Sodium alginate double-layer drug-loaded microspheres and preparation method thereof

A technology of sodium alginate and drug-loaded microspheres is used in pharmaceutical formulations, devices for making drugs into special physical or taking forms, drug combinations, etc. Problems such as poor fusion of regenerated cartilage, to achieve the effect of large market application potential, helpful for popularization and use, and simple preparation process

CN111671728BActive Publication Date: 2021-11-30NANJING CHILDRENS HOSPITAL
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Publication Date
2021-11-30

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Abstract

The invention discloses a sodium alginate double-layer drug-loaded microsphere and a preparation method thereof. The outer layer of the microsphere is loaded with ENBREL+Bevacizumab, and the inner layer is loaded with IGF-1, which is mainly through a flow focusing method and a microfluidic control method. Prepare. Applied in the process of preventing osteogenic differentiation and bone bridge formation, it can effectively control the release of anti-osteobridge formation factors (Enbrel+Bevacizumab) and chondrogenic differentiation factors (IGF‑1) in the local sequence of epiphyseal plate damage, and the outer layer released early Anti-bone bridge formation factor (Enbrel+Bevacizumab) can prevent osteogenic differentiation and bone bridge formation, and the subsequently released inner layer IGF-1 can promote chondrogenic differentiation and epiphyseal cartilage regeneration, which is of great significance to the repair of epiphyseal plate injury.
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Description

technical field

[0001] The invention relates to a drug-loaded microsphere, in particular to a sodium alginate double-layer drug-loaded microsphere and a preparation method thereof. Background technique

[0002] Physeal plates, also known as growth plates, are areas of cartilage at the ends of immature long bones in children that control bone longitudinal growth. Fractures, infections, tumors, or iatrogenic injuries can all lead to damage to the epiphyseal plate. About 30% of children with fractures are accompanied by damage to the epiphyseal plate and bone growth imbalance. Once the epiphyseal plate is damaged, the cartilage tissue of the epiphyseal plate will be gradually replaced by bone tissue, forming a "bone bridge", leading to growth retardation, angulation or rotation deformity, and even further affecting joint function, which will have a huge impact on children's physical and mental health. The current clinical treatment method is mainly bone bridge resection combin...

Examples

Embodiment 1

[0026] Example 1: Preparation of a kind of sodium alginate double-layer drug-loaded microspheres

[0027] In this embodiment, the preparation of sodium alginate double-layer drug-loaded microspheres is mainly carried out by using the flow focusing method and the microfluidic control method, and the main steps are as follows:

[0028] 1. If figure 2 As shown, the microfluidic chip is placed on the stage of the optical microscope, and six flexible tubes are respectively connected to extend out the six channel ports;

[0029] 2. Add the 1% sodium alginate solution with IGF-1 into the single-channel syringe pump through the syringe, exhaust the air, and connect it to the No. 2 channel port through the hose;

[0030] 3. Draw 1% sodium alginate solution into two syringes, place them in a dual-channel syringe pump, and connect them to the two No. 1 channel ports through a hose;

[0031] 4. Use two syringes to draw 1% CaCl 2 The paraffin oil is placed in a dual-channel syringe pum...