Subunit H protein of peste des petits ruminants virus and preparation method and application of subunit H protein

Abstract

The present invention belongs to the technical field of animal vaccines and veterinary biological products, and particularly relates to a subunit H protein of a peste des petits ruminants virus and apreparation method and application of the subunit H protein. The subunit H protein is a truncated head functional protein of an H protein of the peste des petits ruminants virus, amino acid sequencesof the subunit H protein are an amino acid sequence (1) as shown as SEQ ID NO.1 and a derived amino acid sequence (2) having the immunogenicity and obtained by performing substitution, deletion or addition of multiple amino acids on the sequence SEQ ID NO.1, and the derived amino acid sequence and the sequence SEQ ID NO.1 have the sequence identity of 80-100%. The subunit H protein is prepared mainly by constructing recombinant plasmids, transfecting cell strains with the recombinant plasmids, screening the high-expression cell strains and purifying the subunit H protein of the peste des petits ruminants virus, can be better suitable for subunit vaccines or diagnostic reagents of peste des petits ruminants viruses, and has the characteristics of efficient secretory expression, high proteinpurity, easy purification, low production cost, high safety performance and the like.

Description

technical field [0001] The invention belongs to the technical field of animal vaccines and veterinary biological products, and in particular relates to subunit H protein of Peste des Petits Ruminants virus and its preparation method and application. Background technique [0002] Peste des Petits of Ruminats (PPR) is a highly contagious viral disease caused by Peste des Petits of Ruminats Virus (PPRV). The World Organization for Animal Health (OIE) classifies it as a Class A infectious disease, and my country also classifies it as a Class I epidemic disease. PPRV mainly infects sheep, especially goats are highly susceptible. Once infected sheep will be characterized by sudden fever, depression, oral ulcers, cough, eye and nose discharge, and diarrhea. The morbidity and mortality can reach 100%, causing huge economic losses and seriously affecting the development of the sheep industry. [0003] At present, there is no effective treatment for the disease, mainly relying on va...

AI Technical Summary

Problems solved by technology

However, subunit vaccines also have obvious shortcomings: high production costs and limited applications

[0007] However, when using engineered cells to express H protein, if the coding gene sequence of H protein is not optimized, the engineered cells basically do not express H protein, and it is difficult to obtain H protein with excellent immunogenicity and stability for small ruminant Prevention and control of zooepidemic virus

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