Method for labeling escherichia coli proteome by using SILAC (Stable Isotope Labeling with Amino Acids in Cell Cultures) and special culture medium
A technology of Escherichia coli and culture medium, applied in the fields of peptide preparation methods, microorganism-based methods, chemical instruments and methods, etc., can solve the problems of hindering the progress of Escherichia coli quantitative proteomics, lack of labeling methods, etc., and achieve the elimination of genetic conditions. The effect of limiting, eliminating tedious steps, and efficient marking
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Embodiment 1
[0049] Embodiment 1, SILAC marker Escherichia coli special culture medium
[0050] Table 1 is the composition of the special medium for SILAC-labeled Escherichia coli
[0051]
[0052] Remarks: Inorganic reagents are ordinary analytical pure domestic reagents, light-labeled amino acids were purchased from Amresco Company in the United States, L-lysine- 13 C 6 Purchased from American CIL Company, article number: CNLM-291-0.25.
[0053] According to the above-mentioned components and their final concentrations, prepare with ultrapure water to obtain a special medium for SILAC-labeled Escherichia coli. Inorganic salts and glucose are sterilized by autoclaving, and amino acids and vitamins are sterilized by filtration.
Embodiment 2
[0054] Embodiment 2, the application of SILAC marker Escherichia coli special medium
[0055] 1. SILAC-labeled E. coli special medium to mark E. coli
[0056] 1. SILAC mark
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