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Synthesis method and application of co-based mof material with tunable pore size with nucleic acid screening function

A nucleic acid molecule and nucleic acid technology, applied in the field of synthesis of Co-based MOF materials, can solve the problems that the interaction of biological macromolecules is in its infancy, and achieve the effects of easy adjustment, simple preparation process, and low cost

Active Publication Date: 2021-08-03
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

MOFs have been used in the fields of small molecule separation, gas storage, catalysis, imaging, drug delivery, etc., but the research on their interaction with biomacromolecules is still in its infancy

Method used

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  • Synthesis method and application of co-based mof material with tunable pore size with nucleic acid screening function
  • Synthesis method and application of co-based mof material with tunable pore size with nucleic acid screening function
  • Synthesis method and application of co-based mof material with tunable pore size with nucleic acid screening function

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Synthesis of MOF ligands

[0047] (1): Add 4-bromo-2-methoxybenzoic acid methyl ester and pinacol diboronic acid ester to the N,N-dimethylformamide solution according to the equivalent ratio of 1:1.5. Heated to 85°C under the same conditions and reacted for 5 hours, the product was separated by column chromatography, and intermediate 1 was obtained after rotary evaporation and drying.

[0048] (2): Add 4-bromo-2-methoxybenzoic acid methyl ester and intermediate 1 to 1,4-dioxane and aqueous solution in an equivalent ratio of 1:1, wherein 1,4-dioxane The volume ratio of water and water is 4:1, heated to 90 degrees under anhydrous and anaerobic conditions for 24 hours, and the product is separated by column chromatography. After rotary evaporation and drying, it is added to 50 equivalents of dichloromethane solution, and the - Add 8 equivalents of boron tribromide solution at 78 degrees, react for 12 hours, then quench with excess water, then add 5 equivalents of 1M sodiu...

Embodiment 2

[0059] Particle size distribution test:

[0060] The dried sample is prepared with a single crystal silicon zero background sample stage, and then the structure is tested on a powder X-ray diffraction instrument.

[0061] Analysis of results:

[0062] figure 1 It is the powder crystal diffraction pattern of Co-based IRMOF-74-II and simulated Co-based IRMOF-74-II in Example 1. It can be seen from the figure that the synthesized Co-based IRMOF-74-II structure conforms to the structure of the matching simulation;

[0063] figure 2 It is the powder crystal diffraction pattern of Co-based IRMOF-74-III and simulated Co-based IRMOF-74-II in Example 1. It can be seen from the figure that the synthesized Co-based IRMOF-74-III structure conforms to the structure of the matching simulation;

[0064] image 3 It is the powder crystal diffraction pattern of Co-based IRMOF-74-IV and simulated Co-based IRMOF-74-IV in Example 1. It can be seen from the figure that the synthesized Co-base...

Embodiment 3

[0071] Selective Adsorption of Nucleic Acids with Different Spatial Size Structures by MOF Materials

[0072] (Step 1) Design nucleic acids with different structures: ssDNA, dsDNA, G4-DNA, ssRNA, G4-RNA, hairpin-RNA. And label these nucleic acid sequences with terminal fluorescent molecules.

[0073] (Step 2) Test the adsorption efficiency of MOF materials to these nucleic acids with different structures

[0074] (Step 2a) Mix 4 μL of annealed DNA / RNA of different structures (10 μM) with 4 μL of Co-IRMOF-74-II, -III, IV (2 mg / mL) in 50 μL of aqueous solution (containing 100 mM KCl and 20 mM KAc, pH 6 .8), react in a mixer at a constant temperature of 37°C for 2 hours.

[0075] (Step 2b) Measure the fluorescence intensity of the supernatant after centrifugation. The absorption efficiency was calculated using the following formula.

[0076]

[0077]

[0078] (FIoriginal is the fluorescence intensity of pure fluorescently labeled DNA / RNA in a buffer solution without MOF ...

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Abstract

The invention relates to a synthesis method and application of a Co-based MOF material with an adjustable pore size having a nucleic acid screening function. The synthesis method comprises the following steps: (1) respectively synthesizing organic ligands II, III and IV with increasing chain lengths shown in the following formula: (2) selecting metal Co to prepare the same topology with the organic ligands II, III and IV respectively, Obtain MOFs materials with increasing pore size; (3) activate the prepared MOFs materials to obtain Co-based MOF materials: Co‑IRMOF‑74‑II, Co‑IRMOF‑74‑III and Co‑IRMOF‑74‑IV. The MOF material synthesized by the present invention is applicable to the rapid separation of nucleic acid molecules of various types of secondary structures, and has universal applicability, and MOF materials with different pore sizes are selected according to different types of isolated nucleic acid structures.

Description

technical field [0001] The present invention relates to the technical field of synthesizing metal-organic framework materials and the technical field of selective separation of nucleic acids with different secondary structures, in particular to a synthesis method and application of a Co-based MOF material with adjustable pore size and nucleic acid screening function. Background technique [0002] Most nucleic acids, including deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), exist in linear and flexible structures, but some of them can also form various rigid secondary structures, such as double helix, G quadruple strand Body (G4)1-4, hairpin structure, triple helix and i-motif structure. The secondary structure of RNA molecules is critical to their biological functions and cellular regulation. The conformational dynamics of RNAs are the basis of RNA regulatory mechanisms and the origin of their complex functions. RNA molecules are able to switch between different se...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08G83/00C12Q1/6806
CPCC08G83/008C12Q1/6806C12Q2523/308
Inventor 周翔邓鹤翔彭双别秉霖
Owner WUHAN UNIV
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