IV-type collagen reagent detection kit and preparation method thereof

A technology of collagen and detection box, which is applied in the direction of measuring devices, biological testing, material inspection products, etc., and can solve the problems of cumbersome detection methods, human injury, and high detection costs

Pending Publication Date: 2020-05-08
四川纳海川生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Therefore, in the prior art, radioimmunotherapy is mostly used in the methods for detecting type IV collagen, but the defects of this method are that the detection cost is high, the detection method is cumbersome, and the radioactive detection has certain damage to the human body.

Method used

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  • IV-type collagen reagent detection kit and preparation method thereof
  • IV-type collagen reagent detection kit and preparation method thereof
  • IV-type collagen reagent detection kit and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] A type IV collagen reagent test kit, including reagent R1 and reagent R2;

[0043] The components and concentrations in the reagent R1 are as follows:

[0044]

[0045] The solvent is water;

[0046] The components and concentrations in the reagent R2 are as follows:

[0047]

[0048]

[0049] The solvent is water.

[0050] The preparation method of the above-mentioned type IV collagen test kit includes the following steps:

[0051] Step A). ​​Preparation of reagent R1:

[0052] According to the components of reagent R1, add sodium chloride, PEG-2000, gum arabic, trehalose, Tween-80, sodium azide, sucrose, and magnesium chloride to the Tris buffer solution and mix thoroughly, then add the solvent water After adding, continue to stir the mixture, adjust the pH of the solution to 7.8, and filter the mixture solution with a microporous membrane to obtain the filtrate as reagent R1;

[0053] Step B). Preparation of reagent R2:

[0054] Add BSA, glycerol, and Tween-80 to the Tris buffer ...

Embodiment 2

[0056] A type IV collagen reagent test kit, including reagent R1 and reagent R2;

[0057] The components and concentrations in the reagent R1 are as follows:

[0058]

[0059]

[0060] The solvent is water;

[0061] The components and concentrations in the reagent R2 are as follows:

[0062]

[0063] The solvent is water.

[0064] The preparation method of the above-mentioned type IV collagen test kit includes the following steps:

[0065] Step A). ​​Preparation of reagent R1:

[0066] According to the components of reagent R1, add sodium chloride, PEG-2000, gum arabic, trehalose, Tween-80, sodium azide, sucrose, and magnesium chloride to the Tris buffer solution and mix thoroughly, then add the solvent water After adding, continue to stir the mixture, adjust the pH of the solution to 7.8, and filter the mixture solution with a microporous membrane to obtain the filtrate as reagent R1;

[0067] Step B). Preparation of reagent R2:

[0068] Add BSA, glycerol, and Tween-80 to the Tris buffer ...

Embodiment 3

[0070] A type IV collagen reagent test kit, including reagent R1 and reagent R2;

[0071] The components and concentrations in the reagent R1 are as follows:

[0072]

[0073] The solvent is water.

[0074] The components and concentrations in reagent R2 are as follows:

[0075]

[0076] The solvent is water.

[0077] The preparation method of the above-mentioned type IV collagen test kit includes the following steps:

[0078] Step A). ​​Preparation of reagent R1:

[0079] Add sodium chloride, PEG-2000, gum arabic, trehalose, Tween-80, sodium azide, sucrose, and magnesium chloride to the Tris buffer solution according to the components of reagent R1. After adding, continue to stir the mixture, adjust the pH of the solution to 7.8, and filter the mixture solution with a microporous membrane to obtain the filtrate as reagent R1;

[0080] Step B). Preparation of reagent R2:

[0081] Add BSA, glycerin, and Tween-80 to the Tris buffer solution in turn, mix the materials evenly, add type IV coll...

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Abstract

The invention discloses an IV-type collagen reagent detection kit. The IV-type collagen reagent detection kit comprises a reagent R1 and a reagent R2. The reagent R1 is prepared from the following components: a Tris buffer solution, sodium chloride, PEG-2000, Arabic gum, trehalose, tween-80, sodium azide, cane sugar, magnesium chloride and water serving as a solvent. The components and concentration of the reagent R2 are as follows: a Tris buffer solution, BSA, glycerinum, an IV type collagen polyclonal antibody, tween-80 and water serving as a solvent. Meanwhile, the invention discloses a preparation method of the reagent detection kit, including preparation of the reagent R1 and the reagent R2. According to the technical scheme disclosed by the invention, the technical defects of high detection cost, complicated detection mode and certain harm to a human body of a radioimmunotherapy adopted in the prior art are successfully overcome. The reagent detection kit disclosed by the invention can be used for rapidly, efficiently and accurately detecting the content of IV-type collagen, so that the rapidness and accuracy of clinical diagnosis are effectively improved.

Description

Technical field [0001] The invention relates to the field of preparation of detection kits, in particular to a type IV collagen reagent detection kit and a preparation method thereof. Background technique [0002] Collagen such as type IV collagen belongs to fibrin, and its biological spatial structure is a peptide chain network structure. Type IV collagen is an important biological component of basement membrane. Under normal circumstances, collagen will not be deposited in large amounts in tissue cells, and the production and metabolism of fibrous tissue will maintain the normal metabolic process. [0003] However, when human tissues are affected, especially liver tissues, the production and metabolism of fibrous tissues in the liver fibrous tissues are blocked, and a large amount of deposited collagen such as type IV collagen will be produced. [0004] Therefore, the content of type IV collagen is one of the detection indicators for detecting liver tissue diseases such as liver ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6893G01N33/6887G01N2333/78G01N2800/085
Inventor 廖爱平刘猛胡玉立
Owner 四川纳海川生物科技有限公司
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