Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Aspergillus sepultus and application thereof

A technology of Aspergillus and seeds, applied in the field of microorganisms, can solve the problems of Aspergillus tubingensis CT1's phosphorus-solubilizing ability and inapplicability, and achieve the effects of increased content, enhanced utilization rate, and enhanced root system

Active Publication Date: 2020-07-10
MOON (GUANGZHOU) BIOTECH CO LTD
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] CN104651237A discloses a high-efficiency phosphorus-dissolving Aspergillus niger strain and its application. The Aspergillus niger can convert insoluble phosphorus (Ca 3 (PO 4 ) 2 ) is decomposed into soluble available phosphorus, so that the available phosphorus content in the medium is as high as 0.680mg / mL, and the phosphorus solution effect is remarkable; Improve the yield of planted crops, but the Aspergillus niger is not suitable for phosphorus solubilization in high-salt concentration soil
[0006] CN106222096A discloses a strain of Aspergillus tubingensis CT1 and its application in saline-alkali soil phosphorus solution. The Aspergillus tubingensis CT1 can effectively improve the utilization rate of soil phosphorus and promote crop yield increase, but the solution of Aspergillus tubingensis CT1 Phosphorus capacity decreases with increasing salt concentration

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Aspergillus sepultus and application thereof
  • Aspergillus sepultus and application thereof
  • Aspergillus sepultus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1 Acquisition and morphological identification of buried Aspergillus sepultus MN114011

[0049] (1) Strain acquisition

[0050] Aspergillus sepultus MN114011 was isolated from agricultural soil collected from a peanut planting site in Jiayi Town, Yanshan County, Wenshan Prefecture, Yunnan Province (23°43′17.07″, 104°02′5.77″).

[0051] The separation method is as follows:

[0052] Weigh 5g of soil, add 45mL of sterile water, shake with a vortex shaker for 5min, and then dilute to 10 -1 ~10 -5 , take 10 -3 、10 -4 and 10 -5 Three gradient bacterial solutions were spread on the flat plate, spread on the organophosphate solid medium, each coating was repeated three times, and cultured upside down in a constant temperature incubator at 28°C for 4 days, after 4 days, the circles with phosphate solution were picked out The strains were purified and stored in glycerol tubes for the next step of re-screening;

[0053] The formula of organic phosphorus solid medium...

Embodiment 2

[0058] Example 2 Molecular identification of buried Aspergillus sepultus MN114011

[0059] The strain was streaked on a plate to obtain a single colony, and the single colony was picked and placed in a shaker tube containing 2mL TSB, and placed at 28°C for shaking culture until turbid; Take 1mL of the bacterial solution in a centrifuge tube, centrifuge at 10000rpm for 3min, discard the supernatant, add 50 μL of sterile water, shake well, incubate at 95°C for 2 minutes, and place in a refrigerator at 0°C for later use;

[0060] Use a toothpick to pick a small amount of hyphae into 20 μL lysate, and lyse at 95°C for 30 minutes; pipette 2 μL lysate into the PCR system, which includes 12.5 μL premix TaqMaster Mix, 10 μL ddH 2 O, 0.7 μ L ITS4 (SEQ ID NO: 2), 0.7 μ L ITS5 (SEQ ID NO: 3), 0.6 μ L DMSO, carry out PCR amplification;

[0061] Take 5 μL of the amplification product, add 1 μL of loading buffer, mix well, perform agarose gel electrophoresis for 45 min, and detect the ampl...

Embodiment 3

[0065] Example 3 strain preservation

[0066] Based on the morphological observation and molecular identification results of Examples 1-2, the strain MN114011 belongs to Aspergillus sepultus, and Aspergillus sp. MN114011 was preserved in Guangdong Microbial Culture Collection Center (GDMCC) on May 14, 2019 , the address is Guangdong Institute of Microbiology, Building 59, Compound, No. 100 Xianlie Middle Road, Guangzhou City, postcode 510070, and the deposit number is GDMCC 60666.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
diameteraaaaaaaaaa
Login to View More

Abstract

The invention provides aspergillus sepultus and an application thereof. The aspergillus sepultus is named as Aspergillus sp.MN114011, and is preserved in Guangdong Province Microbiological Culture Collection Center, wherein the preservation number is GDMCC 60666, and the preservation date is 14 May 2019. The Aspergillus sepultus MN114011 has notable phosphorus dissolving effects for insoluble phosphorus sources of which the concentration is as high as 20%, the content of available phosphorus can be effectively increased, and the utilization rate of soil phosphorus is increased. Besides, the aspergillus sepultus MN114011 can promote yield promotion of crops, for corn crops treated with the aspergillus sepultus MN114011, the root systems are obviously strengthened, and the aspergillus sepultus MN114011 has notable effect of promoting growth of crops. The aspergillus sepultus MN114011 has wide application prospects in the respect of preparation of a phosphorus dissolving agent and a growth promoter.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and relates to a buried aspergillus and its application, in particular to a buried aspergillus and its application in soil phosphorus removal and plant growth promotion. Background technique [0002] As one of the three major nutritional elements, phosphorus plays an important role in participating in organ composition and promoting metabolic activities, and has a significant role in promoting the synthesis, decomposition and transportation of carbohydrates in crops. Yield and quality are particularly affected. [0003] Since the 1950s, due to the excessive application of chemical phosphate fertilizers, the storage and accumulation of insoluble phosphorus in the soil has reached 60 million tons, exceeding the total consumption of phosphate fertilizers in the country for 10 years. Saline-alkali land is widely distributed in my country, especially in the Yellow River Delta region. In these ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/34A01P21/00C09K17/14C12R1/66C09K101/00
CPCC12N1/14C09K17/14C09K2101/00C12R2001/66C12N1/145
Inventor 李得盛邹秋霞
Owner MOON (GUANGZHOU) BIOTECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products