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Kit for early screening diagnosis

A kit and technology for use, applied in the field of kits for early screening and diagnosis, can solve problems such as joint bone damage, and achieve the effects of significant technological progress, convenient test results, and accurate test results.

Pending Publication Date: 2020-09-08
上海怡珏生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, patients with positive CCP antibodies are more prone to joint bone damage

Method used

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  • Kit for early screening diagnosis
  • Kit for early screening diagnosis
  • Kit for early screening diagnosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1 production process

[0024] 1.1 Preparation process of colloidal gold conjugates

[0025] 1.1.1 Preparation of colloidal gold

[0026] 1.1.1.1 Heat 300 ml of 0.05% gold chloride solution to boiling.

[0027] 1.1.1.2 Add 3.6 ml of 5% trisodium citrate.

[0028] 1.1.1.3 Continue heating and boiling for 15 minutes, stop heating, wait until it cools to room temperature, and restore to the original volume with distilled water.

[0029] 1.1.2 Colloidal gold labeling

[0030] 1.1.2.1 Colloidal gold 100ml.

[0031] 1.1.2.2 Add 1.2 ml of 1.0% anhydrous sodium carbonate solution, and adjust the pH value to 6.4-7.2.

[0032] 1.1.2.3 Add 2.5 ml of SPA (1 mg / ml) and let stand at room temperature for 30 minutes.

[0033] 1.1.2.4 Stir and add 20 ml of 10×Tris-HCl to adjust the pH to 7.2-7.5.

[0034] 1.1.2.5 Stir and add 2 g of BSA, 1 g of PEG20000, and 0.2 ml of ProClin300.

[0035] 1.2 Detection board preparation process

[0036] 1.2.1 Assemble the test board, ...

Embodiment 1

[0050] Implementation Example 1: Gold Label Assay Kit for Cyclic Citrullinated Peptide

[0051] Testing method

[0052] The gold calibration method was determined by the FT-J5 gold standard reader produced by Shanghai Beijia Biochemical Reagent Co., Ltd.

[0053] 1. Take out the reagents and samples before use, and place them at room temperature for 15-30 minutes to allow them to return to room temperature.

[0054] 2. Take out the test board.

[0055] 3. Add 2 drops of blocking solution to the center of the reaction well of the detection plate, and wait for the blocking solution to penetrate completely.

[0056] 4. Use a pipette to add 40 microliters of the sample to be tested in the center of the reaction well of the test plate, and wait until the sample is completely infiltrated.

[0057] 5. Add 4 drops of washing solution to the center of the test plate reaction well, and wait for the washing solution to penetrate completely.

[0058] 6. Add 3 drops of colloidal gold c...

Embodiment 2

[0078] Implementation example 2: immune latex enhanced turbidimetry kit

[0079] Instrument: 7100 biochemical analyzer

[0080] Operation method: The sample is measured by Hitachi 7100 biochemical analyzer. In the step of measuring the sample with a biochemical analyzer, add reagent IR1 to the sample, incubate at 37°C for 5 minutes, read the first point of sample A1, add reagent IIR2, incubate at 37°C for 5 minutes, and read the second point Sample A2, sample A=sample A2-sample A1; also includes a step of measuring the calibrator using a Hitachi 7100 biochemical analyzer, and adding the calibrator to the reagent in the step of using a biochemical analyzer to measure the calibrator After IR1, incubate at 37°C for 5 minutes, read the first point calibration A1, add reagent IIR2, incubate at 37°C for 5 minutes, read the second point calibration A2, calibration A=calibration A2-calibration A1;

[0081]

[0082] The parameters measured above are: temperature 37°C, wavelength 6...

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Abstract

The invention belongs to the field of bioengineering and provides new application of cyclic citrullinated peptide (CCP) antigen in preparing markers for detecting peripheral serum, whole blood and peripheral blood for diagnosing cardiac or cerebral thrombosis, cardiac or cerebral infarction, cerebral hemorrhage, cardiac or cerebral embolism and tumor markers, thyroid cancer, liver cancer, stomachcancer, uterine cancer, cervical cancer, lung cancer, breast cancer, esophageal cancer, nasopharynx cancer, prostate cancer, colorectal cancer, kidney cancer, pancreatic cancer, bladder cancer, bacterial infection or virus infection. According to the kit disclosed in the invention, detection is carried out through a CCP antigen-antibody binding reaction principle; the kit can be applied to diagnosis of cardiac or cerebral thrombosis, myocardial or cerebral infarction, cerebral hemorrhage, cardiac or cerebral embolism, bacterial infection or virus infection and tumor markers by adopting an immune latex enhancement method, a Pock instant test method, an ELISA method, a chemiluminiscence method, a microfluidic chip method, a colloidal gold infiltration method and a chromatography method. Byadopting the kit, a detection report can be received on the same day, and the detection result is accurate and convenient.

Description

technical field [0001] The present invention relates to the field of bioengineering, especially kits, especially kits using cyclic citrullinated peptide (CCP) antigens for detecting peripheral serum, whole blood and peripheral blood to diagnose cardiac or cerebral thrombosis, cardiac or cerebral infarction, cerebral hemorrhage, Cardiac or cerebral embolism, bacterial infection, viral infection, tumor markers: thyroid cancer, liver cancer, gastric cancer, uterine cancer, cervical cancer, lung cancer, breast cancer, esophagus cancer, nasopharyngeal cancer, prostate cancer, colorectal cancer, kidney cancer , pancreatic cancer, bladder cancer. Background technique [0002] Cyclic citrullinated peptide (CCP) replaces two serines in a linear citrullinated peptide containing 19 amino acids with cysteine ​​to form a disulfide bond similar to the β-turn structure and become a cyclic citrullinated peptide. [0003] Citrulline is a major constitutive epitope component recognized by a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/574G01N33/569
CPCG01N33/6893G01N33/57488G01N33/57407G01N33/56911G01N33/56983G01N2333/47G01N2800/324
Inventor 朱梅珍刘颖冰
Owner 上海怡珏生物科技有限公司
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