Construction methods of multicomponent nanoparticle clusters
A nanoparticle and construction method technology, applied in the field of nanoparticle clusters, can solve problems such as singleness, and achieve the effect of improving controllability and facilitating subsequent purification
Active Publication Date: 2021-11-19
SHENZHEN INST OF ADVANCED TECH
View PDF4 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
[0005] The purpose of the present invention is to provide a method for constructing multi-component nanoparticle clusters, aiming to solve the problem that the prior art can only construct nanoparticle clusters with a single component
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0016] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present invention clearer, the present invention will be further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.
[0017] In the description of the present invention, it should be understood that the terms "first" and "second" are used for description purposes only, and cannot be interpreted as indicating or implying relative importance or implicitly indicating the quantity of indicated technical features. Thus, a feature defined as "first" and "second" may explicitly or implicitly include one or more of these features. In the description of the present invention, "plurality" means two or more, unless otherwise specifically defined.
[0018] to combine Figure 1-4 An embodiment of the present inventio...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The present invention provides a method for constructing the multi-component nanoparticle cluster, comprising the following steps: respectively providing N kinds of zinc finger proteins, N kinds of protein tags and M kinds of nanoparticles; combining one kind of the nanoparticle with at least one The above protein tags are cross-linked, and one protein tag only cross-links one kind of nanoparticle to obtain N kinds of nanoparticle / protein tag complexes; according to the unique pairing relationship, one kind of the zinc finger protein and one ligand Cross-linking to obtain N kinds of zinc finger protein / ligand complexes; providing nucleotide sequences, and complexing the nucleotide sequences with the N kinds of nanoparticle / protein label complexes and N kinds of zinc finger protein / ligands The body is mixed and incubated to form a connection system of nucleotide sequence-zinc finger protein-protein label-nanoparticles, and the obtained nanoparticle clusters are collected after separation, wherein the bases in the nucleotide sequence are in accordance with the preset nanoparticle Arrangement order setting of zinc finger proteins in granule clusters.
Description
technical field [0001] The invention belongs to the technical field of nanoparticle clusters, in particular to a method for constructing multicomponent nanoparticle clusters. Background technique [0002] Nanoparticle clusters are a specific composition or arrangement of nanoparticles formed through special interactions, which can better integrate and enhance the excellent properties of nanoparticles in optics, electricity, and magnetism, and then show better performance than simple aggregation of nanoparticles. nature. Especially because nanoparticle clusters can be modified with more recognition units, they have obvious advantages in targeted positioning and rapid enrichment. In addition, their good structural stability and low blood diffusion can effectively improve the signal-to-noise ratio. , biomarkers, medical imaging and other fields have a wide range of application value. [0003] At present, nanoparticle clusters are mostly prepared by using small molecules as me...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/00
CPCC07K14/00
Inventor 金宗文罗擎颖卫小元袁静赵江林
Owner SHENZHEN INST OF ADVANCED TECH