Kit for detecting prostate cancer and preparation method of kit

A prostate cancer and kit technology, which is applied in the field of technical prostate cancer detection, can solve the problems of poor specificity and sensitivity of prostate cancer diagnosis, and achieve the effects of reducing the pain of biopsy, improving the diagnosis rate and low cost.

Pending Publication Date: 2020-07-10
WANNAN MEDICAL COLLEGE
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Problems solved by technology

[0004] In view of this, the purpose of the present invention is to propose a test kit for detecting prostate cancer and its preparation method, in order to solve the poor specificity and sensitivity of clinical prostate cancer diagnosis in the current technology.

Method used

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  • Kit for detecting prostate cancer and preparation method of kit
  • Kit for detecting prostate cancer and preparation method of kit
  • Kit for detecting prostate cancer and preparation method of kit

Examples

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Embodiment Construction

[0057]For the following, through the description of the examples, the specific implementation of the present invention, such as the manufacturing process involved, the operation and use method, etc., will be described in further detail, so as to help those skilled in the art to have a more complete concept of the invention and the technical solution of the present invention. , accurate and in-depth understanding.

[0058] In order to solve the poor specificity and sensitivity of clinical prostate cancer diagnosis in the prior art, the present invention provides a preparation method of a test kit for detecting prostate cancer, comprising the following steps,

[0059] Primer design and synthesis: follow the principles of PCR primer design, refer to the DNA conserved region sequences of PCA3 gene, PSA gene, PSMA gene and TMPRSS2:ERG gene provided by Genebank, design a pair of outer chain primers respectively, and design according to the products amplified by the outer chain primer...

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Abstract

The invention discloses a kit for detecting prostate cancer and a preparation method of the kit. Through the combination of an optimization TMA technology and a nested PCR technology and by adopting the combined detection of four biomarkers of PCA3, PSMA, TMPRSS2-ERG and PSA, the sensitivity and specificity of early diagnosis of prostate cancer are improved, and the detection kit with low cost andrapid detection of multiple markers is developed. The gaps in domestic medical diagnostic technology is made up, the diagnosis rate of early prostate cancer is improved, and the pain of prostate cancer biopsy is reduced.

Description

technical field [0001] The invention relates to the technical field of detection of prostate cancer, in particular to a kit for detecting prostate cancer and a preparation method thereof. Background technique [0002] Prostate cancer (PCa) is a common malignant tumor of the genitourinary system in elderly men, and its morbidity and mortality rank second among male malignant tumors in many western countries. With the aging of the population in our society and the improvement of diagnostic and screening methods, the incidence of prostate cancer in my country is also increasing year by year, and the importance of prostate cancer research has become increasingly prominent. Compared with European and American countries, the mortality rate of prostate cancer in my country is higher, and the early diagnosis rate is lower. Therefore, the screening diagnosis of early prostate cancer is of great significance in men in our country. [0003] Prostate cancer has no clinical symptoms in...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12Q1/6848C12N15/11
CPCC12Q1/6886C12Q1/6848C12Q2600/158C12Q2549/119C12Q2531/113
Inventor 王宏婷许忠建陈升马跃新费永强张晓宇刘森宁贺良王思怡胡唐彬
Owner WANNAN MEDICAL COLLEGE
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