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Molecular marker of cotton fiber elongation rate major QTL in range of qFE-chr.D04 and application

A technology of molecular markers and elongation rate, applied in the field of molecular biology, can solve the problems of long cycle, slow breeding progress, low efficiency, etc.

Pending Publication Date: 2020-09-22
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a molecular marker linked with the main effect QTL of upland cotton fiber elongation, so as to solve the problem of slow improvement of fiber elongation by conventional breeding
[0006] In order to overcome many shortcomings such as poor accuracy of phenotypic selection, low efficiency, long cycle and high cost in traditional breeding, and solve the problem of slow progress in breeding quality traits such as fiber elongation

Method used

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  • Molecular marker of cotton fiber elongation rate major QTL in range of qFE-chr.D04 and application
  • Molecular marker of cotton fiber elongation rate major QTL in range of qFE-chr.D04 and application

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Experimental program
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Effect test

Embodiment 1

[0022] 1. Field trials of 1,245 cotton core germplasm resources were carried out in Anyang, Henan, Shijiazhuang, Hebei, Dafeng, Jiangsu, Liuyang, Hunan, Kuche, Xinjiang, Aral, Shihezi Cotton Institute, Shihezi Agricultural Reclamation Academy of Sciences for two consecutive years in 2016 and 2017 respectively. A total of 8 test points were carried out. The experiment was set for 2 repetitions, and the normal field cultivation management. During the vegetative growth period of cotton, each individual plant was sampled and DNA extracted, and sent to Novozhiyuan Company for genome resequencing. During the peak period of cotton bolling, harvest 1-2 normal bolls on the first and second fruit nodes in the middle of each normal cotton plant, a total of 30. After ginning, use HVI1000 for fiber quality testing of lint samples, data collection Refer to the specification for the description of cotton germplasm resources.

[0023] 2. GWAS analysis: In the association panel of 1245 sampl...

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Abstract

The invention discloses a molecular marker of a cotton fiber elongation rate major QTL in a range of qFE-chr.D04 and application. The molecular marker is one or more of nucleotides shown from SEQ ID NO.1 to SEQ ID NO.66 as shown in the description, and causes polymorphism of a fiber elongation rate. The provided SNP molecular marker related to the upland cotton fiber elongation rate can be used for early prediction and screening of a cotton fiber elongation rate character, and can be used for breeding assisted by cotton fiber elongation rate molecular markers. According to a method, mainly bydirectly detecting DNA, the provided SNP molecular marker related to the upland cotton fiber elongation rate can be applied to various tissue of cotton in various development stages of the cotton, andare not limited by seasons or environments, and the problems of expression or non-expression and the like do not exist.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and relates to a molecular marker and application of a main QTL qFE-chr.D04 interval related to cotton fiber elongation. Background technique [0002] Cotton fiber is an excellent natural fiber and the leading raw material of the textile industry. It occupies an important position in the world and our national economy. Fiber quality is a complex quantitative trait controlled by multiple genes, and its constituent factors include: fiber length, strength, fineness, elongation, uniformity, maturity, etc. The phenotypic value of cotton fiber quality is not only controlled by the genetic factors of the variety, but also affected by a variety of environmental factors and the interaction between the variety and the environment. The elongation of upland cotton fiber is also one of the important indicators of fiber quality. The elongation at break of cotton fiber refers to the percentage of the...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 陈保军何守朴杜雄明潘兆娥贾银华李洪戈耿晓丽王立如庞保印
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI