Subunit H protein of peste des petits ruminants virus and preparation method and application of subunit H protein

A technology of Peste des petits ruminants and subunits, applied in botany equipment and methods, biochemical equipment and methods, antisense single-stranded RNA viruses, etc. Stability H protein and other issues

Active Publication Date: 2020-07-07
NOVO BIOTECH CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, subunit vaccines also have obvious shortcomings: high production costs and limited applications
[0007] However, when using engineered cells to express H protein, if the coding gene sequence of H protein is no

Method used

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  • Subunit H protein of peste des petits ruminants virus and preparation method and application of subunit H protein
  • Subunit H protein of peste des petits ruminants virus and preparation method and application of subunit H protein
  • Subunit H protein of peste des petits ruminants virus and preparation method and application of subunit H protein

Examples

Experimental program
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Effect test

Embodiment 1

[0053] 1a: Structural analysis and optimization of the H protein of Peste des petits ruminants virus was carried out to construct the coding gene sequence of the subunit H protein of Peste des petits ruminants virus.

[0054] By analyzing the H protein sequence of Peste des petits ruminants virus (GenBank: KM091959.1), it was found that the genome sequence was 7332-9661bp, encoding the H protein sequence of Peste des petits ruminants. Further analysis 1M-35P is the intracellular region of H protein, 36Y-58I is the transmembrane region of H protein, and 59R-609V is the extracellular region of H protein. Combined with the experience of research on the expression of viral envelope proteins, the extracellular region of the H protein expressed by CHO-K1 cells was selected as the immunogenic protein of the present invention, that is, the amino acid sequence of 59R-609V, and the amino acid sequence is a known fragment, which can be obtained by Obtained by NBCI inquiry.

[0055] Howe...

Embodiment 2

[0241] Example 2: The difference from Example 1 is that in this example, codon optimization is not performed on the coding gene sequence of the subunit H protein, that is, the coding gene sequence of the subunit H protein is shown in SEQ ID NO.2 .

Embodiment 3

[0242] Example 3: The difference from Example 1 is that in this example, codon optimization is performed on the coding gene sequence of the subunit H protein, and its specific coding gene sequence is shown in SEQ ID NO.4.

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Abstract

The present invention belongs to the technical field of animal vaccines and veterinary biological products, and particularly relates to a subunit H protein of a peste des petits ruminants virus and apreparation method and application of the subunit H protein. The subunit H protein is a truncated head functional protein of an H protein of the peste des petits ruminants virus, amino acid sequencesof the subunit H protein are an amino acid sequence (1) as shown as SEQ ID NO.1 and a derived amino acid sequence (2) having the immunogenicity and obtained by performing substitution, deletion or addition of multiple amino acids on the sequence SEQ ID NO.1, and the derived amino acid sequence and the sequence SEQ ID NO.1 have the sequence identity of 80-100%. The subunit H protein is prepared mainly by constructing recombinant plasmids, transfecting cell strains with the recombinant plasmids, screening the high-expression cell strains and purifying the subunit H protein of the peste des petits ruminants virus, can be better suitable for subunit vaccines or diagnostic reagents of peste des petits ruminants viruses, and has the characteristics of efficient secretory expression, high proteinpurity, easy purification, low production cost, high safety performance and the like.

Description

technical field [0001] The invention belongs to the technical field of animal vaccines and veterinary biological products, and in particular relates to subunit H protein of Peste des Petits Ruminants virus and its preparation method and application. Background technique [0002] Peste des Petits of Ruminats (PPR) is a highly contagious viral disease caused by Peste des Petits of Ruminats Virus (PPRV). The World Organization for Animal Health (OIE) classifies it as a Class A infectious disease, and my country also classifies it as a Class I epidemic disease. PPRV mainly infects sheep, especially goats are highly susceptible. Once infected sheep will be characterized by sudden fever, depression, oral ulcers, cough, eye and nose discharge, and diarrhea. The morbidity and mortality can reach 100%, causing huge economic losses and seriously affecting the development of the sheep industry. [0003] At present, there is no effective treatment for the disease, mainly relying on va...

Claims

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Application Information

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IPC IPC(8): C07K14/115C12N15/45C12N15/85A61K39/155A61P31/14
CPCC07K14/005C12N15/85A61K39/12A61P31/14C12N2760/18422C12N2760/18434C12N2800/107C12N2800/22A61K2039/552
Inventor 钱泓吴有强卞广林张强徐玉兰吴素芳车影
Owner NOVO BIOTECH CORP
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