Eye repair essence and preparation method thereof
An essence and eye technology, applied in skin care preparations, pharmaceutical formulas, cosmetic preparations, etc., can solve problems such as inability to effectively improve dark circles at the same time, to promote eye microcirculation, protect and strengthen eye capillaries Blood vessels, excellent effect of eliminating melanin
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Embodiment 1
[0033] Embodiment 1 Soybean composite type explant preparation
[0034] The preparation steps of the soybean composite explant include: taking soybean seeds, washing and sterilizing them, cultivating them in a germination medium to accelerate germination, and removing the seed coat from the eye of the seeds after the acceleration of germination.
[0035] The composition of the germination medium is: 20g / L glucose and 0.8% agar by mass percentage, pH5.6.
Embodiment 2
[0036] Embodiment 2, lactobacillus / soybean composite type explant fermentation product preparation
[0037] S1. Wash the soybean composite explant prepared in Example 1, dry it, add distilled water, make a slurry, filter to remove residue, and sterilize the slurry at 121° C. to obtain a culture solution;
[0038] S2. Mix Lactobacillus plantarum with sterilized milk, culture at 36°C for 24 hours, take 1ml from the cultured milk mixture and inoculate MRS broth medium, and cultivate for 48 hours;
[0039] S3. Inoculate the Lactobacillus after the secondary culture into the above-mentioned culture solution with 10% inoculation amount, add 1% glucose, and ferment for 3 days at 37°C. After that, it is obtained; during the fermentation process: the tank pressure is 0.01-0.03MPa; the ventilation volume is 1-3vvm; the rotation speed is 140rpm.
Embodiment 3
[0040] Embodiment 3, preparation of C. nigricans fermentation product extract
[0041] Step 1. Inoculate C. nigritiformis in a seed tank at an inoculum amount of 6%, and cultivate it for 4 days at a pH of 5.0 to 5.5 and a temperature of 25° C.; the composition of the seed medium is as follows: every 1000 ml of water contains: Glucose 30g, ammonium sulfate 2g, dipotassium hydrogen phosphate trihydrate 1g, glutamic acid 3g, triammonium citrate 3g and zinc sulfate 3mg;
[0042] Step 2: Transfer the C. nigricans seed culture solution from Step 1 to the fermentation medium containing the fermentation substrate according to 10v / v%, and cultivate it for 6 days at a pH of 5.3 to 5.6 and a culture temperature of 25°C. Fermentation is terminated after being filled with hyphae; the stirring speed is 100-200rpm; the ventilation volume is 1-4vvm; the tank pressure is 0.02-0.06MPa;
[0043]Step 3, take the fermentation filtrate, concentrate, let it stand for 12 hours, add 1.2 times the vol...
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