Preparation method and application of ginsenoside composition

A technology of ginsenoside and composition, which is applied in the field of preparation of ginsenoside composition, can solve the problems of low yield of rare ginsenoside and the like, and achieve the effect of strong repairing ability

Pending Publication Date: 2020-10-13
JALA GROUP CORPORATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to provide a preparation method and application of a ginsenoside composition in order to overcome the defect of low yield of rare ginsenosides produced by Pichia pastoris in the prior art

Method used

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  • Preparation method and application of ginsenoside composition
  • Preparation method and application of ginsenoside composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1 Pichia pastoris transforms rare ginsenosides

[0039] 1. Preparation of 1% ginseng whole plant extract

[0040] According to the ratio (weight ratio) of roots: stems and leaves: flowers: seeds = 90:5:2.5:2.5, take 200g of ginseng whole plant samples, crush them to about 20 mesh with a pulverizer, add 2000mL of deionized water, and heat in a water bath at 96°C 2h, filter and centrifuge to obtain the filtrate; add 2000mL deionized water to the filter residue, heat in water bath for 2h, filter and centrifuge to obtain the filtrate; % Panax ginseng whole plant extract freeze-dried powder.

[0041] 2. Experiment of transformation of ginsenoside by Pichia pastoris

[0042] Using potato dextrose broth (1L medium containing 0.6% potato extract, 2.0% glucose, pH 5.6±0.2) as the seed medium, pick the bacterial liquid in the -80°C preservation tube of SP-02-DZ-08, Culture in a shaker at 30°C, 200rpm shaking, and obtain preliminary seed liquid after 16-18 hours. Acco...

Embodiment 2

[0050] Example 2 Cell scratch repair of Pichia pastoris ginseng fermentation product

[0051] To study the effect of 1% ginseng whole plant extract fermentation product of aerospace yeast SP02-DZ-08 on scratch injury of human keratinocytes.

[0052] Table 2 Samples and concentrations to be tested

[0053] Sample name full ingredients Final concentration 1% Ginseng Extract 1% Ginseng Extract 1％，0.05％ Fermentation product of 1% ginseng whole plant extract of SP02-DZ-08 1% Ginseng Extract Fermentation Broth 1％，0.05％

[0054] 1) Cell culture: Keratinocytes (AL17003FK P2), seeded in 6-well plates at 200,000 / well, 5% CO at 37°C 2 Cultured in a cell culture incubator for 3 days.

[0055] 2) Sample treatment: After the solution was filtered and sterilized before and after fermentation, it was diluted 100 and 200 times with medium, and the final test concentration was 1%. Cells were incubated at 37°C, 5% CO in medium containing the sample to be test...

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Abstract

The invention discloses a preparation method of a ginsenoside composition. The preparation method comprises the step of fermenting a whole ginseng plant extract by using pichia pastoris of CCTCC NO. M2016763 to obtain the ginsenoside composition. After the pichia pastoris is subjected to a specific biological fermentation conversion process, compared with a whole ginseng plant extract substrate,the ginsenoside composition has the characteristics that the lifting amplitudes of Rd, CK and R-Rg3 in a fermentation filtrate can respectively reach 9%, 37% and 2400%, so that a new direction and idea are provided for preparation of rare saponins in the field; and in addition, the obtained ginsenoside composition has stronger repair ability to keratinocytes.

Description

technical field [0001] The invention belongs to the field of bioengineering, and relates to a preparation method and application of a ginsenoside composition. Background technique [0002] Ginseng (Panax ginseng C.A.Mey.), a perennial herb of the Araliaceae genus Ginseng, was first recorded in "Shen Nong's Materia Medica". Tonic and strong medicine, the main active ingredient is ginsenoside. Ginsenoside is a kind of sterol compound. There are many monomer types in ginseng. It is a triterpenoid compound formed by linking sugar chains and aglycones. Ginsenosides can affect multiple metabolic pathways. Some rare human saponins can significantly prevent cancer, inhibit tumors and cancer cells, and are used in the field of anti-cancer clinical treatment and cancer drug development. saponin Rb in ginseng 1 , Rd, Re and other high content, while Rg 1 , Rg 3 、Rh 1 、Rh 2 etc. are rare ginsenosides with higher activity and unique effects in improving memory and preventing and t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P33/20C12N1/16A61K8/9789A61Q19/00A61Q19/08C12R1/84
CPCC12P33/20C12N1/16A61K8/9789A61Q19/00A61Q19/08A61K2800/85
Inventor 李俊章漳蒋耀权
Owner JALA GROUP CORPORATION
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