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Whole blood sample pretreatment device

A pretreatment device and whole blood sample technology, applied in the fields of medicine and biochemistry, can solve the problems of limited blood sample collection and long time consumption, and achieve the effects of good stability, high detection efficiency, and improved accuracy

Pending Publication Date: 2020-07-10
SUZHOU DIAGVITA BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Serum or plasma samples need to be obtained by centrifugation, which takes a long time; on the other hand, for infants and young children, most of them use peripheral blood sampling, and the amount of blood sample collection is limited, so it is impossible to obtain serum or plasma by centrifugation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Whole blood sample pretreatment device, which includes a pretreatment tube and a pretreatment diluent, the pretreatment diluent is contained in the pretreatment tube, and several stainless steel beads are arranged in the tube body, and the uniform mixing of the liquid in the pretreatment tube is accelerated by adding stainless steel beads The speed, pretreatment tube includes a tube body and a tube cap. The tube body is a cylindrical tube, and the bottom of the tube body is provided with an arc-shaped concave surface.

[0016] The pipe body is a pipe body made of polystyrene, polyethylene, polypropylene, polycarbonate or polyvinyl chloride.

[0017] The stainless steel bead is a stainless steel bead of 316, 340 or 420 stainless steel, the diameter of the stainless steel bead is 2-5 mm, and the number of the stainless steel bead is one or more.

[0018] Components of pretreatment diluents include buffers, surfactants, inorganic salts, stabilizers, and preservatives.

[...

Embodiment 2

[0025] On the basis of Example 1, the difference from Example 1 is that the components of the pretreatment diluent are different, and in the components of the pretreatment diluent, the buffer is 4-(2-hydroxyethyl)-1-piperazine Propanesulfonic acid-sodium hydroxide buffer solution (HEPPS-NaOH), its concentration range 100mmol / L, pH range is 8.0, surfactant is Tween 40, its concentration range is 0.3%, inorganic salt is sodium sulfate, its concentration The range is 2%, the stabilizer is glucose, and its concentration range is 2%, and the preservative is thimerosal, and its concentration range is 0.1%.

[0026] On the Mindray BS220 biochemical analyzer, load the C-reactive protein assay kit (latex-enhanced immunoturbidimetric method) to test the repeatability of a single test of the sample after hemolysis and the stability within 10 hours of hemolysis.

[0027]

[0028]

[0029] stability Low concentration whole blood (mg / L) High concentration whole blood (mg...

Embodiment 3

[0031] On the basis of Example 1, the difference from Example 1 is that the components of the pretreatment dilution are different, and in the components of the pretreatment dilution, the buffer is 3-(N-morpholino)propanesulfonic acid-hydrogen Sodium oxide buffer solution (MOPS-NaOH), its concentration range 200mmol / L, pH range is 8.0, surfactant is Tween 80, its concentration range is 0.5%, inorganic salt is ammonium chloride, its concentration range is 3% , the stabilizer is trehalose, and its concentration range is 3%, and the preservative is Proclin-300, and its concentration range is 0.15%.

[0032] On the Mindray BS220 biochemical analyzer, load the C-reactive protein assay kit (latex-enhanced immunoturbidimetric method) to test the repeatability of a single test of the sample after hemolysis and the stability within 10 hours of hemolysis.

[0033]

[0034]

[0035] stability Low concentration whole blood (mg / L) High concentration whole blood (mg / L) ...

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Abstract

The invention discloses a whole blood sample pretreatment device. The pretreatment device comprises a pretreatment pipe and a pretreatment diluent. The pretreatment diluent is accommodated in the pretreatment pipe. A plurality of stainless steel beads are arranged in the pipe body. The stainless steel beads are added to accelerate the uniform mixing speed of liquid in the pretreatment pipe. The pretreatment diluent comprises a buffer solution, a surfactant, inorganic salt, a stabilizer and a preservative. The pretreatment pipe comprises a pipe body and a pipe cap. The pipe body is a cylindrical tube, the bottom of the pipe body is provided with an arc-shaped concave surface, the pipe cap is arranged at a top opening of the pipe body, and the pipe cap is in sealed connection with the pipe body. The whole blood sample pretreatment device disclosed by the invention can be used for pretreating a collected whole blood sample without separating serum or plasma, the pretreated whole blood sample can be widely applied to detection of a full-automatic biochemical analyzer and a specific protein instrument, the detection efficiency is higher, and the stability is good. The stabilizer component in the pretreatment diluent is beneficial to the preservation of the to-be-detected substance in the sample, so that the detection accuracy is improved, and the repeatability is good.

Description

technical field [0001] The invention relates to the technical fields of medicine and biochemistry, in particular to a whole blood sample pretreatment device. Background technique [0002] At present, in clinical practice, many items, especially specific protein items, need to be tested with serum or plasma, and special serum blood collection tubes or anticoagulant blood collection tubes (EDTA dipotassium / disodium, citrate sodium, heparin). Serum or plasma samples need to be obtained by centrifugation, which takes a long time; on the other hand, for infants and young children, peripheral blood is mostly collected, and the amount of blood sample collection is limited, so it is impossible to obtain serum or plasma by centrifugation. Contents of the invention [0003] The purpose of the present invention is to aim at the clinical detection described in the background technology, which requires the use of serum or plasma for detection, and the collected blood needs to be centr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/38G01N33/531G01N35/00
CPCG01N1/38G01N33/531G01N35/00G01N2001/386
Inventor 赵年福张辉王明伟贾引军金君玉吴一凡
Owner SUZHOU DIAGVITA BIOTECH
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