Method for improving immunohistochemical efficiency

Abstract

The invention discloses a method for improving immunohistochemical efficiency. The method comprises the following steps: 1, preparing slices; 2, performing dewaxing and hydrating; 3, performing antigen repair; 4, performing washing; 5, adding a primary antibody; 6, performing sealing; 7, adding a secondary antibody; 8, developing color; wherein all the steps are carried out in an electric field environment. The method is characterized in that the whole immunohistochemical process is carried out in an electric field environment; therefore, low-environmental load type trace liquid drop nondestructive stirring is realized, the use amount of the antibody is greatly reduced, and the cost is saved; more importantly, the reaction time of tissue immunodiagnosis can be greatly shortened; the reaction time of the tissue immunodiagnosis can be shortened from the current 177 min level to 19 min; in addition, the method can be used for nucleic acid rapid diagnosis, the reaction time can be shortened from the current 720 min level to 30 min, and the diagnosis efficiency can be substantially improved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for improving the efficiency of immunohistochemistry. Background technique [0002] Immunohistochemistry (IHC) is a chromogenic agent that labels antibodies through chemical reactions, and uses the principle of specific binding of antigens and antibodies to qualitatively and quantitatively study unknown antigens in tissues or cells. In recent years, with the development of immunohistochemical techniques, many difficult tumors have been clearly diagnosed. Immunohistochemistry plays a very important role in the diagnosis of tumor cells in clinical sections. Immunohistochemistry technology in my country was developed in the 1960s. However, there are still many technical bottlenecks in the clinical diagnosis of immunohistochemistry in my country at this stage. For example, the time spent by immunohistochemistry is very long. Usually, The immunodiagnostic reaction ne...

AI Technical Summary

Problems solved by technology

Immunohistochemistry technology in my country was developed in the 1960s. However, there are still many technical bottlenecks in the clinical diagnosis of immunohistochemistry in my country at this stage. For example, the time spent by immunohistochemistry is very long. Usually, The immunodiagnostic reaction needs to be overnight, and the time exceeds 24 hours, which cannot achieve the purpose of on-site rapid detection

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