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High-efficiency breeding method of ultra-high-strength brewer's yeast strains

A technology of beer yeast and strains, applied to beer fermentation methods, beer brewing, and microbial-based methods, etc., can solve the problems of cumbersome steps, long fermentation cycle, and low screening efficiency, and achieve high efficiency in the screening process and simple steps in the screening process Effect

Active Publication Date: 2022-07-19
TSINGTAO BREWERY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Aiming at the problems of long fermentation period, cumbersome steps and low screening efficiency in the prior art, the present invention proposes a high-efficiency breeding method for ultra-high-concentration beer yeast strains with simple operation, high screening efficiency and good performance

Method used

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  • High-efficiency breeding method of ultra-high-strength brewer's yeast strains
  • High-efficiency breeding method of ultra-high-strength brewer's yeast strains
  • High-efficiency breeding method of ultra-high-strength brewer's yeast strains

Examples

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Embodiment 1

[0075] The present embodiment provides a high-efficiency breeding method for ultra-high-strength brewer's yeast strains, and the specific method is:

[0076] ·Medium formula:

[0077] YPD solid medium (with ethanol and sorbitol added): yeast extract 1%, peptone 2%, maltose 1%, absolute ethanol 10%, sorbitol 30%, agar 2%;

[0078] YPD liquid medium: yeast extract 1%, peptone 2%, maltose 1%;

[0079] Primary screening medium: yeast extract 1%, peptone 2%, maltose 1%, absolute ethanol 10%, sorbitol 30%, 2-deoxy-D-glucose 0.05%, agar 2%;

[0080] Wort medium: the concentration of extra-high-concentration wort is 32°P, and the addition of absolute ethanol is gradually increased from 6% to 16%.

[0081] Experimental equipment: constant temperature incubator, automatic high-throughput microbial droplet incubator, etc.

[0082] ·experimental method:

[0083] (1) the alternative bacterial strain is coated on the YPD solid medium plate that adds ethanol and sorbitol, according to th...

Embodiment 2

[0090] The present embodiment utilizes the selection and breeding method provided in Example 1 to screen to obtain 10 double-screened strains for EBC fermentation experiments. The specific methods are as follows:

[0091] Using the screening method provided in Example 1 of the present invention, 10 strains of re-screened strains were obtained, and the strain numbers were 8#, 10#, 11#, 21#, 38#, 39#, 46#, 55#, 61#, 4 -3#, carry out the EBC tube fermentation test of the above-mentioned yeast strain in 32°P extra-high-concentration wort, and screen out the yeast strain suitable for extra-high-concentration brewing by evaluating the fermentation index and maturity index of the above-mentioned yeast strain. Among them, the experimental operation and experimental results of EBC tube fermentation are as follows:

[0092] (1) Experimental materials and methods:

[0093] ·Experimental grouping:

[0094] Experimental group: 8#, 10#, 11#, 21#, 38#, 39#, 46#, 55#, 61#, 4-3#;

[0095] Con...

Embodiment 3

[0110] The present embodiment utilizes the relevant results obtained in Example 2 to carry out genetic stability experiments on the rescreened strains, and the specific methods are as follows:

[0111] ·experimental method:

[0112] According to a series of results obtained in Example 2, according to the fermentation degree, beer maturity (diacetyl and acetaldehyde content in beer) and other indicators, the yeast strains No. 21# and 61# were selected for passage and domestication to verify their genetic stability. The specific method is as follows: pass the 21# and 61# yeast strains in 32°P extra-high-concentration wort, and after 10 generations, carry out the EBC tube fermentation test with the passaging yeast and the original yeast (the experimental method is the same as that in Example 2) , to compare the fermentation performance indexes of the two yeast strains before and after passage.

[0113] ·Experimental results:

[0114] The comparison chart of fermentation degree,...

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Abstract

The invention provides a high-efficiency breeding method for ultra-high-strength brewer's yeast strains, which belongs to the field of strain breeding. The breeding method includes the steps of screening starting strains, screening primary screening strains, culturing the adaptability of the primary screening strains, and evaluating strains. Using the high-efficiency breeding method provided by the present invention, an extra-high-strength brewer's yeast strain TG-01 is obtained by screening, and its preservation number is CGMCC No. 19839, which was preserved in the Common Microorganisms of the China Committee for the Preservation and Administration of Microorganisms on May 26, 2020 center. The present invention combines 2-deoxy-D-glucose, sorbitol and alcohol to simulate an ultra-high-concentration fermentation environment higher than large-scale production by establishing a method of combining high-efficiency screening and adaptive evolution screening under specific stress conditions It can further screen out target strains that are insensitive to glucose, hypertonic, and alcohol-tolerant. It has a very broad application prospect in the field of strain selection.

Description

technical field [0001] The invention belongs to the field of strain selection and breeding, and in particular relates to a high-efficiency selection and breeding method for ultra-high-strength brewer's yeast strains. Background technique [0002] Ultra-high-concentration brewing technology is a new trend in the development of beer industry at home and abroad, which can greatly reduce energy consumption and improve equipment utilization. The higher the wort concentration, the more prominent the advantages. Compared with high-concentration brewing, extra-high-concentration brewing (≥22°P) puts forward higher technical requirements and difficulties for yeast strains, such as extra-high-concentration wort, high osmotic pressure and other environments will affect the normal physiological and biochemical characteristics of yeast , reduce yeast activity and fermentation performance, and ultimately affect the flavor and coordination of beer. Therefore, the premise of realizing ultr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/36C12N1/18C12C11/02C12R1/85
CPCC12N1/36C12N1/18C12C11/02C12R2001/85C12N1/185
Inventor 尹花董建军余俊红陈璐万秀娟赵玉祥陈嵘侯晓平贺扬
Owner TSINGTAO BREWERY