A special phosphorus-dissolving bacterium and bacterial agent for rapeseed

A technology of phosphorus-dissolving bacteria and phosphorus-dissolving bacteria agent, which is applied in the field of microorganisms to achieve the effect of promoting plant growth, promoting growth and increasing yield

Active Publication Date: 2019-05-10
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no research and application of dedicated phosphate-dissolving bacterial strains with specific affinity for rapeseed

Method used

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  • A special phosphorus-dissolving bacterium and bacterial agent for rapeseed
  • A special phosphorus-dissolving bacterium and bacterial agent for rapeseed
  • A special phosphorus-dissolving bacterium and bacterial agent for rapeseed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Screening and Identification of Rapeseed Phosphorus-Solubilizing Bacteria

[0023] 1. Collect 4 g of young root system of healthy rapeseed plants growing normally in the soil, put the root system in 200 mL of sterile saline, add 100 μL of Tween 80, shake at 150 r / min for 1 hour, and use the 10-fold dilution method to prepare into 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -5 、10 -7 Gradient suspension, take 10 -4 、10 -5 、10 -5 、10 -7 100 μL of the diluted suspension was spread on the phosphate-dissolving bacteria medium plate, and cultured at 30°C for 60 hours; the strains with larger transparent circles were picked and purified by streaking on the phosphate-dissolving bacteria medium plate to obtain 31 strains of phosphate-dissolving bacteria.

[0024] 2. Prepare rapeseed lectin, and use rapeseed lectin to conduct affinity agglutination test on the 31 strains of phosphorus-dissolving bacteria obtained above, and obtain 18 strains of phosphorus-dissolving bacteria ...

Embodiment 2

[0027] Phosphorus-solubilizing bacteria dedicated to rape Labrys portucalensis ) Determination of phosphate-solubilizing activity.

[0028] 1. Bacteria solution preparation

[0029] Preparation and determination of phosphorus-dissolving activity liquid medium, the medium formula: sucrose 10g, NaCl 0.3g, MgSO 4 ·7H 2 O 0.3g, Ca 3 (PO 4 ) 2 5.0g, MnSO 4 4H 2 O 0.03g, agar 15~20g, KCl 0.3g, (NH 4 ) 2 SO 4 0.5gFeSO 4 ·7H 2 O 0.03g, distilled water 1000mL, pH 7.0~7.5. Take 90 mL of phosphorus-dissolving active medium and put it in a 250 mL Erlenmeyer flask, and insert a ring of YP206 strain lawn into each bottle, and the control group is not inoculated with bacteria, and each group has 3 parallels; 30°C, 120 r / min shaker culture After 45 hours, the YP206 strain bacterial liquid for measuring the phosphorus-dissolving activity was obtained.

[0030] 2. Activity Assay

[0031] The phosphorus-dissolving activity of YP206 strain was determined by molybdenum blue colori...

Embodiment 3

[0035] Preparation and Application of Phosphate-Solubilizing Liquid Bacteria Specially Used for Rapeseed

[0036] 1. Preparation of phosphorus-dissolving liquid bacterial agent for rapeseed

[0037] Pick one ring of bacteria from the slant of phosphate-dissolving bacteria strain YP206, inoculate it in a 500 mL triangular flask filled with 150 mL of sterile beef extract peptone liquid medium, shake at 30°C and 150 r / min for 38 hours, and then prepare Rapeseed Phosphorus Solubilizing Bacteria YP206 Liquid Inoculum.

[0038] 2. Rapeseed inoculation cultivation

[0039]Soak the rape seeds in distilled water at room temperature for 4-6 hours, and place them in an artificial climate box at 28°C with a light-to-dark ratio of 14:10 to accelerate germination until the rape seeds turn white. Take air-dried yellow cinnamon soil and put it in flower pots, 2.5 kg / pot, and plant 10-15 pregerminated Luba rape seeds in each pot; control group A is fertilized according to normal field cultiv...

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Abstract

The invention relates to a special phosphorus-dissolving bacterium for rape and its agent. A strain of phosphate-dissolving bacteria dedicated to rapeseed was named YP206, and the taxonomic name was Bacterium portugalus ( Labrys portucalensis ), preserved in China Typical Culture Collection Center on May 23, 2016, address: China.Wuhan.Wuhan University; the preservation number is CCTCC M 2016274; the bacterial agent was obtained by expanding the phosphorus-dissolving bacteria for rapeseed; the bacterial agent Used for seed dressing, or coating, or watering, or dipping roots to achieve effective inoculation, and provide high-concentration available phosphorus nutrition for rapeseed. The phosphorus-dissolving bacteria specially used for rapeseed of the present invention can undergo agglutination reaction with rapeseed lectin, so it has affinity with target crops, can settle and survive in the root of rapeseed for a long time, and play a role in promoting the growth of rapeseed, increasing the phosphorus content of plants, and increasing the stress resistance of rapeseed . The invention can reduce the amount of phosphate fertilizer used in rapeseed cultivation, improve the utilization rate of phosphate fertilizer, change the disadvantages of low specificity and inconspicuous use effect of existing biological fertilizers, and is beneficial to the development of ecological agriculture.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a phosphorus-dissolving bacterium dedicated to rapeseed and its application. Background technique [0002] Rapeseed is an important economic crop, and phosphorus affects the yield and quality of rapeseed. Phosphorus is an important constituent element of life and participates in various metabolic pathways, especially for rapeseed. Phosphorus participates in fat metabolism, promotes the flower bud differentiation of rapeseed, increases the amount of oil synthesis, improves the quality of rapeseed, increases the oil content of rapeseed; shortens the growth period of rapeseed, promotes the robust growth of plants; enhances the lodging resistance, cold resistance and drought resistance of rapeseed, and resists The fluctuation of soil pH can better enhance the ability of rape to resist various diseases and insect pests. Therefore, rapeseed cultivation requires a large a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C09K17/14A01N63/00A01P21/00C12R1/01C09K101/00
CPCA01N63/00C09K17/14C09K2101/00C12N1/20C12N1/205C12R2001/01
Inventor 周可金白莉敏唐欣昀朱英华朱宗河曹媛媛孙乐妮陈晓琳邓瑞宁
Owner ANHUI AGRICULTURAL UNIVERSITY
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