Unlock instant, AI-driven research and patent intelligence for your innovation.

A method for extracting black soldier fly antimicrobial peptide and its application in the preparation of fertilizers against tobacco diseases

The technology of the antibacterial peptide of a soldier flies and the black soldier flies, which is applied to the application of fertilizers, and the field of extraction of the antibacterial peptides of the black soldier flies, can solve the problems that the antibacterial peptides of the black soldier flies are not used for the preparation of tobacco disease-resistant fertilizers, and the antibacterial peptides of the black soldier flies are few, and the like. Improve agronomic traits, good control effect, high content effect

Active Publication Date: 2022-03-08
松颉环保科技(深圳)有限公司
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there are few reports on the extraction and application of antimicrobial peptides from black soldier flies, and there is no report on the use of antimicrobial peptides from black soldier flies in the preparation of tobacco disease-resistant fertilizers

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for extracting black soldier fly antimicrobial peptide and its application in the preparation of fertilizers against tobacco diseases
  • A method for extracting black soldier fly antimicrobial peptide and its application in the preparation of fertilizers against tobacco diseases
  • A method for extracting black soldier fly antimicrobial peptide and its application in the preparation of fertilizers against tobacco diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] This embodiment provides a method for extracting black soldier fly antimicrobial peptides, comprising the following steps:

[0038] S1, preparation of R. solanacearum bacterium liquid: inoculate R. solanacearum bacteria in NA liquid medium (5g of peptone, 3g of beef extract, 1.5g of sodium chloride, 1L of deionized water), place at 30°C, 185r Cultivate in the dark for 54 hours under the condition of 1 / min, filter and dilute with sterilized water until the number of effective viable bacteria is 3.5×10 10 Bacterial solution of each / mL;

[0039] Preparation of tobacco wildfire bacteria liquid: Inoculate tobacco wildfire bacteria in NA liquid medium, culture in the dark at 28°C and 215r / min for 30h, filter and dilute with sterilized water until the number of effective viable bacteria is 3.5× 10 10 Bacterial solution of each / mL;

[0040] Preparation of Xanthomonas tabacum bacterial liquid: Inoculate Xanthomonas nicotiana into NA liquid medium, culture in the dark at 26°C ...

Embodiment 2

[0046] This embodiment provides a method for extracting black soldier fly antimicrobial peptides, comprising the following steps:

[0047] S1, preparation of R. solanacearum bacterium liquid: Inoculate R. solanacearum bacteria in NA liquid medium (5g of peptone, 3g of beef extract, 1.5g of sodium chloride, 1L of deionized water), place at 30°C, 170r Cultivate in the dark for 48 hours under the condition of 1 / min, and dilute with sterilized water after filtering until the number of effective viable bacteria is 2×10 10 Bacterial solution of each / mL;

[0048] Preparation of tobacco wildfire bacteria liquid: inoculate tobacco wildfire bacteria in NA liquid medium, culture in the dark at 28°C and 200r / min for 24 hours, filter and dilute with sterilized water until the number of effective viable bacteria is 2× 10 10 Bacterial solution of each / mL;

[0049] Preparation of Xanthomonas tabacum bacterial liquid: inoculate Xanthomonas nicotiana into NA liquid medium, culture at 26°C an...

Embodiment 3

[0055] This embodiment provides a method for extracting black soldier fly antimicrobial peptides, comprising the following steps:

[0056] S1, preparation of R. solanacearum bacterium liquid: Inoculate R. solanacearum bacteria in NA liquid medium (5g of peptone, 3g of beef extract, 1.5g of sodium chloride, 1L of deionized water), place at 30°C, 200r Cultivate in the dark for 60 hours under the condition of / min, and dilute with sterilized water after filtration until the number of effective viable bacteria is 5×10 10 Bacterial solution of each / mL;

[0057] Preparation of tobacco wildfire bacteria liquid: inoculate tobacco wildfire bacteria in NA liquid medium, culture at 28°C and 230r / min in the dark for 36h, filter and dilute with sterilized water until the number of effective viable bacteria is 5× 10 10 Bacterial solution of each / mL;

[0058] Preparation of Xanthomonas tabacum bacterial liquid: inoculate Xanthomonas nicotiana into NA liquid medium, culture at 26°C and 200...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the field of fertilizer preparation, and specifically relates to an extraction method of a black soldier fly antibacterial peptide and its application in the preparation of fertilizers resistant to tobacco diseases. In order to use the black soldier fly antibacterial peptides to prepare a fertilizer resistant to tobacco diseases, the present invention Using the composite bacterial liquid composed of R. solanacearum bacterial liquid, tobacco wildfire bacterial liquid and tobacco angular leaf spot bacterial liquid as the induction liquid, the black soldier fly was induced to produce a large amount of antimicrobial peptides under acupuncture soaking treatment, and prepared by enzymatic hydrolysis Obtained black soldier fly antimicrobial peptide, the gained black soldier fly antimicrobial peptide not only has high content, but also has high activity, and has better inhibitory effect on R. solanacearum, tobacco wildfire bacteria and tobacco leaf spot; Soldier fly antimicrobial peptides were used to prepare a kind of anti-tobacco disease foliar fertilizer. The foliar fertilizer showed good control effects on tobacco bacterial angular leaf spot, tobacco bacterial wilt and tobacco wildfire disease; and could improve the agronomic characteristics of tobacco And quality, improve its economic value.

Description

technical field [0001] The invention belongs to the field of fertilizer preparation, and in particular relates to a method for extracting black soldier fly antibacterial peptide and its application in preparing fertilizer for resisting tobacco diseases. Background technique [0002] Tobacco (Nicotiana tabacum) belongs to Solanaceae (Solanaceae) Nicotiana (Nicotiana), is an annual herb. my country is a big tobacco producing country in the world today, and the total output and sales of tobacco leaves account for about 30% of the world. Tobacco is an important economic plant in my country, and tobacco tax accounts for 1 / 10 of my country's fiscal revenue. Tobacco is one of the crops most affected by diseases, and there are many kinds of diseases and complex symptoms. Common tobacco diseases include tobacco bacterial wilt, tobacco wildfire disease and tobacco angular leaf spot, etc. The hazards of tobacco diseases not only cause economic losses, but also cause great harm to the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C12N1/20C05G3/60C12R1/38C12R1/01
CPCC12P21/06C12N1/20C05G3/00C05B7/00C05D9/02C05F11/00
Inventor 孟杰
Owner 松颉环保科技(深圳)有限公司