Fermentation method for improving spore number of bacillus coagulans

A technology of Bacillus coagulans and a fermentation method, which is applied in the field of fermentation to increase the number of Bacillus coagulans spores, can solve the problems of low spore rate and low product quality, and achieves the effects of increasing the production volume and increasing the total number of bacterial cells

Inactive Publication Date: 2020-05-19
GUANGZHOU AONONG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are a few domestic manufacturers trying to produce Bacillus coagulans products, but the spore rate is generally low, resulting in generally low quality of related products. Therefore, it is particularly important to find a process that can increase the spore rate and number of spores

Method used

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  • Fermentation method for improving spore number of bacillus coagulans
  • Fermentation method for improving spore number of bacillus coagulans
  • Fermentation method for improving spore number of bacillus coagulans

Examples

Experimental program
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Effect test

Embodiment 1-5

[0066] Embodiment 1-5 provides a kind of fermentation method that promotes bacillus coagulans spore quantity, it comprises the following steps:

[0067] S1. Preparation of Bacillus coagulans seed solution.

[0068] The Bacillus coagulans was rejuvenated and activated according to the conventional method, then inoculated into the shake flask culture medium and cultured at 40°C for 48 hours and used as the seed liquid, and the seed liquid was expanded.

[0069] The components of the shake flask medium include: peptone 10g / L, yeast powder 5g / L, glucose 20g / L, sodium acetate 5g / L, diammonium citrate 2g / L, dipotassium hydrogen phosphate 2g / L and the rest water.

[0070] S2. Inoculating Bacillus coagulans into the fermentation medium.

[0071] According to Table 1, the culture media of Examples 1-5 were respectively prepared, and the pH of the culture medium in the fermenter was adjusted to be 7.0-7.2, and the sterilization operation was carried out. Inoculate the expanded seed l...

Embodiment 6

[0080] This embodiment is basically the same as Embodiment 3, the difference is:

[0081] In this example, the components of the shake flask medium include: peptone 8g / L, yeast powder 6g / L, glucose 24g / L, sodium acetate 4g / L, diammonium citrate 1.6g / L, dipotassium hydrogen phosphate 2.4 g / L and the balance of water.

Embodiment 7

[0083] This embodiment is basically the same as Embodiment 3, the difference is:

[0084] In this example, the components of the shake flask medium include: peptone 12g / L, yeast powder 4g / L, glucose 16g / L, sodium acetate 6g / L, diammonium citrate 2.4g / L, dipotassium hydrogen phosphate 1.6 g / L and the balance of water.

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Abstract

The invention discloses a fermentation method for improving the spore number of bacillus coagulans and relates to the technical field of bacterial culture. The method comprises the following steps: inoculating bacillus coagulans into a fermentation culture medium; and performing first feeding and second feeding on the fermentation culture medium after fermentation, wherein the culture medium of afermentation tank comprises a carbon source, a nitrogen source, magnesium salt, manganese salt, sodium salt, potassium salt, lycopene, calcium carbonate and bran. During first feeding, the feeding carbon source and the feeding nitrogen source are added, and during the second feeding, calcium carbonate, bran, corn cobs and amino acids are added. According to the invention, lycopene, calcium carbonate and bran are added as components of the culture medium, so that strain degradation can be inhibited, and bacillus coagulans can be promoted to generate spores. Then feeding is performed twice, andcomponents required by the culture medium in a fermentation process can be supplemented, so that the generation amount of spores is increased, and the total thallus number, the spore rate, the spore number and preservation capacity of the bacillus coagulans in subsequent products of fermentation are improved.

Description

technical field [0001] The invention relates to the technical field of bacterial culture, in particular to a fermentation method for increasing the number of bacillus coagulans spores. Background technique [0002] Bacillus coagulans is a gram-positive bacteria that can form spores and produce lactic acid. The bacterium is rod-shaped, with blunt round ends, single or in short chains, can produce spores, and the spores are terminal. In addition to the ability of general lactic acid bacteria to maintain the balance of intestinal microecology and improve the digestive function and health level of the body, it also has the anti-stress ability of spores such as gastric acid resistance, anti-choline resistance, heat resistance and anti-drying resistance. [0003] In recent years, the use of microecological preparations in breeding has gradually increased, and Bacillus coagulans has gradually attracted people's attention in recent years due to its characteristics of both lactic ac...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/38C12N3/00C12R1/07
CPCC12N1/20C12N1/38C12N3/00
Inventor张志榕兰玉华张诗杨伟春周通吴有林
OwnerGUANGZHOU AONONG BIOTECH