High-phosphorus-loading living microalgae as well as preparation method and application thereof
A technology of microalgae and living body, applied in the field of environmental engineering, can solve the problems of high heavy metal treatment cost and unsatisfactory treatment effect, and achieve the effects of promoting the growth of microalgae, easy industrialization and application, and simplifying adsorption steps.
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Embodiment 1
[0032] In this example, mediums with different phosphorus concentrations were used to modify microalgae with phosphorus to obtain different series of living microalgae with high phosphorus loading, which were specifically obtained by the following methods:
[0033] 1) BG-11 medium preparation: 1.5gNaNO 3 , K 2 HPO 4 (Change the amount added according to different modified media), 36mgCaCl 2 2H 2 O, 6 mg ammonium citrate-water, 6 mg ferric ammonium citrate, 1 mg EDTA, 2.86 μg H 3 BO 3 , 1.81 μg MnCl 2 4H 2 O, 0.222 μg ZnSO 4 ·7H 2 O, 0.39 μg NaMoO 4 ·5H 2 O, 0.079 μg CuSO 4 5H 2 O, 0.050 μg CoCl 2 ·6H 2 O, 1L sterile distilled water;
[0034] 2) Activation and modification of microalgae: inoculate the above-mentioned DidymogenespalatinaXR microalgae in mediums with phosphorus concentrations (calculated as P) of 40, 80, 160, and 200 mg / L according to the concentration of 0.08-0.1 g / L, at 25 ℃, 3600LX light intensity, and light-to-dark ratio of 14h:10h for cultiva...
Embodiment 2
[0038]The modified live algae prepared in Example 1 of the present invention, that is, high phosphorus-loaded living microalgae, is used to treat heavy metal-containing wastewater, wherein the heavy metal wastewater is specifically Cd(II)-containing wastewater, which specifically includes the following steps:
[0039] 1) calculate each modified living algae, i.e. the bioconcentration of four kinds of living algae (B-40, B-80, B-160, B-200) in embodiment 1, then, get respectively in embodiment 1 Four kinds of live algae (B-40, B-80, B-160, B-200) 5mg in a centrifuge tube (according to the liquid volume);
[0040] 2) Take 8 mL of Cd(II) solution with an initial concentration of 2 mg / L and add it to a centrifuge tube containing 5 mg of the above-mentioned algae sample, adjust the pH of the solution to 3.0, 4.0, 5.0, 6.0 at a temperature of 25 °C and a rotation speed of 150 rad / min Shake and adsorb on a vibrating shaker for 1.5 hours, and then centrifuge the algae liquid after the...
Embodiment 3
[0044] The modified live algae prepared in Example 1 of the present invention, that is, high phosphorus-loaded living microalgae, is used to treat heavy metal-containing wastewater, wherein the heavy metal wastewater is specifically Cd(II)-containing wastewater, which specifically includes the following steps:
[0045] 1) Take 5 mg of four kinds of live algae (B-40, B-80, B-160, B-200) in Example 1 respectively in a centrifuge tube (according to the liquid volume);
[0046] 2) Add 8 mL of Cd(II) solution with a concentration gradient of 2 to 30 mg / L to the centrifuge tube containing the above-mentioned four kinds of live algae, adjust the pH of the solution to 6.0, shake at a temperature of 25 °C and a speed of 150 rad / min The bed was shaken and adsorbed for 1.5 hours, and then the algae liquid after the adsorption was completed was centrifuged to obtain Cd(II)-containing biomass and Cd(II)-depleted wastewater (supernatant).
[0047] In this embodiment, the concentration of Cd...
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