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Composite phosphorus solubilizing microbial agent and preparation method and application thereof

A compound phosphorus-dissolving bacteria agent and a technology of phosphorus-dissolving bacteria agent are applied in the field of microorganisms, which can solve the problems of lack of effective phosphorus resources, ecological environment impact, etc., achieve high-efficiency phosphorus-dissolving, reduce production costs, and strengthen the ability to produce organic acids and phosphatases. Effect

Active Publication Date: 2017-09-15
ANHUI AGRICULTURAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

[0002] Phosphorus is one of the essential nutrients for plant growth. It is very important for plant growth, but most of the organic phosphorus and inorganic phosphorus in the soil cannot be directly absorbed and utilized by plants.
74% of the cultivated land in my country is deficient in phosphorus, and more than 95% of the phosphorus in the soil is ineffective phosphorus, resulting in an extreme shortage of available phosphorus resources
The application of phosphorus fertilizer can provide phosphorus nutrients to plants, but the seasonal utilization rate of phosphorus fertilizer by plants is generally only 5% to 25%. Due to the long-term application of phosphorus fertilizer to cultivated land, most of the phosphorus fertilizer accumulates in the cultivated soil as an invalid state, which affects the ecological environment

Method used

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  • Composite phosphorus solubilizing microbial agent and preparation method and application thereof
  • Composite phosphorus solubilizing microbial agent and preparation method and application thereof
  • Composite phosphorus solubilizing microbial agent and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The screening of embodiment 1WS32 bacterial strain and P1616 bacterial strain

[0033] 29 strains of wheat rhizosphere phosphate-solubilizing bacteria with specific affinity with wheat were screened from the wheat rhizosphere by selection medium combined with lectin screening method; different phosphorus-dissolving medium were selected and Ca 3 (PO 4 ) 2 、FePO 4 , AlPO 4 , CaHPO 4 2H 2 Insoluble inorganic phosphorus such as O, and insoluble organic phosphorus such as lecithin and calcium phytate; the concentration of soluble phosphorus in the fermentation broth of each strain was determined by molybdenum antimony anti-colorimetric method, and the dissolution of 29 strains to different forms of insoluble phosphorus was determined. capacity, and it was determined that 5 strains had better bacteriophosphate-dissolving effects. These five strains of high-efficiency phosphorus-solubilizing wheat strains were mixed and cultured in pairs in different proportions, and the...

Embodiment 2

[0034] The phosphorus-dissolving ability of embodiment 2 compound phosphate-dissolving bacteria agent

[0035] Mix the WS32 and P1616 bacterial solutions according to the volume ratio of 1:0, 0:1, 2:1, 1:1, and 1:2 respectively, and the number of bacteria in the two bacterial solutions is 1×10 8 ~9×10 8 Within the range of CFU / mL, insert 3% inoculum into tricalcium phosphate medium, culture in Erlenmeyer flask, culture conditions are 160r / min, 28℃, culture for 9 days; use molybdenum antimony anti-colorimetric method to regularly measure the fermentation broth every day The concentration of soluble phosphorus in figure 1 as shown, figure 1 Among them, W:P means WS32:P1616, and the phosphorus-dissolving effect of the compound phosphate-solubilizing bacteria is greatly improved compared with the single strain.

Embodiment 3

[0036] Embodiment 3 compound phosphate-dissolving bacteria agent secretes organic acid and phosphatase ability

[0037] (1) The principle of phosphate-dissolving bacteria to decompose inorganic phosphorus is to secrete organic acid, dissolve insoluble inorganic phosphorus in soil and turn it into soluble phosphorus, which can be absorbed and utilized by plants.

[0038] Tricalcium phosphate medium was used to measure the types and concentrations of organic acids by high performance liquid chromatography. The liquid shake flask was cultured for 7 days, and 1 mL of the bacterial solution was taken, centrifuged, vacuum filtered through a 0.22 μm filter membrane, and then subjected to liquid chromatography. Separation conditions: the column is Hypersil C 18 (250nm×4.6mm i d, 5μm), the mobile phase is 0.02 mol / L of KH 2 PO 4 Buffer solution-methanol solution (99%:1%), adjust the pH to 2.60 with 1mol / L phosphoric acid, the ultraviolet detection wavelength is 210nm, the injection ...

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Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to a composite phosphorus solubilizing microbial agent. The composite phosphorus solubilizing microbial agent is prepared by mixing and fermenting a phosphorus solubilizing bacterium WS32 and a phosphorus solubilizing bacterium P1616, the phosphorus solubilizing bacterium WS32 is classified and named as Pseudomonas sp., and was deposited in the China Center for Type Culture Collection on Jun. 09, 2017 with the number of CCTCC NO: M 2017319; and the phosphorus solubilizing bacterium P1616 is classified and name as Bacillus sp., and was deposited in the China Center for Type Culture Collection on Jun. 02, 2017 with the number of CCTCC NO: M 2017298. The composite phosphorus solubilizing microbial agent has the beneficial effects that WS32 and P1616 has compatibility with wheat, WS32, P1616 and the wheat can be subjected to agglutination reaction, and WS32 and P1616 can settle down and survive in roots of the wheat for a long time, and have effect of promoting growth of the wheat; and the composite phosphorus solubilizing microbial agent has high ability of producing organic acid and phosphatase, and the phosphorus solubilizing and resolving effect is efficient.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a compound phosphate-dissolving bacterial agent and its preparation method and application. Background technique [0002] Phosphorus is one of the essential nutrients for plant growth, and it is very important for plant growth, but most of the organic phosphorus and inorganic phosphorus in the soil cannot be directly absorbed and utilized by plants. 74% of the cultivated land in my country is deficient in phosphorus, and more than 95% of the phosphorus in the soil is ineffective phosphorus, resulting in an extreme shortage of available phosphorus resources. The application of phosphorus fertilizer can provide phosphorus nutrients to plants, but the seasonal utilization rate of phosphorus fertilizer by plants is generally only 5% to 25%. Due to the long-term application of phosphorus fertilizer to cultivated land, most of the phosphorus fertilizer accumulates in the c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C09K17/14C12R1/38C12R1/07C09K101/00
CPCC09K17/14C09K2101/00C12N1/20
Inventor 曹媛媛赵为容唐欣昀陈晓琳孙乐妮邓瑞宁郭婷婷
Owner ANHUI AGRICULTURAL UNIVERSITY
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