Immunomagnetic bead separation method capable of obtaining complete exosomes

Abstract

The invention relates to an immunomagnetic bead separation method capable of obtaining complete exosomes, which comprises the following steps: preparing a separation solution by using trypsin with a specific concentration as a main body, digesting antibody protein playing a role in connecting immunomagnetic beads and exosomes, and separating the immunomagnetic beads from the exosomes, thereby separating the exosomes from the immunomagnetic beads. Meanwhile, the integrity and the biological activity of the exosomes are kept, contained contents such as protein and nucleic acid are not damaged, and the exosomes can be further used for researching the form, the biological activity and the like of the exosome and characterizing and detecting TEM, NTA and the like.

Description

technical field [0001] The invention relates to the technical field of biological experiments, in particular to an immunomagnetic bead separation method capable of obtaining complete exosomes. Background technique [0002] Exosome: It is a kind of discoid vesicle with a diameter of 30-150nm secreted by most cells, which contains a variety of nucleic acids and proteins, and can exchange information between cells. When exosomes are formed by cell exocytosis, various proteins, lipids, RNA and some fusion genes in the cell will selectively enter these vesicles, so the exosomes carry many proteins derived from the mother cell. biological information. On the other hand, exosomes have the same membrane structure as cells, that is, phospholipid bilayers, which can well protect the proteins and nucleic acids they carry from external influences and maintain their original properties. [0003] At present, more and more studies have shown that the substances in exosomes are closely re...

AI Technical Summary

Problems solved by technology

At present, the existing exosome-magnetic bead separation method still has room for improveme

Images