Method for screening and acclimating high-temperature resistant enterococcus faecalis
A high-temperature-resistant technology of Enterococcus faecalis, which is applied in the direction of using microorganisms, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problems of low drying survival rate of Enterococcus faecalis and ignore the heat-resistant characteristics of strains, etc., to achieve Improve the survival rate of drying, save time and labor costs, and the effect of simple operation procedures
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Embodiment 1
[0034] like figure 1 As shown, the screening and domestication methods of thermostable Enterococcus faecalis are as follows:
[0035] 1) Pick 2-3 rings of bacterial lawn from the strain slope and put them in 100mL liquid medium, and culture them statically at 50°C for 48h.
[0036] 2) Streak-inoculate the culture solution in 1) on the MRS solid medium, and incubate anaerobically at 37° C. for 48 hours.
[0037] 3) Select 2-3 dominant colonies from the streaked plate in 2) for the first time and inoculate them in 100 mL of fresh MRS liquid medium, and culture them statically at 50° C. for 48 hours.
[0038] 4) Streak-inoculate the culture solution in 3) on the MRS solid medium, and incubate anaerobically at 37° C. for 48 hours.
[0039] 5) Select 2-3 dominant colonies from the streaked plate in 4) for the second time and inoculate them in 100 mL of fresh MRS liquid medium, and culture them statically at 50° C. for 48 hours.
[0040] 6) Streak-inoculate the culture solution i...
Embodiment 2
[0064] A method for screening and domesticating high-temperature-resistant Enterococcus faecalis, the method comprising the following steps:
[0065] 1) Pretreatment of Enterococcus faecalis lawn:
[0066] 1-1) Select the bacterial lawn from the slant of the strain and inoculate it in the liquid medium, and then leave it for anaerobic culture at 51°C for 45 hours;
[0067] 1-2) Streak inoculation of the liquid medium on the solid medium, followed by anaerobic culture at 40° C. for 45 hours.
[0068] 2) Select dominant colonies from step 1) and perform 4 times of extreme temperature liquid culture and plate colony screening to obtain thermostable Enterococcus faecalis.
[0069] In step 2), the extreme temperature cultivation process is: inoculate the dominant colonies in the liquid medium, and then statically cultivate anaerobic culture at 51 ° C for 45 hours; the plate colony screening process is: streak the liquid medium on the solid medium , and then cultured anaerobically...
Embodiment 3
[0072] A method for screening and domesticating high-temperature-resistant Enterococcus faecalis, the method comprising the following steps:
[0073] 1) Pretreatment of Enterococcus faecalis lawn:
[0074] 1-1) Select the bacterial lawn from the slant of the strain and inoculate it in a liquid medium, and then place it at 49°C for 50 hours of static anaerobic culture;
[0075] 1-2) Streak inoculation of the liquid medium on the solid medium, followed by anaerobic culture at 35° C. for 50 h.
[0076] 2) Select dominant colonies from step 1) and perform 5 times of extreme temperature liquid culture and plate colony screening to obtain thermostable Enterococcus faecalis.
[0077] In step 2), the extreme temperature cultivation process is: inoculate the dominant colonies in the liquid medium, and then statically cultivate anaerobic culture at 49 ° C for 50 h; the plate colony screening process is: streak the liquid medium on the solid medium , and then cultured anaerobically at ...
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