Novel coronavirus IgG and IgM antibody detection kit and preparation method and application thereof

Abstract

The invention provides a novel coronavirus IgG and IgM antibody detection kit and a preparation method and application thereof. A test strip in the kit comprises a bottom plate, and a sample pad, a colloidal gold pad, a nitrocellulose membrane and absorbent paper which are sequentially lapped and adhered on the bottom plate, the colloidal gold pad is coated with a colloidal gold labeled nucleocapsid protein, a colloidal gold labeled spinous process protein and a colloidal gold labeled rabbit IgG antibody compound; an IgG antibody detection line and an IgM antibody detection line are arranged on the nitrocellulose membrane. According to the invention, a capture method colloidal gold immunochromatography technology is adopted to realize rapid, sensitive and accurate detection of IgG antibodies and / or IgM antibodies.

Description

technical field [0001] The invention belongs to the technical field of biological immune detection and analysis, and relates to a novel coronavirus IgG and IgM antibody detection kit and a preparation method and application thereof. Background technique [0002] Coronaviruses are a class of single-stranded positive-sense RNA viruses with an envelope (envelope), with a diameter of about 60-220 nm, and widely exist between humans and other mammals. Most coronavirus infections are mild, but two coronaviruses have had outbreaks with severe consequences: severe acute respiratory syndrome coronavirus (SARS-CoV) and Middle East respiratory syndrome coronavirus (MERS-CoV). [0003] The emergence of a new type of coronavirus (2019-nCoV) at the end of 2019 has attracted widespread attention from all walks of life. The virus can be transmitted through respiratory droplets and contact. The basic reproduction number R0 is about 2.2 (90% high density interval 1.4-3.8). [0004] At presen...

AI Technical Summary

Problems solved by technology

Among them, nucleic acid detection has the advantages of high sensitivity, but it cannot realize the rapid detection of many suspected patients

PCR-based nucleic acid detection requires dedicated molecular biology laboratories and testing instruments, and requires experimenters to have a background in molecular biology. Grassroots units are limited by experimental conditions and experimenters, making it difficult to carry out new coronavirus detection based on nucleic acid detection

Nucleic acid detection takes a long time, an average of 4 to 5 hours, and the efficiency is low. Usually, repeated tests are required to confirm the results. Improper sample collection can easily lead to false negative or false positive results

At present, this method mainly collects samples through nasopharyngeal swabs and oropharyngeal swabs. However, the residence time of the new coronavirus in the upper respiratory tract (nose and mouth) is short, and it mainly gathers in the lower respiratory tract. The throat swab collection method is likely to cause false negative results. , causing the problem of low accuracy

At the same time, this method also has very strict requirements on sample transportation and storage, and improper operation may easily lead to false negative and false positive results.

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