Non-invasive integrated fiber-based C-reactive protein sensor

Abstract

The invention provides a non-invasive integrated fiber-based C-reactive protein sensor. The protein sensor comprises a fiber-based body fluid filtering unit and a fiber-based organic electrochemical transistor detection unit, wherein the fiber-based body fluid filtering unit comprises a hydrophilic filtering area and a hydrophobic area surrounding the hydrophilic filtering area, the hydrophilic filtering area comprises a plurality of porous nanofiber membrane filtering layers of which the pore diameters are gradually reduced from top to bottom, and the surfaces of the porous nanofiber membranefiltering layers are modified with modifiers for filtering interfering substances in body fluid; a fiber-based organic electrochemical transistor is arranged below the hydrophilic filtering area andcomprises an electrolyte, a source-drain fiber electrode and a grid fiber electrode, and the surface of the grid fiber electrode is modified with anti-C-reactive protein monoclonal antibody targetingmolecules used for detecting C-reactive protein in body fluid. The transconductance value of the fiber-based C-reactive protein sensor provided by the invention is up to 40-100mS, and the fiber-basedC-reactive protein sensor has high sensitivity and low detection lower limit, can realize integration of body fluid filtration and sensing, and is suitable for wearing.

Description

technical field [0001] The invention belongs to the technical field of biological sensing and detection, and relates to a non-invasive integrated fiber-based C-reactive protein sensor. Background technique [0002] When the human body is stimulated by microorganisms (bacteria, viruses, etc.) invasion, tissue damage, or acute inflammation, liver cells will rapidly synthesize an acute phase protein, C-reactive protein (CRP). The sensitivity of CRP concentration changes is extremely high, even earlier than Changes in white blood cell count. CRP is an important biomarker for the auxiliary diagnosis of inflammatory response and early novel coronavirus infection pneumonia, and is commonly found in body fluids (such as blood, urine, saliva, etc.). At present, CRP is widely used in the clinical early diagnosis and differential diagnosis of cardiovascular diseases. In addition, the determination of CRP is also of positive significance for the treatment and prognosis of tumors. There...

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Problems solved by technology

For example, the invention patent CN106370861B discloses a C-reactive protein saliva detection test strip and its preparation method, including a nitrocellulose reaction membrane coated with a detection line of C-reactive protein monoclonal antibody and a quality control line of goat anti-mouse IgG. Polyester membrane gold label pad with colloidal gold-labeled C-reactive protein monoclonal antibody. The test strip uses saliva as the detection sample. Although the operation is simple and suitable for on-site detection, the minimum detection amount is only 10mg / L. And quantitative detection cannot be realized, and the sensitivity is not high enough

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