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Composition for treating esophageal cancer and application thereof

A composition and technology for esophageal cancer, applied in the field of traditional Chinese medicine, can solve problems such as unsystematic research, unclear treatment mechanism, and no active monomer composition ratio relationship, etc., and achieve large market potential and broad market application prospects Effect

Active Publication Date: 2021-08-24
HENAN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the many types of existing traditional Chinese medicine prescriptions, complex components, and unclear therapeutic mechanism, it has caused difficulties for the popularization and application of traditional Chinese medicine.
At present, there are no reports on the most active monomers of pangolin, pig tooth soap, soil Fritillaria, and Chonglou against esophageal cancer, and there is no proportional relationship between the active monomer formulas, and there is no research on the mechanism of action of drugs on esophageal cancer cells. system

Method used

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  • Composition for treating esophageal cancer and application thereof
  • Composition for treating esophageal cancer and application thereof
  • Composition for treating esophageal cancer and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Embodiment 1: the preparation of pangolin active monomer

[0071] 1.1 Extraction method

[0072] (1) Alcohol extraction: Weigh the pangolin, soak it in 95% (v / v) ethanol solution (1g:7ml) at room temperature for 6 days, filter it with suction, discard the filter residue, concentrate, freeze-dry in vacuum at -80°C to obtain a brown yellow solid;

[0073] (2) Extraction: dissolve the brown-yellow solid in ultrapure water (mass volume ratio: 1g: 50ml), and use different polar solvents such as petroleum ether, chloroform, ethyl acetate, and n-butanol to extract in a separating funnel. , the five-part extract (petroleum ether, chloroform, ethyl acetate, n-butanol, water) was rotary evaporated, and vacuum-80°C was freeze-dried to obtain a solid. The activity was tested by MTT method, and the results showed that the n-butanol part of pangolinus was active the strongest;

[0074] (3) macroporous resin chromatographic separation: the solid of n-butanol part is dissolved in ul...

Embodiment 2

[0084] Embodiment 2: the preparation of pig tooth soap active monomer

[0085] 2.1 Extraction method

[0086] (1) Alcohol extraction: Weigh pig tooth soap, soak it with 95% (v / v) ethanol solution (mass volume ratio: 1g: 7ml) at room temperature for 6 days, then filter with suction, discard the filter residue, concentrate, and freeze-dry at -80°C in vacuum A brownish-yellow solid was obtained;

[0087] (2) Extraction: dissolve the brownish yellow solid in ultrapure water (mass volume ratio is 1g: 50ml), use chloroform, ethyl acetate, n-butanol and other different polar solvents to extract successively in a separating funnel, and four Parts of the extract (chloroform, ethyl acetate, n-butanol, water) were rotary evaporated, and vacuum-80°C freeze-dried to obtain a solid. The activity was tested by MTT method, and the results showed that the n-butanol part of the pig tooth soap had the strongest activity;

[0088] (3) macroporous resin chromatographic separation: the solid of n...

Embodiment 3

[0097] Embodiment 3: the preparation of soil Fritillaria active monomer

[0098] 3.1 Extraction method

[0099] (1) Alcohol extraction: Weigh the soil Fritillaria, soak it with 95% (v / v) ethanol solution (mass volume ratio: 1g: 7ml) at room temperature for 6 days, filter with suction, discard the filter residue, concentrate, and freeze in vacuum at -80°C Dry to a brown-yellow solid;

[0100](2) Extraction: dissolve the brownish yellow solid in ultrapure water (mass volume ratio is 1g: 50ml), use chloroform, ethyl acetate, n-butanol and other different polar solvents to extract successively in a separating funnel, and four Parts of the extract (chloroform, ethyl acetate, n-butanol, water) were rotary evaporated, vacuum-80°C freeze-dried to obtain a solid, and the activity was tested by the MTT method, and the results showed that the n-butanol part of the fritillary fritillaria had the strongest activity;

[0101] (3) Chromatographic separation of macroporous resin: the solid ...

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Abstract

The invention discloses a composition for treating esophageal cancer and its application. The technical scheme is that the invention uses a cell model, is guided by activity tracking, and adopts modern extraction and separation technologies such as alcohol extraction, chromatography, and high performance liquid chromatography. Active monomers were extracted from four traditional Chinese medicines with anti-cancer activity: Pangolinus, Zhuyazao, Tubeimu and Chonglou, and the chemical spectrogram method was used to speculate on the molecular composition and determine the molecular structure of each monomer, which was determined by the baseline equal ratio increase and decrease method The dosage ratio of the monomers is combined into a composition for treating esophageal cancer, and then the active monomers and the composition are used to act on various esophageal cancer cells to study their mechanism of action on esophageal cancer. The four active monomers and their compositions provided by the invention are all derived from the extraction of natural medicines, are pure traditional Chinese medicine preparations, have the functions of preventing and treating esophageal cancer, and have great market potential and broad market application prospects.

Description

technical field [0001] The present invention relates to four kinds of active monomers for treating esophageal cancer and their compositions and applications, in particular to active monomers respectively extracted from four kinds of traditional Chinese medicines, namely Chuanshanlong, Zhuyazao, Tu Fritillaria and Chonglou, and the composition for treating esophageal cancer The composition belongs to the technical field of traditional Chinese medicine. Background technique [0002] The medical term for cancer is called malignant tumor, which is intractable, metastatic, harmful, and has high mortality. It is the main fatal non-communicable disease in the 21st century and brings a heavy economic burden to society and families. According to the 2018 Global Cancer Statistical Report, esophageal cancer accounts for 2% of all malignant tumors, ranks seventh in the incidence of malignant tumors, and sixth in overall death, of which male patients account for 75%. The occurrence of e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J63/00C07J71/00A61K36/896A61P35/00
CPCA61K36/42A61K36/483A61K36/8945A61K36/896A61K2236/333A61K2236/39A61K2236/51A61K2236/53A61K2236/55A61P35/00A61K2300/00
Inventor 司富春王文彬司高
Owner HENAN UNIV OF CHINESE MEDICINE
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