A kind of breeding method of rice with low cadmium accumulation

A low-accumulation, rice technology, applied in the field of breeding of low-cadmium-accumulation rice, can solve the problem that materials cannot be used quickly, and achieve the effects of alleviating cadmium pollution, liberating labor, and keeping output unchanged.

Active Publication Date: 2021-07-23
HUNAN HYBRID RICE RES CENT +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the strict supervision of genetically modified rice in my country, these materials cannot be quickly used in production

Method used

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  • A kind of breeding method of rice with low cadmium accumulation
  • A kind of breeding method of rice with low cadmium accumulation
  • A kind of breeding method of rice with low cadmium accumulation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Breeding method of cadmium low accumulation rice, including the following steps:

[0059] (1) As a donor, 9311 rice is receptified as a donor.

[0060] (2) Hybridize the donor and receptor materials to obtain hybrid F1 generation seeds, and F1 generation seed self-giving F2 generation seed;

[0061] (3) Take detection using a specific Indel molecular marker in the F2 generation population, and the plants in which the homogenous OSNRAMP5 gene deletion is screened in the F2 group, that is, cadmium low accumulation rice material.

[0062] The method for detecting the specific Indel molecular marker is the same as in Example 1, and the specific Indel molecular marker includes a forward primer F (name P5-C8F) and reverse primer R (name P5-C8R):

[0063] P5-C8F: 5'-caatccaagacccgggggcatgat-3 '(as shown in SEQ ID NO.1);

[0064] P5-C8R: 5'-gcgccccataggattagttga-3 '(shown in SEQ ID No.2).

[0065] For the separation progeny generated in step (3), the specific Indel molecular marker...

Embodiment 2

[0070] Breeding method of cadmium low accumulation rice, including the following steps:

[0071] (1) As a donor, non-cadmium, low accumulation of rice varieties;

[0072] (2) Hybridize the donor and receptor material to obtain a hybrid F1 seed, F1 generation seed and receptor material return to obtain BC1F1 generation seed;

[0073](3) In the BC1F1 group, the specific Indel molecular marker is used to detect. In the BC1F1 population, the plants in which the hybrid OSNRAMP5 gene deletion is screened, and the resulting procedural group is detected by a specific Indel molecular mark. Screen A plant in which a homozygous OsnRamp5 gene is deleted, that is, cadmium low accumulation rice material.

[0074] The method for detecting the specific Indel molecular marker is the same as in Example 1, and the specific Indel molecular marker includes the following primer pairs:

[0075] P5-C8F: 5'-caatccaagacccgggggcatgat-3 '(as shown in SEQ ID NO.1);

[0076] P5-C8R: 5'-gcgccccataggattagttga-3 ...

Embodiment 3

[0082] Breeding method of cadmium low accumulation rice, including the following steps:

[0083] (1) As a donor, non-cadmium, low accumulation of rice varieties;

[0084] (2) Hybridize the donor and receptor material to obtain a hybrid F1 seed, F1 generation seed and receptor material return to obtain BC1F1 generation seed;

[0085] (3) In the BC1F1 group, the specific Indel molecular marker is detected. In the BC1F1 population, the plants in which the heterozygous OSNRAMP5 gene deletion is screened, and the receptor material is returned to the receptor material. The deletion of the transduction of OSNRAMP5 gene lacking plants, back-to-generation groups of retirement, and self-trails are detected by specific Indel molecular markers. Finally, the self-separation separation of plants in the deletion of homozygous OSNRAMP5 genes, that is, cadmium low accumulation rice material .

[0086] The method for detecting the specific Indel molecular marker is the same as in Example 1, and the...

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Abstract

The invention discloses a method for breeding rice with low cadmium accumulation. The existing low-cadmium-accumulation rice varieties Luohong 3A or Luohong 4A are used as donors, and non-cadmium-low-accumulation rice varieties are used as acceptors to carry out hybrid breeding of cadmium. Low accumulation rice material. In the breeding method of the present invention, the existing low-cadmium-accumulating rice variety is used as a donor, and the non-cadmium-low-accumulating rice variety is used as a recipient, and through methods such as conventional hybridization, self-crossing or backcrossing, specific InDel molecular markers are applied to the offspring population The low-cadmium-accumulation rice material with homozygous OsNRAMP5 gene deletion can be screened out, and the low-cadmium-accumulation rice material can be bred within two generations at the fastest. This method is simple and efficient, has high accuracy, short cycle time, and low cost. Transgenic means have effectively alleviated the current problem of cadmium pollution in rice.

Description

Technical field [0001] The present invention belongs to the field of crop genetic breeding, and involves a breeding method of cadmium low accumulation rice. Background technique [0002] Heavy metal element cadmium has a high toxicity to plants and humans, and its semi-life is decades. Cadmium in soil and waters mainly accumulate into the human body through the food chain, accumulating in the kidneys and continuously enriched in the human body. "Painful disease" produced in Japan in the early 20th century is caused by cadmium enrichment in the human body. Due to the long-term long-term intake of cadmium, even if the low level long-term intake will cause irreversible damage to the human body's respiratory system, and the cadmium has been scratched by WHO. [0003] Rice as one of the most important food crops in my country, which will be affected by non-essential elemental cadmium throughout the growth period, which will not only affect rice normally growth, but also seriously affe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H1/04A01H6/46C12Q1/6895
CPCA01H1/02A01H1/04A01H6/4636C12Q1/6895C12Q2600/13C12Q2600/156
Inventor 吕启明袁定阳朱仁山吴贤婷唐丽孙志忠黄志远何强
Owner HUNAN HYBRID RICE RES CENT
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