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A method for identifying abnormal pathways of energy metabolism in depression based on stable isotope tracer metabolomics

A stable isotope and energy metabolism technology, applied in the field of identification of abnormal energy metabolism pathways in depression based on stable isotope tracer metabolomics, can solve the problem of being unable to distinguish which metabolic pathway the metabolites come from

Active Publication Date: 2022-03-18
SHANXI UNIV
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Problems solved by technology

The combination of stable isotope tracing technology and metabolomics technology can just make up for the defect that conventional metabolomics can only detect the sum of terminal metabolites, but cannot distinguish which metabolic pathway the metabolites come from

Method used

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  • A method for identifying abnormal pathways of energy metabolism in depression based on stable isotope tracer metabolomics
  • A method for identifying abnormal pathways of energy metabolism in depression based on stable isotope tracer metabolomics
  • A method for identifying abnormal pathways of energy metabolism in depression based on stable isotope tracer metabolomics

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Embodiment 1

[0030] Example 1: Based on the method of stable isotope tracer identification of abnormal energy metabolism pathways in depression, stable isotope labeling is carried out on energy metabolism precursor glucose, and comprehensive characterization of energy metabolism compounds is carried out by using normal phase chromatographic columns and reversed phase chromatographic columns. The isotope distribution characteristics of the downstream metabolites of glucose clarified the exact metabolic pathway of abnormal energy metabolism in depression.

[0031] details as follows:

[0032] (1) Labeled precursors of energy metabolism 13 C 6 -Glucose was introduced into the depression-like rats and blank control rats, samples were collected after continuous tail vein injection for 6 hours, and unlabeled samples were collected before the introduction of the isotope tracer for subsequent identification.

[0033](2) High-performance liquid chromatography-mass spectrometry (HPLC-MS) full-scan...

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Abstract

The present invention is the field of stabilizing homogeneous traceing and metabolic group. It provides a method based on stable homogenic traceing and identification of depression and energy metabolic abnormal pathway.The color spectrum column comprehensively represents the energy metabolic compound, and according to the distribution characteristics of the isotope distribution of the lower reaches of the glucose, it is clear that the exact metabolic pathway of the abnormal metabolic energy metabolism is clear.The method provided by the present invention has important scientific values for understanding depression and development of new antidepressant drugs, providing new methods for exploring the mechanism of the disease and the discovery of drug targets and the discovery of drug targets and the development of new drugs.

Description

technical field [0001] The invention belongs to the technical field of stable isotope tracer metabolomics, and specifically relates to a method for identifying abnormal pathways of energy metabolism in depression based on stable isotope tracer metabolomics. Based on the research on the abnormal energy metabolism pathway of depression with this technology, it will open up a new way to understand the pathogenesis of depression and discover potential targets of the disease. Background technique [0002] The serious harm of depression to human life has attracted great attention. However, due to the unknown and complex pathogenesis, the existing marketed antidepressant drugs developed based on the theory of monoamine neurotransmitters are not ideal, and the drug control rate is only about 30%. Although a variety of pathogenesis understandings have been developed, such as neuroendocrine, neuroimmune, neurotrophic, and inflammatory hypotheses, no ideal antidepressant drugs develope...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/72G01N30/86
CPCG01N30/02G01N30/06G01N30/72G01N30/8682
Inventor 令狐婷秦雪梅田俊生高耀张丽增
Owner SHANXI UNIV