Sterilization preparation and sterilization method

A disinfection method and preparation technology, applied in the direction of disinfectants, botany equipment and methods, chemicals for biological control, etc., can solve problems such as difficult prevention and control

Active Publication Date: 2020-06-26
MITSUBISHI CHEM CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, oocysts are very resistant to environmental conditions, chemicals, etc., and it is very difficult to prevent and treat them with common disinfectants used for the prevention and treatment of bacteria and viruses.

Method used

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  • Sterilization preparation and sterilization method
  • Sterilization preparation and sterilization method
  • Sterilization preparation and sterilization method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0130] In Example 1, the sporulation inhibiting effect of allyl isothiocyanate on coccidia oocysts was verified.

[0131] Chickens were infected with spores of a field strain of Eimeria tenella (scientific name "Eimeria tenella") to form oocysts, and their feces were recovered on the 7th day after infection, and the unsporulated oocysts in the feces were separated and purified, and used as test material.

[0132] As a test substance, Wasaol (registered trademark, manufactured by Mitsubishi Chemical Foods Co., Ltd., powder preparation containing 10% by weight of allyl isothiocyanate, the same below) was dissolved in pure water to prepare a 5 mg / mL Wasaol solution. Add 1 mL (1 / 10 amount) of unsporulated oocyst floating liquid (number of oocysts: 2.1×10 5 per mL), sealed, and sensitized at 25°C for 1 hour.

[0133] After the sensitization, centrifuge (2000rpm, 5 minutes) to remove the supernatant, wash four times with 0.5% neutral lotion (2,000rpm, 5 minutes), remove the supern...

Embodiment 2

[0144] In Example 2, while examining the effective concentration of allyl isothiocyanate for the sporulation inhibitory effect, it was verified whether the sporulation inhibitory effect is maintained when it is used in combination with other disinfectants.

[0145] As a test substance for studying the effective concentration, 1 mg / mL, 0.75 mg / mL or 0.5 mg / mL wasaol solution was prepared in the same procedure as in Example 1.

[0146] As a test substance mixed with other disinfectants, a mixed solution of Wasaurol and Cryakil (Wasaurol 1 mg / mL, 0.75 mg / mL, or 0.5 mg / mL, and Cryakil 2 μL / mL) was prepared. In addition, CLIAKIL (registered trademark, manufactured by Tamura Pharmaceutical Co., Ltd.) is a disinfectant containing 10 g of didecyldimethylammonium chloride per 100 mL, and has a disinfectant effect on a wide range of pathogens other than coccidia such as viruses, bacteria, and molds.

[0147] In this embodiment, according to the dose of the preparation, the final concent...

Embodiment 3

[0155] In Example 3, the effect of allyl isothiocyanate on inhibiting sporulation was verified by using oocysts collected from cow feces suffering from coccidiosis as test materials.

[0156] Feces were collected from a cattle individual with diarrhea due to coccidiosis, and after being frozen for 3 days, oocysts that did not form spores were separated and purified from the feces by sucrose flotation method, and used as test materials. The results of microscopic observation showed that the isolated oocysts were all coccidia of the genus Eimeria in terms of their morphology, and there were at least three kinds of mixed infection.

[0157] As a test substance, 10 mg / mL or 5 mg / mL Wasaol solution was prepared in the same procedure as in Example 1, and 40 μL (1 / 10 amount) of unsporulated oocyst floating liquid (egg Number of capsules: 2.0×10 4 per mL), sealed, and sensitized at 25°C for 1 hour.

[0158] After the sensitization, the supernatant was removed by centrifugation (2,00...

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PUM

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Abstract

A coccidia sterilization preparation containing as an active ingredient the compound represented by general formula (I). The compound has a high spore-formation inhibition effect on oocysts of coccidia, and therefore is effective for the extermination of coccidia, and is effective for coccidia sterilization in rearing facilities / rearing rooms such as livestock barns, chicken coops, etc., for animals that are hosts. (In formula (I), R1 is a C2-8 alkenyl group, etc.)

Description

technical field [0001] The present invention relates to a preparation for disinfecting coccidia containing an isothiocyanate compound or the like as an active ingredient and a disinfection method. Background technique [0002] Coccidia are protozoa belonging to the subphylum Apizota of the kingdom Protozoa, and include many protozoa that become pathogens of diseases such as humans, domestic animals, and poultry. There are mainly protozoa such as Eimeria (scientific name "Eimeria"), Isospora (scientific name "Isospora") and Cryptosporidium (scientific name "Crypptosporidium") of the family Aimeriaceae Included in Coccidia, in addition, Sarcocystis (scientific name "Sarcocystis"), Toxoplasma (scientific name "ToxopPlasma"), Besnoitia, Harmon ( Protozoa such as Hammondia) are also classified as coccidia. [0003] Generally, coccidia develop in the following living environment. [0004] First, coccidia oocysts are excreted from the host. The single-celled oocyst immediately ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N47/46A01N25/02A01N33/12A01P1/00
CPCA01N47/46A01N33/12A01N31/08A61K31/26A61K31/14A01N29/04A61K2300/00A01N33/08
Inventor 上村良二大川和久
Owner MITSUBISHI CHEM CORP
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