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A Buried Aspergillus and its Application

A technology of Aspergillus and seeds, which is applied in the field of microorganisms, can solve the problems of inapplicable phosphorus solubilization and the reduction of phosphorus solubilization ability of Aspergillus tubingensis CT1, and achieve the effect of enhancing utilization rate, increasing content, and promoting crop production

Active Publication Date: 2022-03-04
MOON (GUANGZHOU) BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] CN104651237A discloses a high-efficiency phosphorus-dissolving Aspergillus niger strain and its application. The Aspergillus niger can convert insoluble phosphorus (Ca 3 (PO 4 ) 2 ) is decomposed into soluble available phosphorus, so that the available phosphorus content in the medium is as high as 0.680mg / mL, and the phosphorus solution effect is remarkable; Improve the yield of planted crops, but the Aspergillus niger is not suitable for phosphorus solubilization in high-salt concentration soil
[0006] CN106222096A discloses a strain of Aspergillus tubingensis CT1 and its application in saline-alkali soil phosphorus solution. The Aspergillus tubingensis CT1 can effectively improve the utilization rate of soil phosphorus and promote crop yield increase, but the solution of Aspergillus tubingensis CT1 Phosphorus capacity decreases with increasing salt concentration

Method used

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  • A Buried Aspergillus and its Application
  • A Buried Aspergillus and its Application
  • A Buried Aspergillus and its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1 Acquisition and morphological identification of buried Aspergillus sepultus MN114011

[0049] (1) Strain acquisition

[0050] Aspergillus sepultus MN114011 was isolated from agricultural soil collected from a peanut planting site in Jiayi Town, Yanshan County, Wenshan Prefecture, Yunnan Province (23°43′17.07″, 104°02′5.77″).

[0051] The separation method is as follows:

[0052] Weigh 5g of soil, add 45mL of sterile water, shake with a vortex shaker for 5min, and then dilute to 10 -1 ~10 -5 , take 10 -3 、10 -4 and 10 -5 Three gradient bacterial solutions were spread on the flat plate, spread on the organophosphate solid medium, each coating was repeated three times, and cultured upside down in a constant temperature incubator at 28°C for 4 days, after 4 days, the circles with phosphate solution were picked out The strains were purified and stored in glycerol tubes for the next step of re-screening;

[0053] The formula of organic phosphorus solid medium...

Embodiment 2

[0058] Example 2 Molecular identification of buried Aspergillus sepultus MN114011

[0059] The strain was streaked on a plate to obtain a single colony, and the single colony was picked and placed in a shaker tube containing 2mL TSB, and placed at 28°C for shaking culture until turbid; Take 1mL of the bacterial solution in a centrifuge tube, centrifuge at 10000rpm for 3min, discard the supernatant, add 50 μL of sterile water, shake well, incubate at 95°C for 2 minutes, and place in a refrigerator at 0°C for later use;

[0060] Use a toothpick to pick a small amount of hyphae into 20 μL lysate, and lyse at 95°C for 30 minutes; pipette 2 μL lysate into the PCR system, which includes 12.5 μL premix TaqMaster Mix, 10 μL ddH 2 O, 0.7 μ L ITS4 (SEQ ID NO: 2), 0.7 μ L ITS5 (SEQ ID NO: 3), 0.6 μ L DMSO, carry out PCR amplification;

[0061] Take 5 μL of the amplification product, add 1 μL of loading buffer, mix well, perform agarose gel electrophoresis for 45 min, and detect the ampl...

Embodiment 3

[0065] Example 3 strain preservation

[0066] Based on the morphological observation and molecular identification results of Examples 1-2, the strain MN114011 belongs to Aspergillus sepultus, and Aspergillus sp. MN114011 was preserved in Guangdong Microbial Culture Collection Center (GDMCC) on May 14, 2019 , the address is Guangdong Institute of Microbiology, Building 59, Compound, No. 100 Xianlie Middle Road, Guangzhou City, postcode 510070, and the deposit number is GDMCC 60666.

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Abstract

The invention provides a buried Aspergillus and its application. The buried Aspergillus is named Aspergillus sp. MN114011 and is preserved in the Guangdong Microbial Culture Collection Center with the preservation number GDMCC 60666 and the preservation date is May 14, 2019. The buried Aspergillus sepultus MN114011 of the present invention has a significant phosphorus-dissolving effect on insoluble phosphorus sources with a concentration as high as 20%, can effectively increase the content of available phosphorus, and enhance the utilization rate of soil phosphorus; at the same time, it can promote crop production. The root system of the maize crop is obviously strengthened, and has a remarkable effect of promoting crop growth; the method for preparing phosphorus-dissolving agent and growth-promoting agent has broad application prospects.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and relates to a buried aspergillus and its application, in particular to a buried aspergillus and its application in soil phosphorus removal and plant growth promotion. Background technique [0002] As one of the three major nutritional elements, phosphorus plays an important role in participating in organ composition and promoting metabolic activities, and has a significant role in promoting the synthesis, decomposition and transportation of carbohydrates in crops. Yield and quality are particularly affected. [0003] Since the 1950s, due to the excessive application of chemical phosphate fertilizers, the storage and accumulation of insoluble phosphorus in the soil has reached 60 million tons, exceeding the total consumption of phosphate fertilizers in the country for 10 years. Saline-alkali land is widely distributed in my country, especially in the Yellow River Delta region. In these ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/34A01P21/00C09K17/14C12R1/66C09K101/00
CPCC12N1/14C09K17/14C09K2101/00C12R2001/66C12N1/145
Inventor 李得盛邹秋霞
Owner MOON (GUANGZHOU) BIOTECH CO LTD
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