Live clostridium butyricum preparation and production method thereof, animal feed additive

Abstract

The invention provides a live Clostridium butyricum preparation and a production method thereof, and an animal feed additive. The production method of a Clostridium butyricum live preparation comprises the following steps: a culture medium preparation step, a seed liquid preparation step and a fermentation step, and a culture medium preparation The solid-state fermentation medium in the step of preparing the solid-state fermentation medium includes bee pollen; the step of preparing the seed solution includes inserting the activated Clostridium butyricum species into the seed medium obtained in the step of preparing the seed medium to obtain Seed liquid; the fermentation step includes inserting the seed liquid obtained in the above steps into the solid-state fermentation medium obtained in the solid-state fermentation medium preparation step, and after the fermentation is completed, crushing the solid-state medium to obtain a live Clostridium butyricum preparation, Clostridium butyricum Under the combined effect of solid-state fermentation and the antioxidant properties of bee pollen, the conversion rate of spores is high, and the growth performance of animals can be improved after animals eat Clostridium butyricum fermented by solid-state fermentation of bee pollen.

Description

technical field [0001] The invention relates to the field of Clostridium butyricum fermentation technology, in particular to a live Clostridium butyricum preparation, a production method thereof, and an animal feed additive. Background technique [0002] Clostridium butyricum is an obligate anaerobic Gram-positive bacillus that can colonize the intestinal tract of animals and produce probiotics mainly short-chain fatty acids such as butyric acid. Since the EU approved Clostridium butyricum in 2003 Since bacteria were used as feed additives for broilers and weaned piglets, they have been gradually promoted as probiotic feed additives. A large number of research results show that Clostridium butyricum can produce rich amylase, group B vitamins, etc., and promote digestion and absorption; at the same time, Clostridium butyricum can inhibit the growth of harmful bacteria and promote the proliferation of beneficial bacteria, regulating intestinal flora imbalance; butyric acid Bu...

AI Technical Summary

Problems solved by technology

[0004] However, the reducing ability of the substrate in the fermentation medium comprising the above-mentioned fermentation substrate is relatively poor, so that the activity of Clostridium butyricum fermented in the fermentation medium is relatively poor, and the livestock and poultry that eat this kind of Clostridium butyricum end up Growth performance does not meet the needs of the current market

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