Vertical-plate constant-current-type bioartificial liver reactor

Abstract

The invention discloses a vertical-plate constant-current-type bioartificial liver reactor. The vertical-plate constant-current-type bioartificial liver reactor comprises a reactor support, wherein the reactor support includes multiple layers of bottom plates which are arranged in parallel, a plurality of partitions are arranged between every two bottom plates, and are used for dividing channels between every two bottom plates, and a plurality of liquid flow channels are formed between every two bottom plates and multiple partitions; and gel layers are laid on the parts, in the liquid flow channels, of every two bottom plates, a cell layer is adhered to the surface of each gel layer, and a filter screen perpendicular to each bottom plate is installed at the upper part and lower part of thereactor support when the reactor support is arranged upright. According to the novel bioartificial liver reactor, the hollow channels are formed in the polypropylene vertical support through the bottom plates and the partitions so as to be adopted as the liquid flow channels, and liquid flow can be guided stably through the liquid flow channels, so that a liquid shear force is kept constant and suitable; and a sufficient source of hepatocytes is provided by stem cells, and a culture system which adopts interaction of hepatocytes, non-parenchymal cell clusters and matrix, so that the functionof the reactor is improved further.

Description

technical field [0001] The invention belongs to the field of bioreactors, in particular to a vertical plate constant flow bioartificial liver reactor. Background technique [0002] At present, the basic principle of the bioartificial liver is to culture liver cells or tissues in a special bioreactor, and use an extracorporeal circulation device to introduce blood or plasma from patients with liver failure into the bioreactor, through the semipermeable membrane in the reactor and the Liver cells carry out material exchange and biological action. The main factors affecting the function of biological artificial liver are the source of cells in the bioreactor and the selection of cell culture methods. Primary hepatocytes are difficult to expand in vitro and maintain their functions. Therefore, liver cancer cell lines or pig hepatocytes are often used as the main biological Material. The culture methods include plate, suspension or scaffold culture, which can be divided into sc...

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Problems solved by technology

[0006] The purpose of the present invention is to provide a constant-flow bioartificial liver reactor with vertical plates, which solves the problems of the difficulty in the expansion of primary hepatocytes and the loss of the liver microenvironment in vitro culture, which leads to the bottleneck in the preparation of the bioreactor.

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