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Preparation and application methods of triple composite deodorant

A technology of deodorant and bacterial strain, which is applied in the field of preparation of triple compound deodorant, can solve problems such as reducing odor, achieve the effects of reducing sulfide content, not polluting the environment, and high survival rate

Inactive Publication Date: 2020-08-25
南京师盛生态环境研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a preparation and application method of a triple compound deodorant to solve the problems of screening highly active and odor-reducing strains and their compounding, as well as the application of the above-mentioned strains in practice

Method used

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  • Preparation and application methods of triple composite deodorant

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] A preparation method of a triple compound deodorant. The triple compound deodorant is composed of Thiobacillus thifera, Rhodospirillum globosa, and plant lactic acid bacteria. The specific steps of preparation are as follows:

[0045] Step 1. Thiobacillus sphaericus was isolated from the black and odorous section of the Chuhe River in Liuhe District, Nanjing, and Rhodospirillum globosa and Lactobacillus plantarum were isolated from the bottom mud of Nanjing City Landscape River by our laboratory. Store in LB solid plates and store in a refrigerator at 4°C;

[0046] Step 2. Use an inoculation loop to pick up the three bacterial strains in step 1 and inoculate them into the liquid medium respectively, cultivate them on a shaking table at 30°C and 120rpm for 18 hours, and store them at 4°C until use.

[0047] Step 3, primary fermentation of bacterial strains: the three kinds of bacteria for use in step 2 are successively carried out primary fermentation and cultivation in ...

Embodiment 2

[0053] A preparation method of a triple compound deodorant. The triple compound deodorant is composed of Thiobacillus thifera, Rhodospirillum globosa, and plant lactic acid bacteria. The specific steps of preparation are as follows:

[0054] Step 1. Thiobacillus sphaericus was isolated from the black and odorous section of the Chuhe River in Liuhe District, Nanjing, and Rhodospirillum globosa and Lactobacillus plantarum were isolated from the bottom mud of Nanjing City Landscape River by our laboratory. Store in LB solid plates and store in a refrigerator at 4°C;

[0055] Step 2. Use an inoculation loop to pick up the three bacterial strains in step 1 and inoculate them into the liquid culture medium respectively, cultivate them on a shaking table at 30°C and 120rpm for 20 hours, and store them at 4°C until use.

[0056] Step 3, primary fermentation of bacterial strains: the three kinds of bacteria for use in step 2 are successively carried out primary fermentation and cultiva...

Embodiment 3

[0062] The invention also provides an application method of the triple compound deodorant, which is used to investigate the deodorizing effect of the triple compound deodorant through experiments.

[0063] Carry out the field test in the garbage disposal site, the temperature is 20-30 ℃, set up 5 sampling points in the center of the experimental area and its surroundings, spray 150ml of bacterial agent per square meter, and detect ammonia and hydrogen sulfide at 6H, 12H and 48H after spraying content and degradation rate (taking the average value), and set up a blank control group at the same time to investigate the deodorizing effect.

[0064] Determination of the degradation effect of the triple compound deodorant:

[0065]

[0066] In the early stage of the experiment, the ammonia degradation rate in the experimental group was relatively slow, and it degraded rapidly after two days, and the degradation rate reached 79% after four days. Among them, the degradation effect...

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Abstract

The invention relates to the technical field of biological environments, in particular to a preparation method of a triple composite deodorant. The triple composite deodorant is composed of thiobacillus thioparus, rhodospirillum sphaeroides and lactobacillus plantarum. The preparation method comprises the following specific steps of performing separation to obtain the thiobacillus thioparus, the rhodospirillum sphaeroides and the lactobacillus plantarum, and storing the strains in an LB solid flat plate; picking the three strains by using an inoculating loop, and inoculating the three strainsinto a liquid culture medium; and sequentially carrying out primary fermentation culture on the three strains, and carrying out composite inoculation on the three strains in a fermentation tank filledwith the liquid culture medium according to a ratio of 4: 3: 3 for secondary fermentation culture, thereby obtaining the liquid triple composite deodorant. The invention also provides an applicationmethod of the triple composite deodorant. According to the invention, the thiobacillus thioparus, the lactobacillus and photosynthetic bacteria are organically combined, so that the generation of odorous gases in black and odorous rivers and landfill leachate can be effectively reduced, and the self-repairing capability of the culture water environment is maintained.

Description

technical field [0001] The invention relates to the technical field of biological environment, in particular to the preparation and application method of a triple compound deodorant. Background technique [0002] Nowadays, due to the impact of human activities, the ammonia nitrogen and sulfide indicators in urban rivers and relatively open waters remain high, and the black and odor problem has seriously affected the urban environment. The ecological environment problems caused by this have already affected the human home environment and the image of the city. The main source of black and odorous gas is the volatilization of substances such as ammonia, hydrogen sulfide, methane, sulfide, and organic acids. These gases not only bring a lot of inconvenience to the lives of residents, but also have an important impact on human health. [0003] The existing deodorization technology mainly adopts chemical method, physical method and biological method. The chemical method has th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C02F101/16C02F101/10C12R1/01C12R1/25
CPCC02F3/34C02F2101/101C02F2101/16C02F2103/007C12N1/20
Inventor 王凯朱辉曹惠忠
Owner 南京师盛生态环境研究院有限公司
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