Sodium alginate double-layer drug-loaded microspheres and preparation method thereof

Abstract

The invention discloses a sodium alginate double-layer drug-loaded microsphere and a preparation method thereof. The outer layer of the microsphere is loaded with ENBREL+Bevacizumab, and the inner layer is loaded with IGF-1, which is mainly through a flow focusing method and a microfluidic control method. Prepare. Applied in the process of preventing osteogenic differentiation and bone bridge formation, it can effectively control the release of anti-osteobridge formation factors (Enbrel+Bevacizumab) and chondrogenic differentiation factors (IGF‑1) in the local sequence of epiphyseal plate damage, and the outer layer released early Anti-bone bridge formation factor (Enbrel+Bevacizumab) can prevent osteogenic differentiation and bone bridge formation, and the subsequently released inner layer IGF-1 can promote chondrogenic differentiation and epiphyseal cartilage regeneration, which is of great significance to the repair of epiphyseal plate injury.

Description

technical field [0001] The invention relates to a drug-loaded microsphere, in particular to a sodium alginate double-layer drug-loaded microsphere and a preparation method thereof. Background technique [0002] Physeal plates, also known as growth plates, are areas of cartilage at the ends of immature long bones in children that control bone longitudinal growth. Fractures, infections, tumors, or iatrogenic injuries can all lead to damage to the epiphyseal plate. About 30% of children with fractures are accompanied by damage to the epiphyseal plate and bone growth imbalance. Once the epiphyseal plate is damaged, the cartilage tissue of the epiphyseal plate will be gradually replaced by bone tissue, forming a "bone bridge", leading to growth retardation, angulation or rotation deformity, and even further affecting joint function, which will have a huge impact on children's physical and mental health. The current clinical treatment method is mainly bone bridge resection combin...

AI Technical Summary

Problems solved by technology

Although a lot of research has been done before, using different seed cells, screening different types of bioactive materials and regulation methods, but still facing the problems of low scaffolding cartilage formation efficiency and poor fusion of regenerated cartilage and surrounding normal tissues

More importantly, the regenerated cartilage does not have a layered structure similar to the natural epiphyseal plate, and cannot control the longitudinal growth of long bones

The key reasons for this situation are mainly the following two points: 1. Scaffold micro-composition: At present, the research on the micro-composition of scaffolds mainly focuses on how to promote chondrogenic differentiation and regeneration of cartilage tissue, ignoring the prevention of local osteogenic differentiation and bone bridge formation after epiphyseal plate injury from the source in the early stage of injury;2 . Scaffold microstructure: At present, the microstructure of tissue engineering scaffolds does not conform to the physiological structure characteristics of the epiphyseal plate, resulting in the disordered accumulation of regenerated chondrocytes, which cannot exert its biological function of controlling the longitudinal growth of osteogenesis

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