A kind of microbial inoculum suitable for industrialized large-scale composting and its application

A microbial inoculum and composting technology, which is applied in the directions of microorganism-based methods, microorganisms, and microbial treatment, can solve the problems of long composting cycle, insufficient turning depth and frequency of raking machines, and slow composting temperature, so as to improve the composting rate. efficiency, shorten the composting cycle, and promote the effect of heating

Active Publication Date: 2022-04-08
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the production of organic fertilizers, due to the improper ratio of compost raw materials and auxiliary materials, high moisture content, insufficient rake turning depth and times, or the influence of external low temperature, there are often problems such as slow compost temperature rise and excessive compost cycle. Long growth and low efficiency of organic fertilizer

Method used

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  • A kind of microbial inoculum suitable for industrialized large-scale composting and its application
  • A kind of microbial inoculum suitable for industrialized large-scale composting and its application
  • A kind of microbial inoculum suitable for industrialized large-scale composting and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Screening of functional strain Bacillus amyloliquefaciens Ba:

[0033] Existing strains isolated by the applicant from different habitats such as feces, compost, soil, etc., are screened for functional strains through analysis of strain enzyme production activity, antibiotic susceptibility test, in vitro antibacterial test, and analysis of the utilization ability of different nitrogen sources.

[0034] (1) Analysis of strain enzyme production activity

[0035] Inoculate the strains to be tested on casein medium, solid starch medium, and Congo red cellulose medium plates, and make a preliminary evaluation by comparing the ratio of the transparent circle to the colony diameter on the medium plate. The larger the ratio, the starch The higher the enzyme activity, the enzyme activity of each bacterial strain was combined for comparison. The final measured results of the strain Ba are shown in Table 1, Table 2 and Table 3, and the deposit number of the BS1 is CCTCC NO: M2019...

Embodiment 2

[0052] Preparation of microbial compound bacterial agent suitable for industrialized large-scale composting:

[0053] With Bacillus subtilis BS1 (CCTCC NO: M2019185), Bacillus amyloliquefaciens Ba, Bacillus licheniformis L 3 (Zhao Guowei, 2009) and Saccharomyces cerevisiae Y2 ( Jia Liyan , 2019) at a volume ratio of 3:1:3:4 and then obtained.

[0054] Before mixing, the effective bacterial concentration of Bacillus subtilis is (6.1~8.2)x10 8 CFU / mL, effective bacterial concentration of Bacillus amyloliquefaciens (2.6~3.07)x10 8 CFU / mL, the effective bacterial concentration of Bacillus licheniformis is (1.17~1.7)x10 8 CFU / mL, effective bacterial concentration of Saccharomyces cerevisiae (4.8~6.2)x10 7 CFU / mL.

[0055] After mixing, the number of viable bacteria in the composite bacterial agent was 7.60×10 8 CFU / mL, used in the following examples.

Embodiment 3

[0057] The application process of compound bacterial agent in small-scale composting of livestock and poultry manure in farms is as follows:

[0058] The composting method that the embodiment of the present invention uses is:

[0059] In order to maintain the temperature of the pile, the composting test device is an EPS foam box with a height of 280mm, a length of 540mm, a width of 400mm, a volume of 60L, and a thickness of 3mm. The bottom of the foam box is covered with about 3cm of sawdust as a buffer layer, and a small hole is opened on the side for temperature measurement. The ventilation device is regularly ventilated to ensure uniform ventilation.

[0060] The total volume of the pile is 50L. Samples are collected according to the temperature of the pile, and the temperature, C / N content, nitrate nitrogen, ammonium nitrogen, and nitrogenase gene changes of the pile are detected at the same time.

[0061]

[0062] The test groups are as follows:

[0063] Chicken manu...

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Abstract

The invention belongs to the field of microorganisms and their applications, and specifically discloses a microbial agent suitable for industrial large-scale composting and its application. The composite agent can be used for composting and fermenting livestock and poultry manure, and includes the following components: Bacillus subtilis Bacillus (BS1), Bacillus amyloliquefaciens (Ba), Bacillus licheniformis (L3), yeast (Y2). Among them, the Ba strain has been preserved in the China Center for Type Culture Collection, and the preservation number is: CCTCC M 2020098. The composite bacterial agent of the invention can increase the composting temperature during composting fermentation, accelerate the degradation of organic matter, promote the process of nitrogen conversion, shorten the composting cycle, fix nitrogen and improve the fertilizer efficiency of organic fertilizers.

Description

technical field [0001] The invention belongs to the field of microorganisms and applications thereof, and in particular relates to a composite bacterial agent and its application; the composite bacterial agent can be used for composting and fermenting livestock and poultry manure. Background technique [0002] With the continuous expansion of the total amount of livestock and poultry breeding in my country, the treatment of livestock and poultry manure has become a major problem. According to industry statistics, the country produces about 3.8 billion tons of livestock and poultry manure every year, of which about 1.8 billion tons are directly excreted by livestock and poultry. The large amount of livestock and poultry manure discharged and the difficulty of treatment have become one of the sources of agricultural non-point source pollution. It not only seriously affects the health and production performance of livestock and poultry, but also puts huge pressure on the surrou...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/18C05F3/00C05F11/08C05F17/20C12R1/125C12R1/07C12R1/10C12R1/865
CPCC12N1/20C12N1/18C05F3/00C05F11/08C05F17/20Y02W30/40Y02A40/20
Inventor 吴健曹蕊莫尚昆彭彦张雨薇黄钦林伏超兰任竹青
Owner HUAZHONG AGRI UNIV
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