Quadruple inactivated vaccine and preparation method thereof

An inactivated vaccine and inactivated technology, applied in biochemical equipment and methods, vaccines, multivalent vaccines, etc., can solve the problems of poultry adenovirus farming losses and other issues

Inactive Publication Date: 2020-08-07
扬州优邦生物药品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As there is currently no clinically approved poultry adenovirus vaccine, the...

Method used

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  • Quadruple inactivated vaccine and preparation method thereof
  • Quadruple inactivated vaccine and preparation method thereof
  • Quadruple inactivated vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1 4

[0060] The antigen composition of embodiment 1 quadruple inactivated vaccine

[0061] The antigens of the quadruple inactivated vaccine are inactivated Newcastle disease La Sota virus strain, inactivated H9 subtype avian influenza TL18 strain, inactivated mutant bursal virus VP2 protein and inactivated group I avian adenovirus type 8b Hexon protein; the nucleotide sequence of the mutant strain bursal virus VP2 protein is as shown in SEQ ID NO.1, and the amino acid is as shown in SEQ ID NO.2; the nucleus of the I group 8b type avian adenovirus Hexon protein The nucleotide sequence is shown in SEQ ID NO.3, and the amino acid is shown in SEQ ID NO.4; the content of the chicken Newcastle disease La Sota virus strain is more than or equal to 10 8.5 EID 50 / 0.1mL; the content of the H9 subtype avian influenza TL18 strain ≥10 7.5 EID 50 / 0.1mL; the agar amplification titer of the VP2 protein content of the mutant strain bursal bursal virus ≥ 1:128; the agar amplification titer of ...

Embodiment 2 4

[0065] The preparation of embodiment 2 quadruple inactivated vaccine

[0066] S1. Isolation and identification of mutant bursal virus

[0067] Collect the bursa tissue of the diseased chicken, grind it, add double-antibody-containing PBS to dilute it into a suspension, add an equal volume of chloroform to the suspension, place it in a low temperature environment at 20 rpm for 24 hours, and take the supernatant after centrifugation at 3000 rpm Packed in ampoule vials for later use. The processed samples were inoculated into 10-day-old chicken embryos with the choriourin pathway (CAM), and incubated at 37°C. The chicken embryos that died within 24 hours were discarded, and the chicken embryos that died after 24 hours were placed at 4°C, the allantoic fluid was collected for hemagglutination test, and the embryo bodies and urea membranes were collected at the same time, which were aseptically ground and then packed in ampoules for later use. The total RNA of the culture was ext...

Embodiment 3

[0103] The safety test of embodiment 3 vaccines

[0104] According to the preparation method of the quadruple inactivated vaccine of Example 1, two batches of quadruple inactivated vaccines are prepared, and the batch numbers are respectively NABFs01 and NABFs02, and safety tests are carried out respectively:

[0105] (1) Single-dose one-time vaccination safety test

[0106] Get 10 days old SPF (no specific pathogen) chicken, the injection dosage of described quadruple inactivated vaccine is 0.3mL / , 10 / group, totally three groups, the first group injection batch number is the quadruple inactivated vaccine of NAIBFs01 , the second group was injected with quadruple inactivated vaccine with batch number NAIBFs02, and the third group was injected with 0.3 mL of normal saline in the neck of 10 animals;

[0107] Get the AA white-feathered broiler chicken (broiler for short) of 7 days of age simultaneously, the injection dosage of described quadruple inactivated vaccine is 0.3mL / , 1...

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Abstract

The invention provides a method for preparing a quadruple inactivated vaccine by adopting VP2 protein of a bursal disease virus variant FJ19 (with the preservation number of CGMCC NO: 19381), and a corresponding vaccine. Antigens of the vaccine are a newcastle disease La Sota virus strain, an avian influenza TL18 strain (with the preservation number of CGMCC NO: 19382), a VP2 protein of a bursal disease virus variant FJ19 and an I group 8b type avian adenovirus Hexon protein. The quadruple inactivated vaccine disclosed by the invention is high in VP2 protein and Hexon protein content, high inimmunogenicity and low in formaldehyde and endotoxin content, has a relatively good protection effect on the currently popular variant bursal disease virus FJ19 and the group I 8b type avian adenovirus, is good in safety performance and definite in immune effect, can resist infection of Newcastle disease virus, avian influenza virus, variant bursal disease virus and group I 8b type avian adenovirus to clinical chicken flocks at the same time, is small in immune dose and long in duration, can reach the immune duration of 5 months under the condition of only 0.3 mL once, and at least provides aprotection rate of 70% for the chicken flocks.

Description

technical field [0001] The invention belongs to the technical field of biological veterinary medicine, and in particular relates to a novel quadruple inactivated vaccine against Newcastle disease, infectious bursa, avian influenza and adenovirus and a preparation method thereof. Background technique [0002] Newcastle disease (ND) is an acute, febrile, septic and highly contagious infectious disease of poultry caused by Newcastle disease virus. It is characterized by high fever, dyspnea, diarrhea, nervous disturbance, and mucosal and serosal hemorrhage. It has a high morbidity and fatality rate and is one of the most serious severe infectious diseases that endanger the global poultry industry. OIE lists it as a class A disease, and it is one of the five first-class animal diseases stipulated in my country's "National Medium and Long-Term Animal Disease Prevention and Control Plan" for priority prevention and control. It is listed as a notifiable animal disease by the World...

Claims

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Application Information

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IPC IPC(8): A61K39/295A61K39/17A61K39/145A61K39/12A61K39/235A61P31/16A61P31/14A61P31/20
CPCA61K39/12A61K2039/5252A61K2039/552A61K2039/70A61P31/14A61P31/16A61P31/20C12N2710/10234C12N2720/10034C12N2760/16134C12N2760/18134
Inventor 杨振王建国李琛钱钟马玉峰李甜甜杨玉倩徐萍魏荣荣江媛范娟潘杰
Owner 扬州优邦生物药品有限公司
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