Porcine gatb gene as a molecular marker related to piglet mortality and its application
A technology for mortality and piglets, applied in the direction of recombinant DNA technology, microbial measurement/testing, DNA/RNA fragments, etc., can solve problems that need to be studied
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[0039] In this example, refer to figure 1 , screened for molecular markers associated with piglet mortality by the following methods.
[0040] 1. Pig data collection
[0041] (1) Collection of phenotypic data
[0042] The pigs used in the present invention to record phenotypic data are 6073 healthy sows from Guangxi Yangxiang Co., Ltd., and the breeds include: Dabai, Landrace and Duroc. The pedigree information used in this study included the pedigree information of these 6,073 sows, involving 35,313 individuals. Data included total litter size, total deaths, stillbirths, and mummies. Among them, the total number of deaths is equal to the number of stillbirths plus the number of mummies. The mortality trait studied in the present invention is the percentage of total deaths to total litter size.
[0043] (2) Genetic data collection
[0044] Pig ear tissue was used for DNA extraction, and all samples were stored in a -20°C freezer until then. DNA was extracted using the k...
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