Unlock instant, AI-driven research and patent intelligence for your innovation.

Anti-inflammatory pentapeptide, its extraction and separation method and its application in improving memory

A technology for improving memory and neuroinflammation, applied in the field of active peptides, to achieve the effect of relieving brain neuroinflammation and inhibiting activity

Active Publication Date: 2021-08-10
SOUTH CHINA UNIV OF TECH +1
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Conversely, the process will generate more ROS

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Anti-inflammatory pentapeptide, its extraction and separation method and its application in improving memory
  • Anti-inflammatory pentapeptide, its extraction and separation method and its application in improving memory
  • Anti-inflammatory pentapeptide, its extraction and separation method and its application in improving memory

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] BV-2 cell culture

[0032] Fetal bovine serum (FBS) was added to the DMEM medium in an amount of 10% (v / v), and then a mixed double antibody composed of 100 U / mL penicillin and 100 μg / mL streptomycin was added. The cell culture incubator provides the required environment for the growth of BV-2 cells, and the conditions in the incubator are set at 37°C and contain 5% CO 2 . Change the medium and subculture in time according to the growth of the cells.

Embodiment 2

[0034] Synthesis of APTLW Polypeptide by Solid Phase Synthesis

[0035] Swell and wash the dichloro resin, remove the Fmoc protecting group, add amino acid for condensation reaction, repeat the process of removal-protection-condensation until all amino acids are connected. Cut the resin to obtain the crude polypeptide Ala-Pro-Thr-Leu-Trp, and purify it by reverse-phase high-performance liquid chromatography to obtain the pure polypeptide (>95%).

Embodiment 3

[0037] Method for Separating and Purifying APTLW Polypeptide

[0038] (1) Preparation of walnut protein powder: extract walnut protein from walnut dregs by alkali-dissolving and acid-precipitating methods. The specific process is as follows, after walnut meal is powdered, add water to dissolve (material ratio is 1:12) → adjust the pH value of the solution to 8.5 → stir for 2 hours → centrifuge (8000rpm, 15min, 4°C) → take the supernatant and adjust the pH value to 4.5 → stir 2h→centrifugation (8000rpm, 15min, 4°C)→precipitate dissolution→dialysis desalination for 2d (slag and water 1:5)→drying→walnut protein powder.

[0039] (2) Preparation of enzymatic hydrolyzate of walnut protein: Take 1 part of walnut protein powder and 8 parts of deionized water by weight and mix evenly, use two kinds of proteases (composite plant hydrolase and trypsin) to enzymolyze at 55 °C for 12 hours, compound plant The amounts of hydrolase and trypsin added were 1.0% and 1.0% (w / w), respectively. ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an anti-inflammatory pentapeptide, its extraction and separation method and its application in improving memory. The amino acid sequence of the anti-inflammatory pentapeptide is Ala-Pro-Thr-Leu-Trp, and its extraction and separation method comprises the following steps: Alkali-soluble acid precipitation extracts walnut protein from walnut dregs, takes walnut protein, mixes it with deionized water, enzymatically hydrolyzes it, and takes the supernatant to obtain a walnut protein hydrolyzate; uses a 3kDa molecular weight ultrafiltration membrane to collect the permeate; walnut protein enzymatically hydrolyzes The low molecular weight components of the solution were loaded on a gel filtration chromatographic column, eluted with deionized water, and the detection wavelength was 220nm, collected and combined into multiple elution peak components, and the components with strong anti-inflammatory activity were selected for mass spectrometry detection. Confirmed to contain the anti-inflammatory pentapeptide of the present invention. The anti-inflammatory pentapeptide of the present invention has good inhibitory activity on LPS-stimulated BV-2 cell inflammatory response, and can be used to prepare memory-improving drugs or memory-improving health care products.

Description

technical field [0001] The invention belongs to the field of active peptides, and in particular relates to an anti-inflammatory pentapeptide, its extraction and separation method and its application in improving memory. Background technique [0002] Microglia are the resident macrophages of the central nervous system (CNS) and play key roles in innate immune regulation and neuronal homeostasis. However, under conditions of brain neuroinflammation, activated microglia induce the release of pro-inflammatory mediators, including nitric oxide (NO), prostaglandin E 2 (PGE 2 ) and proinflammatory cytokines, such as interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α), etc. They play a key role in neurodegenerative diseases. As a major cytokine, IL-1β has an important role in the control of innate and adaptive immune responses. It can enhance the expression of other pro-inflammatory cytokines such as TNF-a and IL-6. Overproduction of IL-1β also stim...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06C07K1/36C07K1/34C07K1/16C12P21/06A61K38/08A61P25/28A61P25/00A23L33/18
CPCA23V2002/00A61K38/00A23L33/18A61P25/00A61P25/28C07K7/06C12P21/06A23V2200/322A23V2200/30A23V2250/55
Inventor 赵谋明王曙光苏国万郑淋
Owner SOUTH CHINA UNIV OF TECH