Electrochemical sensor for simultaneously detecting PAT and OTA, preparation method and detection method

A sensor and electrochemical technology, applied in the field of rapid detection of food safety, to achieve the effect of low detection concentration, low cost and simple operation

Inactive Publication Date: 2020-05-15
NORTHWEST UNIV(CN)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Aiming at the defects and deficiencies of the prior art, the present invention provides an electrochemical sensor and a preparation method for simultaneous detection of PAT and OTA, which solves the problem that simultaneous detection of PAT and OTA cannot be realized in existing detection methods

Method used

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  • Electrochemical sensor for simultaneously detecting PAT and OTA, preparation method and detection method
  • Electrochemical sensor for simultaneously detecting PAT and OTA, preparation method and detection method
  • Electrochemical sensor for simultaneously detecting PAT and OTA, preparation method and detection method

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preparation example Construction

[0044] The preparation method of the working electrode of the electrochemical sensor that simultaneously detects PAT and ochratoxin A of the present invention comprises the following steps:

[0045] Step 1, use alumina slurry to polish the working electrode, then ultrasonically clean it several times with a mixture of ethanol and ultrapure water, and finally ultrasonically clean it several times with ultrapure water;

[0046] Step 2, drop-coating the AuNPs-BP nanocomposite prepared by the present invention on the treated working electrode, and drying at room temperature to obtain the AuNPs-BP / GCE electrode;

[0047] Step 3, apply the first mixed solution onto the surface of the AuNPs-BP / GCE electrode, incubate at room temperature for 2-14 hours, then rinse with Tris-HCl, and then coat the surface with 6-mercapto-1-hexanol at room temperature The reaction time is 0.2~2h, which is used to seal the uncovered active sites on the surface of AuNPs-BP / GCE and prevent other electroact...

Embodiment 1

[0056] Preparation of working electrodes for electrochemical sensors that simultaneously detect PAT and OTA:

[0057] Step 1, use alumina slurry to polish the glassy carbon electrode, then use the mixture of ethanol and ultrapure water to ultrasonically clean it several times, and finally use ultrapure water to ultrasonically clean it several times;

[0058] Step 2, centrifugally concentrate the commercially available BP dispersion (0.2 mg / mL) to 2.0 mg / mL, and put it in the refrigerator (temperature 4°C) for later use;

[0059] 350 μL of 10 mM chloroauric acid (HAuCl 4 ) was added to 24.65mL ultrapure water to obtain a uniform mixed solution; the mixed solution was heated on a heating plate to nearly 150°C; then 2.5mL of sodium citrate with a concentration of 10mM was added and reacted for about 25 minutes to obtain a nano-gold solution;

[0060] 2.0 mg / mL BP dispersion and 0.13 mM nano-gold solution were mixed at a volume ratio of 3:1, and reacted for 1 h to obtain AuNPs-BP...

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Abstract

The invention discloses an electrochemical sensor for simultaneously detecting PAT and OTA, a preparation method and a detection method. The electrochemical sensor comprises a working electrode, a reference electrode and a counter electrode, and the surface of the working electrode is sequentially coated with an AuNPs-BP nano composite material layer and an identification sensitive layer for identifying an object to be detected; the AuNPs-BP nano composite material layer is coated on the surface of the working electrode, and the identification sensitive layer is coated on the surface of the AuNPs-BP nano composite material layer; the identification sensitive layer comprises a PAT aptamer chain, a PAT complementary chain, an OTA aptamer chain and an OTA complementary chain. The electrochemical sensor can detect PAT and OTA at the same time, detection of two target objects is achieved through the same electrochemical sensing platform, and the detection concentration is low.

Description

technical field [0001] The invention belongs to the technical field of rapid detection of food safety, and in particular relates to an electrochemical sensor for simultaneous detection of PAT and OTA, a preparation method and a detection method. Background technique [0002] Foodborne illnesses caused by microbial pathogens and biotoxins in food have posed a serious threat to the health of millions of people in recent years, with foodborne illnesses reaching record levels in many countries. Contaminated food can cause food poisoning and, in severe cases, death in humans and animals. Therefore, food safety issues caused by foodborne pathogenic bacteria and the biotoxins they produce have become the focus of attention at home and abroad, and are extremely challenging. [0003] Patulin (PAT) is a toxic secondary metabolite produced by at least 60 species of fungi of the genera Penicillium, Aspergillus, and Phytophthora. It mainly exists in the fruits, vegetables and nuts that...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/327G01N27/48
CPCG01N27/308G01N27/3278G01N27/48
Inventor 盛庆林赵海艳张雪莲岳田利
Owner NORTHWEST UNIV(CN)
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