Anti-depression total flavonoid extract as well as preparation method and application thereof

A technology of total flavonoids and extracts, applied in the field of medicine, can solve problems such as side effects, achieve strong antidepressant effects, shorten immobility time, and ensure drug safety

Inactive Publication Date: 2020-05-29
NINGXIA MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these drugs can play a certain antidepressant effect in practice, their use will be accompanied by some side effects.

Method used

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  • Anti-depression total flavonoid extract as well as preparation method and application thereof
  • Anti-depression total flavonoid extract as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0037] The embodiment of the present invention discloses a preparation method of an antidepressant total flavonoid extract, comprising the following steps:

[0038] (1) 20 mesh sieves are crossed after the root and stem of Sophora fragrans are sun-dried to obtain Sophora fragrans powder;

[0039] (2) according to the volume ratio of 1: (5~8) add volume concentration of 70%-80% ethanol to Sophora fragrans powder, heat under reflux, filter, take the filter residue and repeat 1 to 3 times to add extractant, heat under reflux and filter Operation, combining the filtrates, and concentrating to obtain the extract; heating and refluxing at a temperature of 80°C for 2 hours; concentration is at 65°C under reduced pressure until there is no alcohol smell;

[0040] (3) The extract was added to a 60-mesh polyamide column for chromatography, eluted with 30% and 70% ethanol solutions by volume respectively, and the obtained eluent was diluted with water, stirred and allowed to stand for 6-...

Embodiment 1

[0043] Embodiment 1 of the present invention discloses a preparation method of Sophora Sophorae total flavonoids extract, specifically comprising the following steps:

[0044] (1) The roots and rhizomes of Sophora sophora were dried and crushed through a 20-mesh sieve, and the resulting sophora sophora powder was extracted by heating and refluxing with 70% ethanol at a solid-to-liquid ratio of 1:8 for 3 times, each time for 2 hours, The extracts were filtered and combined, and concentrated under reduced pressure at 65°C until there was no alcohol smell;

[0045] (2) Put the concentrate in step (1) on a polyamide chromatographic column for separation and purification, elute with ethanol solutions with a volume concentration of 30% and 70% respectively, and concentrate to obtain an eluent.

[0046] (3) Stir and dilute the concentrated solution obtained in step (2) with water, then let it stand for 6-8h, and centrifuge (3000-5000rpm, 30min) to get the precipitate.

[0047] (4) P...

Embodiment 2

[0048] Embodiment 2 test verification

[0049] One, get the Sophora Sophorae total flavonoids extract that above-mentioned embodiment 1 prepares and carry out the research of mouse SPT experiment

[0050] 1. Experimental method

[0051] After continuous administration for 14 days, behavioral tests were carried out after intragastric administration once a day. The animals were randomly divided into a control group (con), a chronic stress model group (mod), a Sophora flavonoids extract administration group (TF, 100 mg / kg) and an escitalopram group (positive control group, ES , 30mg / kg, intraperitoneal injection), 10 mice in each group. Before the sugar water test, food and water were fasted for 24 hours in advance. All mice were housed in a single cage, and each mouse was given a bottle of 2% sugar water and a bottle of pure water. The sugar water bottle and the pure water bottle exchange positions after one hour. And after two hours, weigh and record the consumption of suga...

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Abstract

The invention discloses a preparation method of an anti-depression total flavonoid extract. The preparation method comprises steps as follows: roots and rhizomes of sophora alopecuroide are dried andcrushed to obtain sophora alopecuroide powder; an extracting agent is added to the sophora alopecuroide powder, heating reflux and filtration are performed, filtration residues are taken, addition ofan extracting agent, heating reflux and filtration operation are repeated, and filtrate is mixed and concentrated to obtain an extract; the extract is sequentially added to a polyamide column and a macroporous resin column for chromatography, an obtained eluant is concentrated, and the total flavonoid extract of the sophora alopecuroide is obtained. According to the total flavonoid extract as wellas a preparation method and an application thereof, the prepared total flavonoid extract is extracted from a natural drug, namely, sophora alopecuroide, has a safe and non-toxic source, has an anti-depression function, can be used for preparing an anti-depression drug, produces no side effects and can overcome defects in the prior art.

Description

technical field [0001] The invention relates to the technical field of medicine, and more particularly to an antidepressant total flavonoid extract and a preparation method and application thereof. Background technique [0002] Depression is one of the most common mental illnesses, characterized by significant and persistent depression, often accompanied by severe psychological and physical disorders, and is extremely harmful to human health. A serious burden on society and families. In addition, patients with depression are often accompanied by insomnia, lethargy, overeating, weight loss, etc., and more severe ones often have some extreme symptoms such as circadian rhythm and endocrine disorders. At present, there are at least 90 million depression patients in my country, and only 5% of them have received treatment; at the same time, among the suicide and suicide attempts in my country, depression patients are as high as 50%-70%, second only to ischemic heart disease. The ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/489A61P25/24A23L33/00A61K125/00A61K135/00
CPCA23V2002/00A61K36/489A61K2236/333A61K2236/53A61K2236/55A23L33/00A61P25/24A23V2200/322
Inventor 王汉卿陶伟伟唐蓉蓉肖东刘颖赵建军雍婧姣王庆贺凯张霞冯军王建寰高晓娟付慧玲柳全霞曾亮杨彩艳
Owner NINGXIA MEDICAL UNIV
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