Method for increasing fucoxanthin content of phaeodactylum tricornutum by photosynthetic accelerant

A technology of Phaeodactylum fucoxanthin and photosynthetic accelerator, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, and the addition of compounds to stimulate growth, etc., which can solve the problems of low extraction content, high breeding cost, and low content To achieve the effect of promoting rapid accumulation, optimizing technical effects, and improving cell growth

Active Publication Date: 2020-07-14
NINGBO UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Fucoxanthin is widely found in various algae, marine phytoplankton, aquatic shellfish and other animals and plants; the earliest substances used to extract fucoxanthin are mainly large algae, such as kelp, hijiki, etc., due to the high cost of breeding The development and utilization of fucoxanthin are limited by the low extraction content and other reasons; researchers at home and abroad have found that fucoxanthin is also contained in Phaeodactylum tricornutum, and has the advantages of fast growth and reproduction and easy artificial cultivation, but fucoxanthin The content of fucoxanthin in Phaeodactylum tricornutum is extremely low, resulting in extremely expensive fucoxanthin. Therefore, it is urgent to provide a method that can increase the content of fucoxanthin in Phaeodactylum tricornutum

Method used

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  • Method for increasing fucoxanthin content of phaeodactylum tricornutum by photosynthetic accelerant
  • Method for increasing fucoxanthin content of phaeodactylum tricornutum by photosynthetic accelerant

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Embodiment 1

[0049] Phaeodactylum tricornutum in logarithmic growth phase and well-grown in the laboratory of Ningbo University was inoculated into the improved F / 2 culture medium at a weight ratio of 1:2 for inoculation and culture, and the culture temperature was 20°C , the light intensity is 60μmol / (m 2 s), light and dark cycles are alternately cultivated, and each cycle is 12h. During the cultivation process, shake the algae operation from time to time, and shake the algae 5 times a day;

[0050] Get the Phaeodactylum tricornutum algae liquid of cultivation every day, measure its absorbance value with the UV-5200 type ultraviolet spectrophotometer that Shanghai Yuan Analysis Instrument Co., Ltd. produces, and according to the regression equation y=124.14x-9.5138 (R 2 =0.9983) and the measured absorbance value is converted into the number of cells, thereby determining the growth cycle of the cells;

[0051] When Phaeodactylum tricornutum was inoculated and cultivated for 4 days, when i...

Embodiment 2

[0053] Phaeodactylum tricornutum in logarithmic growth phase and well-grown in the laboratory of Ningbo University was inoculated into the improved F / 2 culture medium at a weight ratio of 1:2.5 for inoculation and culture, and the culture temperature was 21°C , the light intensity is 70μmol / (m 2 s), light and dark cycles are alternately cultivated, and each cycle is 12h. During the cultivation process, shake the algae operation from time to time, and shake the algae 6 times a day;

[0054] Get the Phaeodactylum tricornutum algae liquid of cultivation every day, measure its absorbance value with the UV-5200 type ultraviolet spectrophotometer that Shanghai Yuan Analysis Instrument Co., Ltd. produces, and according to the regression equation y=124.14x-9.5138 (R 2 =0.9983) and the measured absorbance value is converted into the number of cells, thereby determining the growth cycle of the cells;

[0055] When Phaeodactylum tricornutum was inoculated and cultivated for 4 days, when...

Embodiment 3

[0057] Phaeodactylum tricornutum in logarithmic growth phase and well-grown in the laboratory of Ningbo University was inoculated into the improved F / 2 culture medium at a weight ratio of 1:3 for inoculation and cultivation, and the cultivation temperature was 19°C , the light intensity is 80μmol / (m 2 s), light and dark cycles are alternately cultivated, each cycle is 12h, during the cultivation process, shake the algae operation from time to time, and shake the algae 8 times a day;

[0058] Get the Phaeodactylum tricornutum algae liquid of cultivation every day, measure its absorbance value with the UV-5200 type ultraviolet spectrophotometer that Shanghai Yuan Analysis Instrument Co., Ltd. produces, and according to the regression equation y=124.14x-9.5138 (R 2 =0.9983) and the measured absorbance value is converted into the number of cells, thereby determining the growth cycle of the cells;

[0059] When Phaeodactylum tricornutum was inoculated and cultivated for 4 days, wh...

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Abstract

The present invention belongs to the field of algal biotechnology, and particularly provides a method for increasing the fucoxanthin content of phaeodactylum tricornutum by a photosynthetic accelerant. The phaeodactylum tricornutum is cultured in a self-made culture medium, and when the phaeodactylum tricornutum is in a logarithmic growth phase, a photosynthetic induction factor (PIF) is added, sothat cell growth of the phaeodactylum tricornutum can be improved, fucoxanthin related gene expression can be quickly induced, and rapid accumulation of fucoxanthin is promoted. When the phaeodactylum tricornutum is cultured to be in the logarithmic growth phase, the PIF is added, so that the cell quantity of the phacodactylum tricornutum is increased by 17.7%, the fucoxanthin content is increased by 44%, and the problem of scarce fucoxanthin in the prior art is successfully solved.

Description

technical field [0001] The invention relates to the field of algae biotechnology, in particular to a method for increasing the content of fucoxanthin in Phaeodactylum tricornutum with a photosynthetic accelerator. Background technique [0002] Fucoxanthin is a natural pigment of xanthophylls in carotenoids. Studies have shown that fucoxanthin has a variety of health-promoting properties, such as anti-obesity, anti-diabetes, lowering blood pressure, anti-oxidation, anti- Cancer, neuroprotection, treatment of eye inflammation, etc., are widely used in the market as drugs, skin care and beauty products, and health care products; among them, the antioxidant properties of fucoxanthin are considered to be the main mechanism for exerting beneficial effects on health. [0003] Fucoxanthin is widely found in various algae, marine phytoplankton, aquatic shellfish and other animals and plants; the earliest substances used to extract fucoxanthin are mainly large algae, such as kelp, hij...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/38C12N1/12C12P17/02C12R1/89
CPCC12N1/38C12N1/12C12P17/02
Inventor 李申睿龚一富方清姝郑小恽韦凤娟王何瑜徐润洁
Owner NINGBO UNIV
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