Complex microbial inoculant for regulating gynecological micro-ecological balance

A compound bacterial agent, ecological balance technology, applied in the field of microorganisms, can solve problems such as disease recurrence, and achieve the effect of convenient use

Active Publication Date: 2020-06-16
KUIMING JIAJIANING BIOLOGICAL PROD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Candida vaginitis is fungal vaginitis, which is a common gynecological disease. At present, it is mainly treated with antibiotics in clinical practice, but long-term use of antibiotics can easily lead to recurrence of the disease

Method used

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  • Complex microbial inoculant for regulating gynecological micro-ecological balance
  • Complex microbial inoculant for regulating gynecological micro-ecological balance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1. Activated bacteria:

[0032] Lactobacillus crispatus CGMCC No.6364: Streak Lactobacillus crispatus CGMCC No.6364 into the MRS solid medium and culture it anaerobically at 37°C for 24 hours. Pick a single colony with good growth in the plate and inoculate it into MRS liquid medium for activation, and culture it anaerobically to 0.5*10 8 cfu / ml Lactobacillus crispatus CGMCC No.6364 seed solution.

[0033] Lactobacillus salivarius SIL1: The method of activating the strain is the same as above. The final configuration is 0.5*10 8 cfu / ml Lactobacillus salivarius curly seed solution.

[0034] 2. Configure liquid

[0035] Cycloastragenol solution, the concentration is 2mg / ml, 2-8 ℃, protected from light, sealed and stored at low temperature.

[0036] 3. Modeling of KM mice

[0037] Modeling of bacterial vaginosis in mice: Three kinds of miscellaneous bacteria were injected into the vagina of mice to produce vaginal inflammatory diseases. In this test, 0.3 mL of mixed...

Embodiment 2

[0058] Each group of mice in Example 1 was treated to detect the vaginal flora after treatment on the 8th day:

[0059] Detection of vaginal flora in mice: Take 100ul of vaginal douching solution under sterile conditions, mix it with normal saline, and then perform 10-fold serial dilutions in sequence, and select 10 times from the original solution. 3 、10 4 、10 5 、10 6 Dilution, were dropped in MRS, EMB, SP, LB medium.

[0060] Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa were cultured aerobically at 37°C, and Lactobacillus were cultured anaerobically for 48 hours, and the number of viable bacteria per milliliter of vaginal secretions (cfu / ml) was calculated by counting the colonies , after taking the logarithm for statistical processing.

[0061] Statistical analysis was carried out using SPSS16.0, and the results of measurement data were expressed as mean ± standard deviation, using t-test between groups. The results are shown in Table 2.

[0062]...

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Abstract

The invention discloses a complex microbial inoculant for regulating gynecological micro-ecological balance. The complex microbial inoculant is prepared from lactobacillus crispatus, lactobacillus salivarius SIL1 (Lactobacillus salivarius SIL1) and other acceptable drug excipients. The invention further discloses a preparation method of the complex microbial inoculant. The preservation number of the Lactobacillus crispatus strain is CGMCC No. 6364, and the preservation number of the lactobacillus salivarius SIL1 is CCTCC M2010281. The complex microbial inoculant can be prepared into oral liquid or fermented food, preferably capsules, bacterial powder, milk tablets, fermented food and the like. Preferably, the complex microbial inoculant also comprises cycloastragenol.

Description

technical field [0001] The invention relates to a compound microbial agent for regulating the micro-ecological balance of gynecology, belonging to the technical field of microbes. Background technique [0002] Vaginal dysbiosis can cause various gynecological inflammations, including vaginitis, (acute and chronic) cervicitis, cervical diseases, endometritis, uterine body inflammation, and annex inflammation. [0003] Antibiotics and other traditional bactericidal products, while regulating the exogenous infection of the female reproductive system and killing harmful bacteria, also kill the normal beneficial flora in the body. The imbalance of flora in the vagina leads to endogenous infections or opportunistic infections of the reproductive tract , bringing drug resistance of pathogenic bacteria, high disease recurrence rate, double infection and toxic reaction. Microecological therapy of gynecological microecological balance probiotic probiotic therapy instead of antibacter...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/747A61P15/02A61P31/02A61P31/04A23L33/105A23L33/135A23C9/18A23C9/152A61K31/58
CPCA61K35/747A61K31/58A61P15/02A61P31/02A61P31/04A23L33/105A23L33/135A23C9/18A23C9/152A23V2002/00A23C2240/15A23V2400/133A23V2400/181A61K2300/00A23V2200/30A23V2250/21
Inventor 赵一鸣汪伯良
Owner KUIMING JIAJIANING BIOLOGICAL PROD CO LTD
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