A kind of mofs type molecularly imprinted polymer and its preparation method, pesticide residue fluorescence detection method

A technology for pesticide residue and fluorescence detection, applied in fluorescence/phosphorescence, chemical instruments and methods, analytical materials, etc., can solve problems such as difficulty in meeting the needs of grassroots rapid screening and monitoring and early warning, single detection type, and difficulty in preparing biometric molecules.

Active Publication Date: 2022-02-01
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For example, the preparation of biorecognition molecules is difficult, the cycle is long, and the cost of detection is relatively high; it is easily interfered by complex matrices, has poor stability and repeatability, and the detection rate of false positives and false negatives is high; Monitoring and early warning needs

Method used

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  • A kind of mofs type molecularly imprinted polymer and its preparation method, pesticide residue fluorescence detection method
  • A kind of mofs type molecularly imprinted polymer and its preparation method, pesticide residue fluorescence detection method
  • A kind of mofs type molecularly imprinted polymer and its preparation method, pesticide residue fluorescence detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0091] This embodiment provides a method for fluorescent detection of pesticide residues, which includes the following steps:

[0092] S1: FeCl 3 ·6H 2 O, FeCl 2 4H 2 O and high-purity water are mixed according to a certain volume ratio and placed in a 250mL three-necked bottle for ultrasonic dispersion for 0.5h; magnetically stirred for 0.5-1h at a temperature of 50-100°C, and a certain volume of NH with a mass fraction of 28% is added 3 ·H 2 O, continue to react for 1 h under the protection of nitrogen; after magnetic separation, use absolute ethanol and pure water to alternately wash several times, and vacuum dry the obtained magnetic nanoparticles Fe 3 o 4 NPs; among them, FeCl 3 ·6H 2 O, FeCl 2 4H 2 The consumption ratio of O, high-purity water and ammoniacal liquor is 0.5g:0.15g:200mL:5mL; The used dehydrated alcohol and high-purity water volume ratio of each washing are 10mL:15-30mL;

[0093] S2: The magnetic nanoparticles Fe 3 o 4 Add NPs and high-purity wa...

Embodiment 2

[0099] It differs from Example 1 in that:

[0100] In step S1, FeCl 3 ·6H 2 O, FeCl 2 4H2 The consumption ratio of O, high-purity water and ammoniacal liquor is 0.6g:0.35g:200mL:10mL; The used dehydrated alcohol and high-purity water volume ratio of each washing are 10mL:25mL.

[0101] In step S2, the magnetic nanoparticles Fe 3 o 4 NPs, high-purity water, FeCl 3 ·6H 2 O, FeCl 3 ·6H 2 O aqueous solution, H 2 The dosage ratio of BDC and DMF is 0.2g:200mL:0.45g:80mL:0.174g:50mL.

[0102] In step S3, the magnetic MOFs composite Fe 3 o 4 - The dosages of MIL-Fe, methanol, acetamiprid molar mass, clothianidin molar mass, APTES, TEOS and acetic acid solution are 0.4g:10mL:0.15mM:0.15mM:0.2mL:2mL, respectively.

Embodiment 3

[0104] It differs from Example 1 in that:

[0105] In step S1, FeCl 3 ·6H 2 O, FeCl 2 4H 2 The consumption ratio of O, high-purity water and ammoniacal liquor is 0.75g:0.45g:200mL:15mL; The used dehydrated alcohol and high-purity water volume ratio of each washing are 10mL:30mL.

[0106] In step S2, the magnetic nanoparticles Fe 3 o 4 NPs, high-purity water, FeCl 3 ·6H 2 O, FeCl 3 ·6H 2 O aqueous solution, H 2 The dosage ratio of BDC and DMF is 0.3g:200mL:0.9g:120mL:0.498g:100mL.

[0107] In step S3, the magnetic MOFs composite Fe 3 o 4 - The dosages of MIL-Fe, methanol, acetamiprid molar mass, clothianidin molar mass, APTES, TEOS and acetic acid solution are 0.5g:10mL:0.2mM:0.2mM:0.4mL:4mL, respectively.

[0108] In conjunction with above-mentioned embodiment and accompanying drawing, refer to figure 1 , MIL-Fe is a blocky three-dimensional highly cross-linked polymer with a non-smooth surface and irregular corners; when Fe 3 o 4 Fe prepared after doping modif...

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Abstract

The invention discloses a MOFs type molecularly imprinted polymer, a preparation method thereof, and a fluorescence detection method for pesticide residues, and relates to the technical field of pesticide detection. In this method, MOFs are used as functional carriers, and the metal-organic framework / molecularly imprinted polymer (MOFs-type molecularly imprinted polymer Fe 3 o 4 ‑MIL‑Fe@MIPs), which not only has the advantages of adjustable pore size and high specific surface area, but also has the characteristics of rapid adsorption and high selective recognition of surface molecular imprinting, which can be combined with catalase catalytic and biomimetic recognition characteristics, Based on the relatively linear relationship between the concentration Log value of neonicotinoid pesticide residues and the fluorescence intensity of the solution, a rapid fluorescence detection method for neonicotinoid pesticide residues based on MOFs molecular imprinting was constructed to achieve rapid fluorescence detection of pesticide residues and effectively improve detection efficiency.

Description

technical field [0001] The invention relates to the technical field of pesticide detection, in particular to a MOFs type molecularly imprinted polymer, a preparation method thereof, and a fluorescence detection method for pesticide residues. Background technique [0002] There are currently 8 types of neonicotinoid pesticides that have been used on the market, namely imidacloprid, acetamiprid, dinotefuran, nitenpyram, clothianidin, thiacloprid, thiamethoxam, and piperacrid. Neonicotinoid pesticides have been widely used in vegetables in recent years because of their broad insecticidal spectrum and low interaction with traditional pesticides. With the sharp increase in usage, the risk of people being exposed to neonicotinoid pesticides through contaminated vegetables has also increased accordingly. The possible quality and safety issues of vegetable products caused by neonicotinoid pesticide residues have attracted more and more attention from consumers. At present, most of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08G83/00C08K3/22C08J9/26B01J20/26B01J20/30G01N21/64
CPCC08G83/008C08K3/22C08J9/26B01J20/268G01N21/643C08K2003/2275C08K2201/01
Inventor 刘广洋徐东辉高明坤李凌云黄晓冬许晓敏林桓张延国
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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