion of the botrytis cinerea is controlled, and the quality of the fruit is better protected.

Description

technical field [0001] The invention relates to the technical field of fruit preservation, in particular to a method and a device for detecting the infection degree of Botrytis cinerea in fruit by measuring components of volatile substances. Background technique [0002] Botrytis cinerea, also known as Botrytis cinerea, is a wide-hosted fungus that can cause damping, leaf fall, flower rot, fruit rot, and rotten cellars in a variety of plant seedlings, fruits, and storage organs. Botrytis cinerea produces a large number of gray mold layers (conidiophores and conidia) on the surface of the gray mold, called gray mold, which is widely distributed in the air and can not only infect field crops, but also affect the postharvest stage of plants. cause huge losses. [0003] According to statistics, up to 25% of fruit spoilage is caused by Botrytis cinerea, which damages mature or aging tissues in fruits the most; during fruit planting, storage and transportation, if some fruits are...

Claims

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Application Information

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IPC IPC(8): G01N27/00G01N30/02G01N30/86C12Q1/6851
CPCC12Q1/6851G01N27/00G01N30/02G01N30/86G01N30/8679C12Q2531/113C12Q2521/107C12Q2561/113
Inventor 罗自生徐艳群李莉章晓晨童志超
Owner ZHEJIANG UNIV
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