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Method for determining content of penicillic acid in fruits

A technology of penicillic acid and fruit, applied in the biological field, can solve the problems of long detection time, cumbersome operation and low sensitivity, and achieve the effect of overcoming cumbersome pretreatment and simple operation.

Active Publication Date: 2020-06-12
ZHAOQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Thin-layer chromatography is difficult to apply to the detection of trace penicillic acid in food
Penicillic acid has a high polarity and a high boiling point, so it cannot be directly analyzed by gas chromatography. It needs to be derivatized by silanization, and the operation is very cumbersome.
The ultraviolet absorption of penicillic acid is weak, and

Method used

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  • Method for determining content of penicillic acid in fruits
  • Method for determining content of penicillic acid in fruits
  • Method for determining content of penicillic acid in fruits

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Accurately weigh 10.0 g of the homogenized pulp of the pitted peaches into a 50 mL plastic centrifuge tube, add 10 mL of ethyl acetate and 3 g of sodium chloride, place on a shaker, and extract by shaking at 200 rpm for 30 min at 25 °C, 3800 rpm Centrifuge for 5min, take 7.5mL supernatant in a clean round-bottomed flask, place it in a rotary evaporator, and keep the temperature of the water bath at room temperature. Acetonitrile to volume.

[0037] Take 1mL constant volume solution in a 2mL centrifuge tube filled with purifying agent (30mg multi-walled carbon nanotubes + 50mg C18), vortex on a vortex instrument for 30s to absorb impurities such as pigments, centrifuge at 10000rpm for 1min, and take the supernatant After passing through a 0.22 μm organic filter membrane, it is to be detected and analyzed by high-performance liquid chromatography triple quadrupole tandem mass spectrometry.

Embodiment 2

[0039] Accurately weigh 10.0 g of the homogenized pulp of the cored apples into a 50 mL plastic centrifuge tube, add 10 mL of ethyl acetate and 3 g of sodium chloride, place on a shaker, and extract by shaking at 200 rpm for 30 min at 25 °C, 3800 rpm Centrifuge for 5min, take 7.5mL supernatant in a clean round-bottomed flask, place it in a rotary evaporator, and keep the temperature of the water bath at room temperature. Acetonitrile to volume.

[0040] Take 1mL constant volume solution in a 2mL centrifuge tube filled with purifying agent (30mg multi-walled carbon nanotubes + 50mg C18), vortex on a vortex instrument for 30s to absorb impurities such as pigments, centrifuge at 10000rpm for 1min, and take the supernatant After passing through a 0.22 μm organic filter membrane, it is to be detected and analyzed by high-performance liquid chromatography triple quadrupole tandem mass spectrometry.

Embodiment 3

[0042] Accurately weigh 10.0 g of the homogenized citrus pulp into a 50 mL plastic centrifuge tube, add 10 mL of ethyl acetate and 3 g of sodium chloride, place on a shaker, extract by shaking at 200 rpm at 25 °C for 30 min, and centrifuge at 3800 rpm for 5 min , take 7.5mL of supernatant in a clean round bottom flask, place it in a rotary evaporator, under the condition of water bath temperature at room temperature, rotary evaporate to near dryness, blow dry with ear wash ball, and fix with 1.5mL chromatographically pure acetonitrile Allow.

[0043] Take 1mL constant volume solution in a 2mL centrifuge tube filled with purifying agent (30mg multi-walled carbon nanotubes + 50mg C18), vortex on a vortex instrument for 30s to absorb impurities such as pigments, centrifuge at 10000rpm for 1min, and take the supernatant After passing through a 0.22 μm organic filter membrane, it is to be detected and analyzed by high-performance liquid chromatography triple quadrupole tandem mass ...

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Abstract

The invention relates to the technical field of biology, in particular to a method for measuring the content of penicillic acid in fruits. The invention discloses a method for determining the contentof penicillic acid in fruits, which comprises the following steps: (1) adding ethyl acetate and sodium chloride into fruit pulp, carrying out oscillating, carrying out centrifuging, carrying out rotary evaporation until the fruit pulp is nearly dry, performing blow-drying with an ear washing bulb, and fixing the volume to obtain a constant volume solution; (2) putting the constant volume liquid into a centrifuge tube filled with a purifying agent, and carrying out vortex, centrifugation and filtration to obtain a sample solution; and (3) detecting the sample solution by using an ultra-high performance liquid chromatography-mass spectrometry method to finally obtain the content of penicillanic acid in fruits. The method provided by the invention overcomes the defects of tedious pretreatmentand long detection time of high performance liquid chromatography, can efficiently, accurately and sensitively obtain the detection result of penicillic acid in fruits by using an ultra-high performance liquid chromatography-mass spectrometry method, and is simple and safe to operate.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for measuring the content of penicillic acid in fruits. Background technique [0002] The molecular formula of penicillic acid is C 8 h 10 o 4 , with a relative molecular weight of 170.16, is a colorless needle-like crystalline compound with a melting point of 83°C, easily soluble in hot water, ethanol, ether and chloroform, insoluble in pentane and hexane. Penicillic acid is mainly a polyacetyl mycotoxin produced by Penicillium arcuatus. It is one of the common mycotoxins, which can inhibit DNA synthesis in animals and can produce joint toxicity with other mycotoxins. During transportation and storage, fruits are easily polluted by Penicillium and become rotten. Therefore, a new method for trace analysis of penicillic acid is established, which can quickly and accurately determine the content of penicillic acid in fruits. Contamination levels of mycolic acid and the set...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/06G01N30/12G01N30/14G01N30/72
CPCG01N30/88G01N30/06G01N30/12G01N30/14G01N30/72G01N2030/884G01N2030/126G01N2030/146Y02A50/30
Inventor 关文碧游颖欣
Owner ZHAOQING UNIV
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