High-phosphorus-loading living microalgae as well as preparation method and application thereof

A technology of microalgae and living body, applied in the field of environmental engineering, can solve the problems of high heavy metal treatment cost and unsatisfactory treatment effect, and achieve the effects of promoting the growth of microalgae, easy industrialization and application, and simplifying adsorption steps.

Pending Publication Date: 2020-09-04
WUHAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of this, the present invention aims to propose a method for preparing living microalgae with high phosphorus loading, so as to solve the problems of high heavy metal treatment cost and unsatisfactory treatment effect in the existing heavy metal-containing wastewater

Method used

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  • High-phosphorus-loading living microalgae as well as preparation method and application thereof
  • High-phosphorus-loading living microalgae as well as preparation method and application thereof
  • High-phosphorus-loading living microalgae as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] In this example, mediums with different phosphorus concentrations were used to modify microalgae with phosphorus to obtain different series of living microalgae with high phosphorus loading, which were specifically obtained by the following methods:

[0033] 1) BG-11 medium preparation: 1.5gNaNO 3 , K 2 HPO 4 (Change the amount added according to different modified media), 36mgCaCl 2 2H 2 O, 6 mg ammonium citrate-water, 6 mg ferric ammonium citrate, 1 mg EDTA, 2.86 μg H 3 BO 3 , 1.81 μg MnCl 2 4H 2 O, 0.222 μg ZnSO 4 ·7H 2 O, 0.39 μg NaMoO 4 ·5H 2 O, 0.079 μg CuSO 4 5H 2 O, 0.050 μg CoCl 2 ·6H 2 O, 1L sterile distilled water;

[0034] 2) Activation and modification of microalgae: inoculate the above-mentioned DidymogenespalatinaXR microalgae in mediums with phosphorus concentrations (calculated as P) of 40, 80, 160, and 200 mg / L according to the concentration of 0.08-0.1 g / L, at 25 ℃, 3600LX light intensity, and light-to-dark ratio of 14h:10h for cultiva...

Embodiment 2

[0038]The modified live algae prepared in Example 1 of the present invention, that is, high phosphorus-loaded living microalgae, is used to treat heavy metal-containing wastewater, wherein the heavy metal wastewater is specifically Cd(II)-containing wastewater, which specifically includes the following steps:

[0039] 1) calculate each modified living algae, i.e. the bioconcentration of four kinds of living algae (B-40, B-80, B-160, B-200) in embodiment 1, then, get respectively in embodiment 1 Four kinds of live algae (B-40, B-80, B-160, B-200) 5mg in a centrifuge tube (according to the liquid volume);

[0040] 2) Take 8 mL of Cd(II) solution with an initial concentration of 2 mg / L and add it to a centrifuge tube containing 5 mg of the above-mentioned algae sample, adjust the pH of the solution to 3.0, 4.0, 5.0, 6.0 at a temperature of 25 °C and a rotation speed of 150 rad / min Shake and adsorb on a vibrating shaker for 1.5 hours, and then centrifuge the algae liquid after the...

Embodiment 3

[0044] The modified live algae prepared in Example 1 of the present invention, that is, high phosphorus-loaded living microalgae, is used to treat heavy metal-containing wastewater, wherein the heavy metal wastewater is specifically Cd(II)-containing wastewater, which specifically includes the following steps:

[0045] 1) Take 5 mg of four kinds of live algae (B-40, B-80, B-160, B-200) in Example 1 respectively in a centrifuge tube (according to the liquid volume);

[0046] 2) Add 8 mL of Cd(II) solution with a concentration gradient of 2 to 30 mg / L to the centrifuge tube containing the above-mentioned four kinds of live algae, adjust the pH of the solution to 6.0, shake at a temperature of 25 °C and a speed of 150 rad / min The bed was shaken and adsorbed for 1.5 hours, and then the algae liquid after the adsorption was completed was centrifuged to obtain Cd(II)-containing biomass and Cd(II)-depleted wastewater (supernatant).

[0047] In this embodiment, the concentration of Cd...

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Abstract

The invention provides high-phosphorus-loading living microalgae as well as a preparation method and an application thereof. The preparation method comprises the following steps that microalgae is subjected to activation culture in a phosphorus-containing culture medium, and the high-phosphorus-loading living microalgae is obtained. According to the method, a phosphorus element is used for modifying the active microalgae to obtain the high-phosphorus-loading living microalgae, the adsorption capacity of the active microalgae to heavy metals can be greatly improved, and when the high-phosphorus-loading living microalgae is used for treating the heavy metals in the wastewater, the heavy metals in the wastewater can be effectively removed only through simple oscillation adsorption; accordingto the high-phosphorus-loading living microalgae disclosed by the invention, the adsorption capacity on cadmium in wastewater can reach 7.41 mg/g, the pollution of phosphorus to a water body can be reduced while the growth of the microalgae is promoted, and the total phosphorus concentration in sewage can be reduced to 0.2 mg/L or below, so that the high-phosphorus-loading living microalgae can reach the III-class standard of surface water environment quality to be discharged; and eutrophication of the water body caused by excessive phosphorus elements in the water body is avoided, so that thepurposes of removing heavy metals and phosphorus by the microalgae and treating sewage are achieved.

Description

technical field [0001] The invention relates to the technical field of environmental engineering, in particular to a high-phosphorus-loaded living microalgae and its preparation method and application. Background technique [0002] Phosphorus is the root cause of eutrophication in water bodies. At the same time, phosphorus is also a necessary nutrient for the growth of active microalgae. At present, there are mainly three methods for the disposal of phosphorus-containing wastewater: biochemical method, physical adsorption method and chemical phosphorus removal method. These methods are common. High cost and unsatisfactory phosphorus removal effect. [0003] Cadmium-containing wastewater is highly toxic, and cadmium compounds are more toxic. Cadmium ions are easy to accumulate in organisms. If cadmium-containing wastewater is not strictly treated, it will easily cause chronic poisoning of organisms, which is more harmful. Among them, cadmium chloride is the most toxic. When...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12N1/20C02F3/34C02F3/32C12R1/89C12R1/01C02F101/20C02F101/10
CPCC12N1/12C12N1/20C02F3/322C02F3/325C02F3/34C02F2101/20C02F2101/105
Inventor 夏令宋少先王芷芯黄容
Owner WUHAN UNIV OF TECH
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